Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106212
Name   oriT_pSW25H01 in_silico
Organism   Klebsiella pneumoniae strain AHSWKP25
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP091053 (32948..33047 [-], 100 nt)
oriT length   100 nt
IRs (inverted repeats)      34..41, 44..51  (AGTGCGAT..ATCGCACT)
 20..25, 39..44  (TAAATC..GATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 100 nt

>oriT_pSW25H01
TATTGAATGTTTTATTCTTTAAATCAGTTAGTTAGTGCGATTTATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATCAGTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4163 GenBank   WP_020323154
Name   mobF_L1P15_RS28825_pSW25H01 insolico UniProt ID   A0A0R8CGS3
Length   1072 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1072 a.a.        Molecular weight: 118689.40 Da        Isoelectric Point: 6.4933

>WP_020323154.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNGQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFHVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGATEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKGRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHDDGQQAKSYGHEAGQQTGEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDYDKEGYGL

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(16-288)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(855-899)

AAA domain (AAA_30)

(480-656)


  Protein structure


Source ID Structure
AlphaFold DB A0A0R8CGS3


T4CP

ID   4182 GenBank   WP_020323171
Name   t4cp2_L1P15_RS28820_pSW25H01 insolico UniProt ID   _
Length   510 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 510 a.a.        Molecular weight: 58208.12 Da        Isoelectric Point: 9.7037

>WP_020323171.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVSIIAGSVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAEKTKERGHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGRDKRVILDPNGEYVETFYRENDIILNPYDKRSPGWSMFNEIRDEFDYDLYAVSIIQ
ESKERSAEEWYRYGRLLYREVSKKLLTHKRNPSMEEVLYWCNVAEYDKVKDFVKGTPAESLFIKGAERAT
GSARFVLGDKLASHLKMPEGNFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTFSQLVERYGINIAETLLGSMRSGIVMGSSRL
GKATLEHMSRALGEIEGEIERRQGSLLQSGPNNRPRIETRTVRAVTPTEISELRNLTGYIYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-492)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 33618..51114

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P15_RS28780 (L1P15_28780) 29405..30082 + 678 WP_020323167 DUF5417 domain-containing protein -
L1P15_RS28785 (L1P15_28785) 30264..30470 + 207 WP_020323152 DUF905 family protein -
L1P15_RS28790 (L1P15_28790) 30544..30726 + 183 WP_000170167 hypothetical protein -
L1P15_RS28795 (L1P15_28795) 30785..31135 - 351 WP_223226687 hypothetical protein -
L1P15_RS28800 (L1P15_28800) 31199..31567 - 369 WP_032416596 plasmid stabilization protein StbC -
L1P15_RS28805 (L1P15_28805) 31570..32286 - 717 WP_020323147 StbB family protein -
L1P15_RS28810 (L1P15_28810) 32295..32726 - 432 WP_020323173 plasmid stabilization protein StbA -
L1P15_RS28815 (L1P15_28815) 33142..33618 + 477 WP_223226686 traK protein -
L1P15_RS28820 (L1P15_28820) 33618..35150 + 1533 WP_020323171 type IV secretion system DNA-binding domain-containing protein virb4
L1P15_RS28825 (L1P15_28825) 35155..38373 + 3219 WP_020323154 MobF family relaxase -
L1P15_RS28830 (L1P15_28830) 38370..39035 + 666 WP_020323157 DUF6710 family protein -
L1P15_RS28835 (L1P15_28835) 39028..39390 + 363 WP_020323170 hypothetical protein -
L1P15_RS28840 (L1P15_28840) 40006..40317 - 312 WP_032416518 hypothetical protein -
L1P15_RS28845 (L1P15_28845) 40314..40781 - 468 WP_046092601 phospholipase D family protein -
L1P15_RS28850 (L1P15_28850) 40838..41839 - 1002 WP_020323165 ATPase, T2SS/T4P/T4SS family virB11
L1P15_RS28855 (L1P15_28855) 41890..43023 - 1134 WP_020323153 type IV secretion system protein VirB10 virB10
L1P15_RS28860 (L1P15_28860) 43023..43910 - 888 WP_020323166 TrbG/VirB9 family P-type conjugative transfer protein virB9
L1P15_RS28865 (L1P15_28865) 43921..44616 - 696 WP_001208352 type IV secretion system protein virB8
L1P15_RS29120 44627..44758 - 132 WP_032493177 hypothetical protein -
L1P15_RS28870 (L1P15_28870) 44835..45872 - 1038 WP_020323169 type IV secretion system protein virB6
L1P15_RS28875 (L1P15_28875) 45889..46131 - 243 WP_000858958 EexN family lipoprotein -
L1P15_RS28880 (L1P15_28880) 46141..46842 - 702 WP_020323143 type IV secretion system protein virB5
L1P15_RS28885 (L1P15_28885) 46858..49431 - 2574 WP_020323176 VirB4 family type IV secretion/conjugal transfer ATPase virb4
L1P15_RS28890 (L1P15_28890) 49432..49749 - 318 WP_000462629 VirB3 family type IV secretion system protein virB3
L1P15_RS28895 (L1P15_28895) 49789..50130 - 342 WP_001121032 TrbC/VirB2 family protein virB2
L1P15_RS28900 (L1P15_28900) 50332..51114 - 783 WP_032416587 lytic transglycosylase domain-containing protein virB1
L1P15_RS28905 (L1P15_28905) 51214..51531 + 318 WP_032449361 H-NS histone family protein -
L1P15_RS28910 (L1P15_28910) 51535..51885 + 351 WP_032416642 hypothetical protein -
L1P15_RS28915 (L1P15_28915) 51882..52220 + 339 WP_223226685 hypothetical protein -
L1P15_RS28920 (L1P15_28920) 52224..52532 + 309 WP_032416643 hypothetical protein -
L1P15_RS28925 (L1P15_28925) 52817..53002 + 186 WP_006118530 ribbon-helix-helix domain-containing protein -
L1P15_RS28930 (L1P15_28930) 52999..53607 - 609 WP_032449362 recombinase family protein -
L1P15_RS28935 (L1P15_28935) 53773..54105 - 333 WP_020323168 hypothetical protein -

Host bacterium

ID   6649 GenBank   NZ_CP091053
Plasmid name   pSW25H01 Incompatibility group   IncN3
Plasmid size   54244 bp Coordinate of oriT [Strand]   32948..33047 [-]
Host baterium   Klebsiella pneumoniae strain AHSWKP25

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -