Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106124 |
| Name | oriT_pESI  |
| Organism | Salmonella enterica subsp. enterica serovar Muenchen strain 180135033 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP088902 (230929..231017 [+], 89 nt) |
| oriT length | 89 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 89 nt
>oriT_pESI
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4103 | GenBank | WP_001299212 |
| Name | nikB_LRP35_RS24805_pESI |
UniProt ID | A0A5T4AK12 |
| Length | 899 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 103956.40 Da Isoelectric Point: 7.2642
>WP_001299212.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWINFYGVTCFHNCTSLETAAADMEYIALQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVSDWLSLHRRLAEDGLYLSQMDGK
YLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWINFYGVTCFHNCTSLETAAADMEYIALQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVSDWLSLHRRLAEDGLYLSQMDGK
YLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5T4AK12 |
Auxiliary protein
| ID | 579 | GenBank | WP_001283947 |
| Name | WP_001283947_pESI |
UniProt ID | A0A142CMC2 |
| Length | 110 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 110 a.a. Molecular weight: 12613.57 Da Isoelectric Point: 10.7463
>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142CMC2 |
T4CP
| ID | 4117 | GenBank | WP_001289276 |
| Name | trbC_LRP35_RS24800_pESI |
UniProt ID | _ |
| Length | 763 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 763 a.a. Molecular weight: 86941.11 Da Isoelectric Point: 6.7713
>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 189409..225551
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LRP35_RS24595 (LRP35_24600) | 184471..185538 | + | 1068 | WP_001360287 | type IV pilus biogenesis lipoprotein PilL | - |
| LRP35_RS24600 (LRP35_24605) | 185538..185975 | + | 438 | WP_000539807 | type IV pilus biogenesis protein PilM | - |
| LRP35_RS24605 (LRP35_24610) | 185989..187671 | + | 1683 | WP_000748143 | PilN family type IVB pilus formation outer membrane protein | - |
| LRP35_RS24610 (LRP35_24615) | 187664..188959 | + | 1296 | WP_001545757 | type 4b pilus protein PilO2 | - |
| LRP35_RS24615 (LRP35_24620) | 188946..189398 | + | 453 | WP_001247331 | type IV pilus biogenesis protein PilP | - |
| LRP35_RS24620 (LRP35_24625) | 189409..190962 | + | 1554 | WP_000362206 | ATPase, T2SS/T4P/T4SS family | virB11 |
| LRP35_RS24625 (LRP35_24630) | 190975..192060 | + | 1086 | WP_001208802 | type II secretion system F family protein | - |
| LRP35_RS24630 (LRP35_24635) | 192077..192691 | + | 615 | WP_000908226 | type 4 pilus major pilin | - |
| LRP35_RS24635 (LRP35_24640) | 192701..193261 | + | 561 | WP_000014005 | lytic transglycosylase domain-containing protein | virB1 |
| LRP35_RS24640 (LRP35_24645) | 193246..193902 | + | 657 | WP_001193549 | A24 family peptidase | - |
| LRP35_RS24645 (LRP35_24650) | 193902..195194 | + | 1293 | WP_001350014 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| LRP35_RS24650 (LRP35_24655) | 195250..195375 | - | 126 | Protein_182 | RNA-guided endonuclease TnpB family protein | - |
| LRP35_RS24655 (LRP35_24660) | 195583..196407 | + | 825 | WP_001238942 | conjugal transfer protein TraE | traE |
| LRP35_RS24660 (LRP35_24665) | 196493..197695 | + | 1203 | WP_000976348 | conjugal transfer protein TraF | - |
| LRP35_RS24665 (LRP35_24670) | 197755..198339 | + | 585 | WP_000977522 | histidine phosphatase family protein | - |
| LRP35_RS24670 (LRP35_24675) | 198734..199192 | + | 459 | WP_001079808 | IncI1-type conjugal transfer lipoprotein TraH | - |
| LRP35_RS24675 (LRP35_24680) | 199189..200007 | + | 819 | WP_000646097 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| LRP35_RS24680 (LRP35_24685) | 200004..201152 | + | 1149 | WP_001024972 | plasmid transfer ATPase TraJ | virB11 |
| LRP35_RS24685 (LRP35_24690) | 201149..201439 | + | 291 | WP_001299214 | hypothetical protein | traK |
| LRP35_RS24690 (LRP35_24695) | 201454..202005 | + | 552 | WP_000014584 | phospholipase D family protein | - |
| LRP35_RS24695 (LRP35_24700) | 202095..205862 | + | 3768 | WP_001141542 | LPD7 domain-containing protein | - |
| LRP35_RS24700 (LRP35_24705) | 205880..206227 | + | 348 | WP_001055900 | conjugal transfer protein | traL |
| LRP35_RS24705 (LRP35_24710) | 206224..206916 | + | 693 | WP_000138552 | DotI/IcmL family type IV secretion protein | traM |
| LRP35_RS24710 (LRP35_24715) | 206927..207910 | + | 984 | WP_001191878 | IncI1-type conjugal transfer protein TraN | traN |
| LRP35_RS24715 (LRP35_24720) | 207913..209202 | + | 1290 | WP_001271997 | conjugal transfer protein TraO | traO |
| LRP35_RS24720 (LRP35_24725) | 209202..209906 | + | 705 | WP_000801920 | IncI1-type conjugal transfer protein TraP | traP |
| LRP35_RS24725 (LRP35_24730) | 209906..210433 | + | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| LRP35_RS24730 (LRP35_24735) | 210484..210888 | + | 405 | WP_000086961 | IncI1-type conjugal transfer protein TraR | traR |
| LRP35_RS24735 (LRP35_24740) | 210952..211140 | + | 189 | WP_001277255 | putative conjugal transfer protein TraS | - |
| LRP35_RS24740 (LRP35_24745) | 211124..211924 | + | 801 | WP_001164788 | IncI1-type conjugal transfer protein TraT | traT |
| LRP35_RS24745 (LRP35_24750) | 212014..215058 | + | 3045 | WP_001024780 | IncI1-type conjugal transfer protein TraU | traU |
| LRP35_RS24750 (LRP35_24755) | 215058..215672 | + | 615 | WP_000337400 | IncI1-type conjugal transfer protein TraV | traV |
| LRP35_RS24755 (LRP35_24760) | 215639..216841 | + | 1203 | WP_001189156 | IncI1-type conjugal transfer protein TraW | traW |
| LRP35_RS24760 (LRP35_24765) | 216870..217454 | + | 585 | WP_001037987 | IncI1-type conjugal transfer protein TraX | - |
| LRP35_RS24765 (LRP35_24770) | 217551..219719 | + | 2169 | WP_000698357 | DotA/TraY family protein | traY |
| LRP35_RS24770 (LRP35_24775) | 219793..220443 | + | 651 | WP_001178506 | plasmid IncI1-type surface exclusion protein ExcA | - |
| LRP35_RS24775 (LRP35_24780) | 220515..220724 | - | 210 | WP_000062603 | HEAT repeat domain-containing protein | - |
| LRP35_RS24780 (LRP35_24785) | 221116..221292 | + | 177 | WP_001054904 | hypothetical protein | - |
| LRP35_RS25200 | 221357..221452 | - | 96 | WP_001303310 | DinQ-like type I toxin DqlB | - |
| LRP35_RS24785 (LRP35_24790) | 222084..222992 | + | 909 | WP_000055084 | hypothetical protein | - |
| LRP35_RS24790 (LRP35_24795) | 223254..224462 | + | 1209 | WP_000121273 | IncI1-type conjugal transfer protein TrbA | trbA |
| LRP35_RS24795 (LRP35_24800) | 224481..225551 | + | 1071 | WP_000151577 | IncI1-type conjugal transfer protein TrbB | trbB |
| LRP35_RS24800 (LRP35_24805) | 225544..227835 | + | 2292 | WP_001289276 | F-type conjugative transfer protein TrbC | - |
Host bacterium
| ID | 6561 | GenBank | NZ_CP088902 |
| Plasmid name | pESI | Incompatibility group | IncFIB |
| Plasmid size | 285167 bp | Coordinate of oriT [Strand] | 230929..231017 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Muenchen strain 180135033 |
Cargo genes
| Drug resistance gene | ant(3'')-Ia, qacE, sul1, tet(A), dfrA14 |
| Virulence gene | faeC, faeD, faeE, faeF, faeH, faeI, fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | merE, merD, merA, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |