Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   106079
Name   oriT_GY8|p1 in_silico
Organism   Staphylococcus aureus strain GY8
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP092826 (10962..11201 [-], 240 nt)
oriT length   240 nt
IRs (inverted repeats)      217..222, 232..237  (ATTTTA..TAAAAT)
 171..178, 183..190  (CTATCATT..AATGATAG)
 154..160, 164..170  (GTCTGGC..GCCAGAC)
 26..32, 44..50  (TTTTTTA..TAAAAAA)
 37..42, 45..50  (TTTTTT..AAAAAA)
 1..7, 20..26  (AAGACAT..ATGTCTT)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 240 nt

>oriT_GY8|p1
AAGACATTAGTGATAACTGATGTCTTTTTTTATTGATTTTTTGTAAAAAAGTACTGTCTTAGTTTTGTGACAAATACTGTATATAGTGTCACAAAAGTGTGACACTACAGCTTTTTATGATATCACTTTAAAATTACTTAAAACCCTTGGAATGTCTGGCTTTGCCAGACCTATCATTTTTGAATGATAGCAAATTCCCCTTATGCTCTTACGGAGATTTTAGAGTTAAATTAAAATTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   4080 GenBank   WP_000122374
Name   mobV_MK796_RS13855_GY8|p1 insolico UniProt ID   A0A2R4A9A7
Length   413 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 413 a.a.        Molecular weight: 48526.00 Da        Isoelectric Point: 6.1679

>WP_000122374.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNYDLVNANKQNFNNLIDEKIEQNYTGK
RKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVP
ITDDGRLSAKEVVGNKKALTAFQDRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERE
SQKTDHIKQKNDKLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKA
AQDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIEIALKLLKILL
KELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQEQQKNQKRDRGMHL

  Protein domains


Predicted by InterproScan.

(1-198)


  Protein structure


Source ID Structure
AlphaFold DB A0A2R4A9A7


Host bacterium


ID   6516 GenBank   NZ_CP092826
Plasmid name   GY8|p1 Incompatibility group   -
Plasmid size   25884 bp Coordinate of oriT [Strand]   10962..11201 [-]
Host baterium   Staphylococcus aureus strain GY8

Cargo genes


Drug resistance gene   blaZ, tet(K)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21