Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106109
Name   oriT_pZA25-tetX-168kb in_silico
Organism   Proteus mirabilis strain ZA25
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP047353 (86504..86654 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pZA25-tetX-168kb
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4094 GenBank   WP_031969001
Name   mobH_GTH26_RS18855_pZA25-tetX-168kb insolico UniProt ID   _
Length   978 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 978 a.a.        Molecular weight: 108140.11 Da        Isoelectric Point: 4.8256

>WP_031969001.1 MobH family relaxase [Proteus mirabilis]
MIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGFTVDDFNRLVLPVIQ
RYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNEPRWRLASCFSGLLH
DVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSLLAVDRIIPAETREF
LSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVPVERYVFDAIRRLVK
TGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQEKGER
AYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIEFVDPEEADDGDDQE
EGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSKPDAGKKGKPQSKPG
KAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDAFNASAETTSTDA
TNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLTDAPEQPTHTPEMPH
TDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPDGARSLGAPSE
VLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDAFELVSPPGLDAS
KNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQRKPVQEKQNVARLP
KREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSGVTPQKALQMLKDMI
QKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(37-343)


  Protein structure



No available structure.




T4CP

ID   4107 GenBank   WP_000178856
Name   traD_GTH26_RS18860_pZA25-tetX-168kb insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   4108 GenBank   WP_000637386
Name   traC_GTH26_RS18940_pZA25-tetX-168kb insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 84588..109779

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH26_RS18845 (GTH26_18750) 80613..80846 - 234 WP_001191892 hypothetical protein -
GTH26_RS18850 (GTH26_18755) 80828..81445 - 618 WP_001249396 hypothetical protein -
GTH26_RS18855 (GTH26_18760) 81613..84591 + 2979 WP_001337757 MobH family relaxase -
GTH26_RS18860 (GTH26_18765) 84588..86453 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
GTH26_RS18865 (GTH26_18770) 86503..87048 + 546 WP_000228720 hypothetical protein -
GTH26_RS18870 (GTH26_18775) 87005..87634 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
GTH26_RS19400 87682..87858 + 177 WP_169342285 hypothetical protein -
GTH26_RS18880 (GTH26_18785) 88100..88477 + 378 WP_000869261 hypothetical protein -
GTH26_RS18885 (GTH26_18790) 88477..89139 + 663 WP_001231463 hypothetical protein -
GTH26_RS18890 89320..89463 + 144 WP_001275801 hypothetical protein -
GTH26_RS18895 (GTH26_18795) 89475..89840 + 366 WP_001052531 hypothetical protein -
GTH26_RS18900 (GTH26_18800) 89985..90266 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
GTH26_RS18905 (GTH26_18805) 90263..90889 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
GTH26_RS18910 (GTH26_18810) 90873..91790 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
GTH26_RS18915 (GTH26_18815) 91790..93103 + 1314 WP_024131605 TraB/VirB10 family protein traB
GTH26_RS18920 (GTH26_18820) 93100..93678 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
GTH26_RS18925 (GTH26_18825) 93682..94074 + 393 WP_000479535 TraA family conjugative transfer protein -
GTH26_RS18930 (GTH26_18830) 94275..99806 + 5532 WP_000606833 Ig-like domain-containing protein -
GTH26_RS18935 (GTH26_18835) 99955..100662 + 708 WP_001259347 DsbC family protein -
GTH26_RS18940 (GTH26_18840) 100659..103106 + 2448 WP_000637386 type IV secretion system protein TraC virb4
GTH26_RS18945 (GTH26_18845) 103121..103438 + 318 WP_000351984 hypothetical protein -
GTH26_RS18950 (GTH26_18850) 103435..103965 + 531 WP_001010738 S26 family signal peptidase -
GTH26_RS18955 (GTH26_18855) 103928..105193 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
GTH26_RS18960 (GTH26_18860) 105190..105861 + 672 WP_001337754 EAL domain-containing protein -
GTH26_RS18965 (GTH26_18865) 105861..106865 + 1005 WP_043940352 TraU family protein traU
GTH26_RS18970 (GTH26_18870) 106981..109779 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
GTH26_RS18975 (GTH26_18875) 109818..110678 - 861 WP_000709517 hypothetical protein -
GTH26_RS18980 (GTH26_18880) 110801..111442 - 642 WP_000796664 hypothetical protein -
GTH26_RS18985 (GTH26_18885) 111736..112056 + 321 WP_000547566 hypothetical protein -
GTH26_RS18990 (GTH26_18890) 112360..112545 + 186 WP_001186917 hypothetical protein -
GTH26_RS18995 (GTH26_18895) 112765..113733 + 969 WP_000085162 AAA family ATPase -
GTH26_RS19000 (GTH26_18900) 113744..114652 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   6546 GenBank   NZ_CP047353
Plasmid name   pZA25-tetX-168kb Incompatibility group   IncA/C2
Plasmid size   168512 bp Coordinate of oriT [Strand]   86504..86654 [+]
Host baterium   Proteus mirabilis strain ZA25

Cargo genes

Drug resistance gene   aph(3')-Ia, aac(3)-IId, blaTEM-1B, aph(6)-Id, aph(3'')-Ib, tet(X6), floR, sul2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -