Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106206
Name   oriT_pW49-2-a in_silico
Organism   Escherichia marmotae strain W49-2
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP093240 (49231..49314 [+], 84 nt)
oriT length   84 nt
IRs (inverted repeats)      28..33, 37..42  (GCCATC..GATGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 84 nt

>oriT_pW49-2-a
ACGGGACAGGATATGTTTTGTTGAACCGCCATCTTCGATGGCCGCTCAACAAAACCCCTGGTCAGGGCGCAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4157 GenBank   WP_241556898
Name   Relaxase_MNY74_RS24790_pW49-2-a insolico UniProt ID   _
Length   644 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 644 a.a.        Molecular weight: 74968.88 Da        Isoelectric Point: 6.4714

>WP_241556898.1 TraI/MobA(P) family conjugative relaxase [Escherichia marmotae]
MDKKPGELLSPDNPYVRPVRSREAIVEHLGQFIEEGMDLYRNHVLSRSDDGRQQVLCGEVLCETNCFGIE
TAAAEMDAVASQNRRCQDPVYHAILSWREEDNPTNEQIFECARYALKALNMSDHQYVFAIHRDTEHVHCH
ITVNRINPKTYRAASLYDDYFRLHKACRELEQKYGFTPDNGCCQKDENGNWIRKQEEFKSIPRKARQMEY
HADKESLYSYAVDEARHTIGKILHSGEATWEALHTELIRLGLELKEKGEGLAVYSRHDDTVTPIKASTLH
PDLTKRVLEPYLGKFTPSPVVENYFDENDNYLGSNYPQEYVYDVRAHKRDIGASSARREERADAREALKA
RYNAYRAGWTRPEMDRDEIRARFYHVSGQFAWRRAQVRAEIRDPLLRKLAYNVLALERRQAMIALKETLR
EERKAFYARPENRRMGYWQWVEQQALNNDAAAISCLRGRFYMQKKMQQENRLSENAIVCAVADDIRPYEI
EGYQTQITRDGRIQYCQDGEVKLQDTGDRIEIADPYSQDGQHIAGAMVLAEVKSGERMYFAGEPQFVNKA
CSIVPWFNEGSEKPLPLTDRAQRQMAGYDTAPASPQKDEPEIAWESLRWLMEGAPKNPVQEDVRQQQKHD
YVHDDNYSSRYRRH

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(63-289)

Large polyvalent protein-associated domain 7 (LPD7)

(493-563)


  Protein structure



No available structure.




T4CP

ID   4177 GenBank   WP_193803150
Name   trbC_MNY74_RS24985_pW49-2-a insolico UniProt ID   _
Length   738 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 738 a.a.        Molecular weight: 83684.13 Da        Isoelectric Point: 7.2887

>WP_193803150.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Escherichia]
MAIDSRPVDKQRVQHVIKSTWLMDILQSPFSLQAGMLGGLLVCLIAPWLLLPCLICAVIWLLVFTDQRFR
LPLRMPTDIGGFDYSTEREEANEFTLPGGFFRFIRRKLTVEKAGGIMCLGYARKRFLGRELWLTRDDCLR
HMQLLATTGSGKTEALLSLQLNAICLGRGYCYSDGKAETALAFSSWSLLRRFGLEDNFYVLNFLNGGRDR
FDELLSNDRTRPQSNTINLSNNATASYIIQLMDSMLPKVDGSSADWQDKARPMLYGLVYSLHYKCRRENL
RFSLAMLHKHLPLKEMAALYIEGKQNGWHQEGINALENYLSTLAGFQMDLVSRPAEWDQGVFDQHGFLIQ
QFSKMLAMFNDVYGHIFANDAGDIDLRDVLHNDRALVVLIPALELSKSESASLGKLYISAKRMVIARDLG
YHLEGKREEVLTAKKYRNRFPFPDIHDELGSYFAPGMDDLAAQMRSLGYMLIISAQDIQRFIAQFRGEYQ
TVNANLLVKWFMALQDEKDTYELARSTAGKDYFAELGEVKRSPGLFGNQFEDATTTHIREKDRISLDELK
NLNPGEGFISFKSALVPCNAVYIPDDEKLSSKLQMRINRFIDIGRPSESELVKKNPSLARLLPPDSQEVS
LVLQRTDEIMAGMAPRNPPPVVDPILARLAEITLNLDNRCDISYTPEQRGILLFEAAREAMKRSRGRWFL
AQTRHRPISVSDQVAHKLATDSTAFPSPVNTDKGTDPQ

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 86035..115544

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY74_RS24980 (MNY74_24980) 83299..83799 - 501 WP_241556901 phospholipase D family protein -
MNY74_RS24985 (MNY74_24985) 83829..86045 - 2217 WP_193803150 F-type conjugative transfer protein TrbC -
MNY74_RS24990 (MNY74_24990) 86035..86955 - 921 WP_193803149 conjugal transfer protein TraF trbB
MNY74_RS24995 (MNY74_24995) 86965..88098 - 1134 WP_241556879 conjugal transfer protein TrbA trbA
MNY74_RS25000 (MNY74_25000) 88114..88896 - 783 WP_001317493 IS21-like element IS100kyp family helper ATPase IstB -
MNY74_RS25005 (MNY74_25005) 88893..89915 - 1023 WP_000255956 IS21-like element IS100 family transposase -
MNY74_RS25010 (MNY74_25010) 89929..90297 - 369 WP_241556880 hypothetical protein trbA
MNY74_RS25015 (MNY74_25015) 90584..90721 - 138 WP_107135979 Hok/Gef family protein -
MNY74_RS25020 (MNY74_25020) 91081..91734 - 654 WP_105289555 DUF5710 domain-containing protein -
MNY74_RS25025 (MNY74_25025) 91774..92130 - 357 WP_107135981 hypothetical protein -
MNY74_RS25030 (MNY74_25030) 92384..92584 - 201 WP_097468676 hemolysin expression modulator Hha -
MNY74_RS25035 (MNY74_25035) 92647..93183 - 537 WP_170898668 hypothetical protein -
MNY74_RS25040 (MNY74_25040) 93323..93871 - 549 WP_241556881 hypothetical protein -
MNY74_RS25045 (MNY74_25045) 93886..96021 - 2136 WP_193803147 DotA/TraY family protein traY
MNY74_RS25050 (MNY74_25050) 96105..96449 - 345 WP_215251741 hypothetical protein -
MNY74_RS25055 (MNY74_25055) 96451..96951 - 501 WP_193803146 hypothetical protein -
MNY74_RS25060 (MNY74_25060) 97118..97411 - 294 WP_063848353 type II toxin-antitoxin system RelE/ParE family toxin -
MNY74_RS25065 (MNY74_25065) 97411..97683 - 273 WP_063848352 type II toxin-antitoxin system Phd/YefM family antitoxin -
MNY74_RS25070 (MNY74_25070) 97820..97966 - 147 WP_181475174 hypothetical protein -
MNY74_RS25075 (MNY74_25075) 98111..98641 - 531 WP_170898664 conjugal transfer protein TraX -
MNY74_RS25080 (MNY74_25080) 98638..99858 - 1221 WP_001039317 conjugal transfer protein TraW traW
MNY74_RS25085 (MNY74_25085) 99882..102938 - 3057 WP_241556882 ATP-binding protein traU
MNY74_RS25090 (MNY74_25090) 102942..103553 - 612 WP_241556883 hypothetical protein traT
MNY74_RS25095 (MNY74_25095) 103547..103816 - 270 WP_241556884 hypothetical protein -
MNY74_RS25100 (MNY74_25100) 103872..104279 - 408 WP_001059622 DUF6750 family protein traR
MNY74_RS25105 (MNY74_25105) 104311..104835 - 525 WP_241556902 conjugal transfer protein TraQ traQ
MNY74_RS25110 (MNY74_25110) 104846..105547 - 702 WP_241556885 conjugal transfer protein TraP traP
MNY74_RS25115 (MNY74_25115) 105548..106741 - 1194 WP_241556886 conjugal transfer protein TraO traO
MNY74_RS25120 (MNY74_25120) 106744..107769 - 1026 WP_193803144 DotH/IcmK family type IV secretion protein traN
MNY74_RS25125 (MNY74_25125) 107766..108437 - 672 WP_050939885 DotI/IcmL/TraM family protein traM
MNY74_RS25130 (MNY74_25130) 108457..108936 - 480 WP_170898655 hypothetical protein traL
MNY74_RS25135 (MNY74_25135) 108951..112394 - 3444 WP_241556887 DUF5710 domain-containing protein -
MNY74_RS25140 (MNY74_25140) 112412..112672 - 261 WP_050939877 IcmT/TraK family protein traK
MNY74_RS25145 (MNY74_25145) 112665..113837 - 1173 WP_241556888 plasmid transfer ATPase TraJ virB11
MNY74_RS25150 (MNY74_25150) 113843..114625 - 783 WP_105289544 type IV secretory system conjugative DNA transfer family protein traI
MNY74_RS25155 (MNY74_25155) 114622..115047 - 426 WP_157945705 DotD/TraH family lipoprotein -
MNY74_RS25160 (MNY74_25160) 115074..115544 - 471 WP_157917732 lytic transglycosylase domain-containing protein virB1
MNY74_RS25165 (MNY74_25165) 115544..116041 - 498 WP_105289542 hypothetical protein -
MNY74_RS25170 (MNY74_25170) 116051..116293 - 243 WP_105289541 hypothetical protein -
MNY74_RS25175 (MNY74_25175) 116432..116632 - 201 WP_105289540 hypothetical protein -
MNY74_RS25180 (MNY74_25180) 116622..117299 - 678 WP_241556889 hypothetical protein -
MNY74_RS25185 (MNY74_25185) 117397..117909 - 513 WP_105289538 transcription termination/antitermination NusG family protein -

Host bacterium

ID   6643 GenBank   NZ_CP093240
Plasmid name   pW49-2-a Incompatibility group   IncI1
Plasmid size   118621 bp Coordinate of oriT [Strand]   49231..49314 [+]
Host baterium   Escherichia marmotae strain W49-2

Cargo genes

Drug resistance gene   -
Virulence gene   faeC, faeD, faeE, faeF, faeH, faeI, faeJ, papD, papC, papH
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -