Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106003
Name   oriT_pEclE4-1 in_silico
Organism   Enterobacter hormaechei strain Eho-E4
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP048697 (209243..209497 [-], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_pEclE4-1
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4036 GenBank   WP_012477394
Name   mobH_GVI76_RS25885_pEclE4-1 insolico UniProt ID   A0A2Z2E7B6
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118067.47 Da        Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A2Z2E7B6


T4CP

ID   4048 GenBank   WP_237902866
Name   traD_GVI76_RS25890_pEclE4-1 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69892.11 Da        Isoelectric Point: 7.9672

>WP_237902866.1 conjugative transfer system coupling protein TraD [Enterobacter hormaechei]
MAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHADRAY
QIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTGKTV
LQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSDLTS
RLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARYYGY
DVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIMPVF
DMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGSRYN
AQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLCNNY
ISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQVPMF
HIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(126-176)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(333-536)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2429..11636

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI76_RS24815 (GVI76_24810) 1..876 + 876 WP_000594611 RepB family plasmid replication initiator protein -
GVI76_RS24820 (GVI76_24815) 2025..2378 + 354 WP_000423602 hypothetical protein -
GVI76_RS24825 (GVI76_24820) 2396..2746 + 351 WP_001447770 type IV conjugative transfer system protein TraL -
GVI76_RS24830 (GVI76_24825) 2758..3546 + 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
GVI76_RS24835 (GVI76_24830) 3546..4817 + 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
GVI76_RS24840 (GVI76_24835) 4819..5280 + 462 WP_021242895 plasmid transfer protein HtdO -
GVI76_RS24845 (GVI76_24840) 5270..6625 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
GVI76_RS24850 (GVI76_24845) 6633..7115 + 483 WP_000377632 hypothetical protein -
GVI76_RS24855 (GVI76_24850) 7234..7986 + 753 WP_182911723 protein-disulfide isomerase HtdT -
GVI76_RS24860 (GVI76_24855) 7996..8946 + 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
GVI76_RS24865 (GVI76_24860) 8955..11636 + 2682 WP_000387412 TraC family protein virb4
GVI76_RS24870 14185..14346 + 162 WP_001371949 hypothetical protein -

Host bacterium

ID   6440 GenBank   NZ_CP048697
Plasmid name   pEclE4-1 Incompatibility group   IncHI2A
Plasmid size   331873 bp Coordinate of oriT [Strand]   209243..209497 [-]
Host baterium   Enterobacter hormaechei strain Eho-E4

Cargo genes

Drug resistance gene   blaACC-1, sul1, aph(6)-Id, aph(3'')-Ib, mcr-9, qacE
Virulence gene   -
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE, silE, silS, silR, silC, silF, silB, silA, silP, pcoS, pcoE, rcnR/yohL, arsC, arsB, arsR, arsH, merR, merT, merP, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -