Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106013 |
| Name | oriT_pKpn14-4  |
| Organism | Klebsiella pneumoniae strain Kpn-14 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP047704 (49698..49802 [+], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_pKpn14-4
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4045 | GenBank | WP_004206924 |
| Name | Relaxase_GVI79_RS30175_pKpn14-4 |
UniProt ID | A0A2V4FJ40 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2V4FJ40 |
T4CP
| ID | 4058 | GenBank | WP_004206885 |
| Name | t4cp2_GVI79_RS29845_pKpn14-4 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 52600..71796
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GVI79_RS30155 (GVI79_29760) | 47684..47995 | + | 312 | WP_004187333 | hypothetical protein | - |
| GVI79_RS30160 (GVI79_29765) | 48128..48664 | + | 537 | WP_036970776 | hypothetical protein | - |
| GVI79_RS30165 (GVI79_29770) | 49165..49530 | - | 366 | WP_015586046 | hypothetical protein | - |
| GVI79_RS30170 (GVI79_29775) | 49806..50123 | + | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| GVI79_RS30175 (GVI79_29780) | 50110..52089 | + | 1980 | WP_004206924 | TraI/MobA(P) family conjugative relaxase | - |
| GVI79_RS30180 (GVI79_29785) | 52103..52603 | + | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| GVI79_RS30185 (GVI79_29790) | 52600..53379 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| GVI79_RS30190 (GVI79_29795) | 53390..54553 | + | 1164 | WP_004206926 | plasmid transfer ATPase TraJ | virB11 |
| GVI79_RS30195 (GVI79_29800) | 54543..54803 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| GVI79_RS30665 | 54828..58133 | + | 3306 | WP_220381329 | LPD7 domain-containing protein | - |
| GVI79_RS30205 (GVI79_29810) | 58099..58611 | + | 513 | WP_011091071 | hypothetical protein | traL |
| GVI79_RS30210 (GVI79_29815) | 58612..58824 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| GVI79_RS30215 (GVI79_29820) | 58796..59206 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| GVI79_RS30220 (GVI79_29825) | 59274..59987 | + | 714 | WP_223294958 | DotI/IcmL family type IV secretion protein | traM |
| GVI79_RS30225 (GVI79_29830) | 59996..61147 | + | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| GVI79_RS30230 (GVI79_29835) | 61159..62508 | + | 1350 | WP_036970781 | conjugal transfer protein TraO | traO |
| GVI79_RS30235 (GVI79_29840) | 62520..63224 | + | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| GVI79_RS30240 (GVI79_29845) | 63248..63778 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| GVI79_RS30245 (GVI79_29850) | 63795..64184 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| GVI79_RS30250 (GVI79_29855) | 64230..64724 | + | 495 | WP_004187480 | hypothetical protein | - |
| GVI79_RS30255 (GVI79_29860) | 64721..67771 | + | 3051 | WP_011154474 | conjugative transfer protein | traU |
| GVI79_RS30260 (GVI79_29865) | 67768..68976 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| GVI79_RS30265 (GVI79_29870) | 68973..69623 | + | 651 | WP_004187488 | hypothetical protein | - |
| GVI79_RS30270 (GVI79_29875) | 69616..71796 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| GVI79_RS30275 (GVI79_29880) | 71799..72452 | + | 654 | WP_004206935 | hypothetical protein | - |
| GVI79_RS30280 (GVI79_29885) | 72526..72756 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 6450 | GenBank | NZ_CP047704 |
| Plasmid name | pKpn14-4 | Incompatibility group | IncL/M |
| Plasmid size | 73052 bp | Coordinate of oriT [Strand] | 49698..49802 [+] |
| Host baterium | Klebsiella pneumoniae strain Kpn-14 |
Cargo genes
| Drug resistance gene | qnrS1, blaCTX-M-14b, aph(3'')-Ib, aph(3')-VIb, aph(6)-Id |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |