Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106051 |
| Name | oriT_pMUP32a  |
| Organism | Pseudomonas syringae strain MUP32 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP110810 (56755..56975 [+], 221 nt) |
| oriT length | 221 nt |
| IRs (inverted repeats) | 124..131, 135..142 (CAAAGGGG..CCCCTTTG) 86..92, 102..108 (CGGGGGG..CCCCCCG) 87..92, 101..106 (GGGGGG..CCCCCC) 32..38, 40..46 (TTGGGTC..GACCCAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 221 nt
>oriT_pMUP32a
CTGTGTCATCGAGAGACATTCAGAGACAATCTTGGGTCTGACCCAAAATCTCGAATTCGCTAAACAGGCACGTTACCGATGTATACGGGGGGATGAAGCGCCCCCCCGAATACATCGCCGTGCCAAAGGGGAAACCCCTTTGAAACCCCGCAGAGCGTTAGCGCTGCGCTTGTTGTTAGTCATCAGTCAGTAAATCAGTCGCGTCATTCATCGTCGGAGGC
CTGTGTCATCGAGAGACATTCAGAGACAATCTTGGGTCTGACCCAAAATCTCGAATTCGCTAAACAGGCACGTTACCGATGTATACGGGGGGATGAAGCGCCCCCCCGAATACATCGCCGTGCCAAAGGGGAAACCCCTTTGAAACCCCGCAGAGCGTTAGCGCTGCGCTTGTTGTTAGTCATCAGTCAGTAAATCAGTCGCGTCATTCATCGTCGGAGGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 4077 | GenBank | WP_266008243 |
| Name | t4cp2_OQB64_RS26560_pMUP32a |
UniProt ID | _ |
| Length | 557 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 557 a.a. Molecular weight: 61654.24 Da Isoelectric Point: 6.2657
>WP_266008243.1 type IV secretory system conjugative DNA transfer family protein [Pseudomonas syringae]
MGKAKSKPISPDNPQERIEEHPAILIGKHPTKDTFLASYGQTFVMLAAPPGTGKTVGVVTPNLLSYPDSV
VVNDPKFENWRDTAGFRAAAGHKVYRFSPELLETHRWNPLSALSRDPLYRLGQIRTLAGVLFVSDNPKNQ
EWYNKAANVFAAILLYLMEMEGMKLNGMKLTLPQAYEVASLGTGLGVWAQQAIEQHSTGPNALSVETLRE
LNGVFEASKNKSSGWSTTVDILRGALSMYAEKTVAWAVSDTDIDFTKLRKEKISIYFCVTGNAIKKYGPL
MNLFFTQAIQQNTDVMPEQGGHCADGTLILKYQVLFVIDEIAVMGRIEIMEMAPALTRGAGLRYIIIFQG
KDQLRSERTYGREAGNGIMQAFHIEIVYGTGNVELAKEYSTRLGNTTVRVHNQSLNRQKHEIGARGQTDS
YSEQPRPLLLPQEVSALPYGEELIFVEATKTTPAANIRARKIFWYEEPVFKERAGMPTPDVPSGDASKID
ALTVPVRTVEAKVAVADTKPMQAEQRQRWNPKEKAANAVPDQPDAAQPVDVEPDLEPAQTDDTPETM
MGKAKSKPISPDNPQERIEEHPAILIGKHPTKDTFLASYGQTFVMLAAPPGTGKTVGVVTPNLLSYPDSV
VVNDPKFENWRDTAGFRAAAGHKVYRFSPELLETHRWNPLSALSRDPLYRLGQIRTLAGVLFVSDNPKNQ
EWYNKAANVFAAILLYLMEMEGMKLNGMKLTLPQAYEVASLGTGLGVWAQQAIEQHSTGPNALSVETLRE
LNGVFEASKNKSSGWSTTVDILRGALSMYAEKTVAWAVSDTDIDFTKLRKEKISIYFCVTGNAIKKYGPL
MNLFFTQAIQQNTDVMPEQGGHCADGTLILKYQVLFVIDEIAVMGRIEIMEMAPALTRGAGLRYIIIFQG
KDQLRSERTYGREAGNGIMQAFHIEIVYGTGNVELAKEYSTRLGNTTVRVHNQSLNRQKHEIGARGQTDS
YSEQPRPLLLPQEVSALPYGEELIFVEATKTTPAANIRARKIFWYEEPVFKERAGMPTPDVPSGDASKID
ALTVPVRTVEAKVAVADTKPMQAEQRQRWNPKEKAANAVPDQPDAAQPVDVEPDLEPAQTDDTPETM
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 34159..44513
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OQB64_RS26445 (OQB64_26445) | 30548..31186 | - | 639 | WP_024647993 | recombinase family protein | - |
| OQB64_RS26450 (OQB64_26450) | 31477..32109 | + | 633 | WP_003381804 | ParA family protein | - |
| OQB64_RS26455 (OQB64_26455) | 32145..32399 | + | 255 | WP_003381801 | hypothetical protein | - |
| OQB64_RS26460 (OQB64_26460) | 32824..33489 | - | 666 | WP_024647998 | LexA family transcriptional regulator | - |
| OQB64_RS26465 (OQB64_26465) | 33577..33807 | + | 231 | WP_010215521 | Cro/CI family transcriptional regulator | - |
| OQB64_RS26470 (OQB64_26470) | 34159..34857 | + | 699 | WP_024650160 | lytic transglycosylase domain-containing protein | virB1 |
| OQB64_RS26475 (OQB64_26475) | 34857..35192 | + | 336 | WP_024650161 | hypothetical protein | - |
| OQB64_RS26480 (OQB64_26480) | 35205..35480 | + | 276 | WP_024650162 | VirB3 family type IV secretion system protein | virB3 |
| OQB64_RS26485 (OQB64_26485) | 35541..38093 | + | 2553 | WP_024650163 | VirB4 family type IV secretion system protein | virb4 |
| OQB64_RS26490 (OQB64_26490) | 38090..38776 | + | 687 | WP_024650164 | P-type DNA transfer protein VirB5 | - |
| OQB64_RS26495 (OQB64_26495) | 38809..39162 | + | 354 | WP_024650165 | hypothetical protein | - |
| OQB64_RS26500 (OQB64_26500) | 39173..40105 | + | 933 | WP_024650166 | type IV secretion system protein | virB6 |
| OQB64_RS26505 (OQB64_26505) | 40173..40487 | + | 315 | WP_080271125 | conjugal transfer protein | - |
| OQB64_RS26510 (OQB64_26510) | 40525..41313 | + | 789 | WP_024650167 | type IV secretion system protein | virB8 |
| OQB64_RS26515 (OQB64_26515) | 41303..42112 | + | 810 | WP_024650168 | P-type conjugative transfer protein VirB9 | virB9 |
| OQB64_RS26520 (OQB64_26520) | 42099..43448 | + | 1350 | WP_024650169 | TrbI/VirB10 family protein | virB10 |
| OQB64_RS26525 (OQB64_26525) | 43458..44513 | + | 1056 | WP_024650170 | P-type DNA transfer ATPase VirB11 | virB11 |
| OQB64_RS26530 (OQB64_26530) | 44527..44913 | + | 387 | WP_003347401 | hypothetical protein | - |
| OQB64_RS26535 (OQB64_26535) | 44898..45254 | + | 357 | WP_024650171 | hypothetical protein | - |
| OQB64_RS26540 (OQB64_26540) | 45531..46154 | + | 624 | WP_003347396 | glycoside hydrolase family 25 protein | - |
| OQB64_RS26545 (OQB64_26545) | 46489..48543 | + | 2055 | WP_032632215 | ATP-binding protein | - |
| OQB64_RS26550 (OQB64_26550) | 48545..49054 | + | 510 | WP_241197533 | hypothetical protein | - |
| OQB64_RS26555 (OQB64_26555) | 49166..49399 | + | 234 | WP_266008241 | hypothetical protein | - |
Host bacterium
| ID | 6488 | GenBank | NZ_CP110810 |
| Plasmid name | pMUP32a | Incompatibility group | - |
| Plasmid size | 61675 bp | Coordinate of oriT [Strand] | 56755..56975 [+] |
| Host baterium | Pseudomonas syringae strain MUP32 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |