Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106234
Name   oriT_pCY814036-KPC2 in_silico
Organism   Klebsiella pneumoniae strain CY814036
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP093153 (43076..43199 [+], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      101..106, 119..124  (TTTAAT..ATTAAA)
 91..99, 113..121  (AATAATGTA..TACATTATT)
 90..95, 107..112  (AAATAA..TTATTT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
 3..10, 15..22  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_pCY814036-KPC2
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTTTTTGAATCATTAGCTTATGTTTTAAATAATGTATTTTAATTTATTTTACATTATTAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4177 GenBank   WP_071177725
Name   traI_MMZ10_RS28290_pCY814036-KPC2 insolico UniProt ID   A0A7G3L5Q3
Length   1711 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1711 a.a.        Molecular weight: 186716.81 Da        Isoelectric Point: 6.1728

>WP_071177725.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAENRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLPEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNIYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDVQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDVMTVVVPGRAEPASLPVSDSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDKATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISHQKSALNTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFADLGREIDA
QIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETPDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISRDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPAQEQAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTSDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQVRERAAITERETALLQS

  Protein domains


Predicted by InterproScan.

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

TrwC relaxase (TrwC)

(10-283)

AAA domain (AAA_30)

(969-1156)

DNA helicase TraI (TraI_C)

(1434-1557)


  Protein structure



No available structure.




Auxiliary protein

ID   1863 GenBank   WP_000124981
Name   WP_000124981_pCY814036-KPC2 insolico UniProt ID   A0A630FTW9
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14542.59 Da        Isoelectric Point: 5.0278

>WP_000124981.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Gammaproteobacteria]
MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLELGLRVYDAQMERKESAFNQTEFNKLL
LECVVKTQSTVAKILGIESLSPHVSGNPKFEYASMVDDIREKVSVEMDRFFPKNDDE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A630FTW9

ID   1864 GenBank   WP_001254386
Name   WP_001254386_pCY814036-KPC2 insolico UniProt ID   A0A3Z6KJB5
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 9005.11 Da        Isoelectric Point: 10.1422

>WP_001254386.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Gammaproteobacteria]
MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVTEESES
TFKEL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(14-61)


  Protein structure


Source ID Structure
AlphaFold DB A0A3Z6KJB5


T4CP

ID   4194 GenBank   WP_015059012
Name   traC_MMZ10_RS28170_pCY814036-KPC2 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99393.22 Da        Isoelectric Point: 6.3465

>WP_015059012.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGVPFCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAA
TQFPLPEGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKKKQARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(290-446)

TraG P-loop domain (P-loop_TraG)

(467-771)

TraC protein (TraC_F_IV)

(38-276)

  Protein structure



No available structure.



ID   4195 GenBank   WP_000009363
Name   traD_MMZ10_RS28285_pCY814036-KPC2 insolico UniProt ID   _
Length   741 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 741 a.a.        Molecular weight: 84259.95 Da        Isoelectric Point: 5.0504

>WP_000009363.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMA
AYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 42504..71758

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMZ10_RS28065 (MMZ10_28065) 38476..38910 + 435 WP_000845953 conjugation system SOS inhibitor PsiB -
MMZ10_RS28070 (MMZ10_28070) 38907..39626 + 720 WP_001276217 plasmid SOS inhibition protein A -
MMZ10_RS28075 (MMZ10_28075) 39848..39997 + 150 Protein_51 plasmid maintenance protein Mok -
MMZ10_RS28080 (MMZ10_28080) 39939..40064 + 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
MMZ10_RS28085 (MMZ10_28085) 40383..40679 - 297 Protein_53 hypothetical protein -
MMZ10_RS28090 (MMZ10_28090) 40979..41275 + 297 WP_001272251 hypothetical protein -
MMZ10_RS28095 (MMZ10_28095) 41386..42207 + 822 WP_001234445 DUF932 domain-containing protein -
MMZ10_RS28100 (MMZ10_28100) 42504..43151 - 648 WP_015059008 transglycosylase SLT domain-containing protein virB1
MMZ10_RS28105 (MMZ10_28105) 43428..43811 + 384 WP_000124981 conjugal transfer relaxosome DNA-binding protein TraM -
MMZ10_RS28110 (MMZ10_28110) 44002..44688 + 687 WP_015059009 PAS domain-containing protein -
MMZ10_RS28115 (MMZ10_28115) 44782..45009 + 228 WP_001254386 conjugal transfer relaxosome protein TraY -
MMZ10_RS28120 (MMZ10_28120) 45043..45408 + 366 WP_021519752 type IV conjugative transfer system pilin TraA -
MMZ10_RS28125 (MMZ10_28125) 45423..45734 + 312 WP_000012106 type IV conjugative transfer system protein TraL traL
MMZ10_RS28130 (MMZ10_28130) 45756..46322 + 567 WP_000399794 type IV conjugative transfer system protein TraE traE
MMZ10_RS28135 (MMZ10_28135) 46309..47037 + 729 WP_001230787 type-F conjugative transfer system secretin TraK traK
MMZ10_RS28140 (MMZ10_28140) 47037..48464 + 1428 WP_032297072 F-type conjugal transfer pilus assembly protein TraB traB
MMZ10_RS28145 (MMZ10_28145) 48454..49044 + 591 WP_000002778 conjugal transfer pilus-stabilizing protein TraP -
MMZ10_RS28150 (MMZ10_28150) 49031..49228 + 198 WP_001324648 conjugal transfer protein TrbD -
MMZ10_RS28155 (MMZ10_28155) 49240..49491 + 252 WP_001038341 conjugal transfer protein TrbG -
MMZ10_RS28160 (MMZ10_28160) 49488..50003 + 516 WP_062955122 type IV conjugative transfer system lipoprotein TraV traV
MMZ10_RS28165 (MMZ10_28165) 50138..50359 + 222 WP_001278692 conjugal transfer protein TraR -
MMZ10_RS28170 (MMZ10_28170) 50519..53146 + 2628 WP_015059012 type IV secretion system protein TraC virb4
MMZ10_RS28175 (MMZ10_28175) 53143..53529 + 387 WP_015059013 type-F conjugative transfer system protein TrbI -
MMZ10_RS28180 (MMZ10_28180) 53526..54158 + 633 WP_001203735 type-F conjugative transfer system protein TraW traW
MMZ10_RS28185 (MMZ10_28185) 54155..55147 + 993 WP_000830838 conjugal transfer pilus assembly protein TraU traU
MMZ10_RS28190 (MMZ10_28190) 55174..55482 + 309 WP_000412447 hypothetical protein -
MMZ10_RS28195 (MMZ10_28195) 55545..55805 + 261 Protein_75 hypothetical protein -
MMZ10_RS28200 (MMZ10_28200) 55839..56807 + 969 WP_013362812 IS5-like element IS903B family transposase -
MMZ10_RS28205 (MMZ10_28205) 56866..57132 + 267 Protein_77 hypothetical protein -
MMZ10_RS28210 (MMZ10_28210) 57159..57797 + 639 WP_000087532 type-F conjugative transfer system pilin assembly protein TrbC trbC
MMZ10_RS28215 (MMZ10_28215) 57794..58165 + 372 WP_000056336 hypothetical protein -
MMZ10_RS28220 (MMZ10_28220) 58191..58613 + 423 WP_001229309 HNH endonuclease signature motif containing protein -
MMZ10_RS28225 (MMZ10_28225) 58610..60460 + 1851 WP_015059015 type-F conjugative transfer system mating-pair stabilization protein TraN traN
MMZ10_RS28230 (MMZ10_28230) 60487..60744 + 258 WP_053913405 conjugal transfer protein TrbE -
MMZ10_RS28235 (MMZ10_28235) 60731..61480 + 750 WP_072354014 type-F conjugative transfer system pilin assembly protein TraF traF
MMZ10_RS28240 (MMZ10_28240) 61641..61877 + 237 WP_000270010 type-F conjugative transfer system pilin chaperone TraQ -
MMZ10_RS28245 (MMZ10_28245) 61864..62409 + 546 WP_000059817 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
MMZ10_RS28250 (MMZ10_28250) 62399..62713 + 315 WP_001384255 P-type conjugative transfer protein TrbJ -
MMZ10_RS28255 (MMZ10_28255) 62667..63059 + 393 WP_000126338 F-type conjugal transfer protein TrbF -
MMZ10_RS28260 (MMZ10_28260) 63046..64419 + 1374 WP_000944325 conjugal transfer pilus assembly protein TraH traH
MMZ10_RS28265 (MMZ10_28265) 64416..67232 + 2817 WP_015059018 conjugal transfer mating-pair stabilization protein TraG traG
MMZ10_RS28270 (MMZ10_28270) 67265..67786 + 522 WP_000632668 conjugal transfer entry exclusion protein TraS -
MMZ10_RS28275 (MMZ10_28275) 67811..68542 + 732 WP_021559024 conjugal transfer complement resistance protein TraT -
MMZ10_RS28280 (MMZ10_28280) 68745..69482 + 738 WP_015059020 hypothetical protein -
MMZ10_RS28285 (MMZ10_28285) 69533..71758 + 2226 WP_000009363 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   6671 GenBank   NZ_CP093153
Plasmid name   pCY814036-KPC2 Incompatibility group   IncR
Plasmid size   140105 bp Coordinate of oriT [Strand]   43076..43199 [+]
Host baterium   Klebsiella pneumoniae strain CY814036

Cargo genes

Drug resistance gene   blaKPC-2, blaCTX-M-65, blaTEM-1B, rmtB
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9