Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106052 |
| Name | oriT_pMUP32b  |
| Organism | Pseudomonas syringae strain MUP32 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP110811 (47783..48003 [+], 221 nt) |
| oriT length | 221 nt |
| IRs (inverted repeats) | 124..131, 135..142 (CAAAGGGG..CCCCTTTG) 86..92, 102..108 (CGGGGGG..CCCCCCG) 87..92, 101..106 (GGGGGG..CCCCCC) 32..38, 40..46 (TTGGGTC..GACCCAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 221 nt
>oriT_pMUP32b
CTGTGTCATCGAGAGACATTCAGAGACAATCTTGGGTCTGACCCAAAATCTCGAATTCGCTAAACAGGCACGTTACCGATGTATACGGGGGGATGAAGCGCCCCCCCGAATACATCGCCGTGCCAAAGGGGAAACCCCTTTGAAACCCCGCAGAGCGTTAGCGCTGCGCTTGTTGTTAGTCATCAGTCAGTAAATCAGTCGCGTCATTCATCGTCGGAGGC
CTGTGTCATCGAGAGACATTCAGAGACAATCTTGGGTCTGACCCAAAATCTCGAATTCGCTAAACAGGCACGTTACCGATGTATACGGGGGGATGAAGCGCCCCCCCGAATACATCGCCGTGCCAAAGGGGAAACCCCTTTGAAACCCCGCAGAGCGTTAGCGCTGCGCTTGTTGTTAGTCATCAGTCAGTAAATCAGTCGCGTCATTCATCGTCGGAGGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 4078 | GenBank | WP_266008294 |
| Name | t4cp2_OQB64_RS26865_pMUP32b |
UniProt ID | _ |
| Length | 557 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 557 a.a. Molecular weight: 61653.29 Da Isoelectric Point: 6.6026
>WP_266008294.1 type IV secretory system conjugative DNA transfer family protein [Pseudomonas syringae]
MAKAKSKPISPDNPQERIEEHPAILIGKHPTKDTFLASYGQTFVMLAAPPGTGKTVGVVTPNLLSYPDSV
VVNDPKFENWRDTAGFRAAAGHKVYRFSPELLETHRWNPLSALSRDPLYRLGQIRTLAGVLFVSDNPKNQ
EWYNKAANVFAAILLYLMEMEGMKLNGMKLTLPQAYEVASLGTGLGVWAQQAIEQHSTGPNALSVETLRE
LNGVFEASKNKSSGWSTTVDILRGALSMYAEKTVAWAVSDTDIDFTKLRKEKISIYFCVTGNAIKKYGPL
MNLFFTQAIQQNTDVMPEQGGHCADGTLILKYQVLFVIDEIAVMGRIEIMEMAPALTRGAGLRYIIIFQG
KDQLRSERTYGREAGNGIMQAFHIEIVYGTGNVELAKEYSTRLGNTTVRVHNQSLNRQKHEIGARGQTDS
YSEQPRPLLLPQEVSALPYGEELIFVEATKTTPAANIRARKIFWYEEPVFKERAGMPTPDVPSGDASKID
ALTVPVRTVKAKVAVADTKPMQAEQRQRWNPKEKAANAVPDQPDAAQPVDVEPDLEPAQTDDTSEPM
MAKAKSKPISPDNPQERIEEHPAILIGKHPTKDTFLASYGQTFVMLAAPPGTGKTVGVVTPNLLSYPDSV
VVNDPKFENWRDTAGFRAAAGHKVYRFSPELLETHRWNPLSALSRDPLYRLGQIRTLAGVLFVSDNPKNQ
EWYNKAANVFAAILLYLMEMEGMKLNGMKLTLPQAYEVASLGTGLGVWAQQAIEQHSTGPNALSVETLRE
LNGVFEASKNKSSGWSTTVDILRGALSMYAEKTVAWAVSDTDIDFTKLRKEKISIYFCVTGNAIKKYGPL
MNLFFTQAIQQNTDVMPEQGGHCADGTLILKYQVLFVIDEIAVMGRIEIMEMAPALTRGAGLRYIIIFQG
KDQLRSERTYGREAGNGIMQAFHIEIVYGTGNVELAKEYSTRLGNTTVRVHNQSLNRQKHEIGARGQTDS
YSEQPRPLLLPQEVSALPYGEELIFVEATKTTPAANIRARKIFWYEEPVFKERAGMPTPDVPSGDASKID
ALTVPVRTVKAKVAVADTKPMQAEQRQRWNPKEKAANAVPDQPDAAQPVDVEPDLEPAQTDDTSEPM
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 25330..35684
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OQB64_RS26735 (OQB64_26735) | 20439..20624 | - | 186 | WP_005730485 | hypothetical protein | - |
| OQB64_RS26740 (OQB64_26740) | 20675..20953 | - | 279 | WP_003320513 | hypothetical protein | - |
| OQB64_RS26745 (OQB64_26745) | 21046..21177 | - | 132 | Protein_25 | helix-turn-helix domain-containing protein | - |
| OQB64_RS26750 (OQB64_26750) | 21611..21937 | - | 327 | WP_003319696 | type II toxin-antitoxin system PemK/MazF family toxin | - |
| OQB64_RS26755 (OQB64_26755) | 21934..22158 | - | 225 | WP_003319697 | antitoxin MazE family protein | - |
| OQB64_RS26760 (OQB64_26760) | 22648..23280 | + | 633 | WP_003381804 | ParA family protein | - |
| OQB64_RS26765 (OQB64_26765) | 23316..23570 | + | 255 | WP_003381801 | hypothetical protein | - |
| OQB64_RS26770 (OQB64_26770) | 23995..24660 | - | 666 | WP_024647998 | LexA family transcriptional regulator | - |
| OQB64_RS26775 (OQB64_26775) | 24748..24978 | + | 231 | WP_010215521 | Cro/CI family transcriptional regulator | - |
| OQB64_RS26780 (OQB64_26780) | 25330..26028 | + | 699 | WP_024650160 | lytic transglycosylase domain-containing protein | virB1 |
| OQB64_RS26785 (OQB64_26785) | 26028..26363 | + | 336 | WP_024650161 | hypothetical protein | - |
| OQB64_RS26790 (OQB64_26790) | 26376..26651 | + | 276 | WP_024650162 | VirB3 family type IV secretion system protein | virB3 |
| OQB64_RS26795 (OQB64_26795) | 26712..29264 | + | 2553 | WP_024650163 | VirB4 family type IV secretion system protein | virb4 |
| OQB64_RS26800 (OQB64_26800) | 29261..29947 | + | 687 | WP_024650164 | P-type DNA transfer protein VirB5 | - |
| OQB64_RS26805 (OQB64_26805) | 29980..30333 | + | 354 | WP_024650165 | hypothetical protein | - |
| OQB64_RS26810 (OQB64_26810) | 30344..31276 | + | 933 | WP_024650166 | type IV secretion system protein | virB6 |
| OQB64_RS26815 (OQB64_26815) | 31344..31658 | + | 315 | WP_080271125 | conjugal transfer protein | - |
| OQB64_RS26820 (OQB64_26820) | 31696..32484 | + | 789 | WP_024650167 | type IV secretion system protein | virB8 |
| OQB64_RS26825 (OQB64_26825) | 32474..33283 | + | 810 | WP_024650168 | P-type conjugative transfer protein VirB9 | virB9 |
| OQB64_RS26830 (OQB64_26830) | 33270..34619 | + | 1350 | WP_024650169 | TrbI/VirB10 family protein | virB10 |
| OQB64_RS26835 (OQB64_26835) | 34629..35684 | + | 1056 | WP_024650170 | P-type DNA transfer ATPase VirB11 | virB11 |
| OQB64_RS26840 (OQB64_26840) | 35698..36084 | + | 387 | WP_003347401 | hypothetical protein | - |
| OQB64_RS26845 (OQB64_26845) | 36069..36425 | + | 357 | WP_024650171 | hypothetical protein | - |
| OQB64_RS26850 (OQB64_26850) | 36702..37325 | + | 624 | WP_003347396 | glycoside hydrolase family 25 protein | - |
| OQB64_RS26855 (OQB64_26855) | 37660..40080 | + | 2421 | WP_116894210 | ATP-binding protein | - |
| OQB64_RS26860 (OQB64_26860) | 40192..40425 | + | 234 | WP_003347392 | hypothetical protein | - |
Host bacterium
| ID | 6489 | GenBank | NZ_CP110811 |
| Plasmid name | pMUP32b | Incompatibility group | - |
| Plasmid size | 54993 bp | Coordinate of oriT [Strand] | 47783..48003 [+] |
| Host baterium | Pseudomonas syringae strain MUP32 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |