Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106027 |
| Name | oriT_p65L  |
| Organism | Klebsiella pneumoniae strain Kp11-81 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP082157 (58840..58945 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_p65L
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4053 | GenBank | WP_004187323 |
| Name | Relaxase_K6977_RS28100_p65L |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 4069 | GenBank | WP_004206885 |
| Name | t4cp2_K6977_RS27800_p65L |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1873..23585
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K6977_RS27710 (K6977_27640) | 33..1907 | + | 1875 | WP_226079076 | LPD7 domain-containing protein | - |
| K6977_RS27715 (K6977_27645) | 1873..2385 | + | 513 | WP_011091071 | hypothetical protein | traL |
| K6977_RS27720 (K6977_27650) | 2386..2598 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| K6977_RS27725 (K6977_27655) | 2570..2980 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| K6977_RS27730 (K6977_27660) | 3048..3761 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| K6977_RS27735 (K6977_27665) | 3770..4921 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| K6977_RS27740 (K6977_27670) | 4933..6282 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| K6977_RS27745 (K6977_27675) | 6294..6998 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| K6977_RS27750 (K6977_27680) | 7022..7552 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| K6977_RS27755 (K6977_27685) | 7569..7958 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| K6977_RS27760 (K6977_27690) | 8004..8498 | + | 495 | WP_004187480 | hypothetical protein | - |
| K6977_RS27765 (K6977_27695) | 8495..11545 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| K6977_RS27770 (K6977_27700) | 11542..12750 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| K6977_RS27775 (K6977_27705) | 12747..13343 | + | 597 | WP_015060003 | hypothetical protein | - |
| K6977_RS27780 (K6977_27710) | 13336..15531 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| K6977_RS27785 (K6977_27715) | 15533..16186 | + | 654 | WP_015060005 | hypothetical protein | - |
| K6977_RS27790 (K6977_27720) | 16267..16497 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
| K6977_RS27795 (K6977_27725) | 16793..17848 | + | 1056 | WP_015060006 | plasmid replication initiator RepA | - |
| K6977_RS28170 | 19071..19166 | + | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| K6977_RS27800 (K6977_27730) | 19217..21304 | - | 2088 | WP_004206885 | conjugal transfer protein TrbC | - |
| K6977_RS27805 (K6977_27735) | 21317..22267 | - | 951 | WP_004206886 | DsbC family protein | trbB |
| K6977_RS27810 (K6977_27740) | 22278..23585 | - | 1308 | WP_015059988 | hypothetical protein | trbA |
| K6977_RS27815 (K6977_27745) | 23585..23980 | - | 396 | WP_004187436 | lytic transglycosylase domain-containing protein | - |
| K6977_RS27820 (K6977_27750) | 24085..24468 | - | 384 | WP_019725042 | DUF1496 domain-containing protein | - |
| K6977_RS27825 (K6977_27755) | 24548..24982 | + | 435 | Protein_25 | CPBP family intramembrane glutamate endopeptidase | - |
| K6977_RS27830 (K6977_27760) | 24997..26202 | - | 1206 | WP_025987686 | IS4-like element IS10A family transposase | - |
| K6977_RS27840 (K6977_27770) | 27115..27912 | + | 798 | WP_015059991 | OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-48 | - |
Host bacterium
| ID | 6464 | GenBank | NZ_CP082157 |
| Plasmid name | p65L | Incompatibility group | IncL/M |
| Plasmid size | 65599 bp | Coordinate of oriT [Strand] | 58840..58945 [+] |
| Host baterium | Klebsiella pneumoniae strain Kp11-81 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA8 |