Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105999
Name   oriT_pEclE2-1 in_silico
Organism   Enterobacter hormaechei strain Eho-E2
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP047716 (55909..55995 [-], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_pEclE2-1
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4034 GenBank   WP_022650008
Name   Relaxase_GVI74_RS23705_pEclE2-1 insolico UniProt ID   A0A4Q2QTK9
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102314.37 Da        Isoelectric Point: 8.2756

>WP_022650008.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(51-317)


  Protein structure


Source ID Structure
AlphaFold DB A0A4Q2QTK9


T4CP

ID   4046 GenBank   WP_022650006
Name   trbC_GVI74_RS23720_pEclE2-1 insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87660.94 Da        Isoelectric Point: 6.8660

>WP_022650006.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 62118..96562

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI74_RS23710 (GVI74_23655) 59134..59337 - 204 WP_123839396 hypothetical protein -
GVI74_RS23715 (GVI74_23660) 59471..59740 - 270 WP_022650007 hypothetical protein -
GVI74_RS23720 (GVI74_23665) 59814..62114 - 2301 WP_022650006 F-type conjugative transfer protein TrbC -
GVI74_RS23725 (GVI74_23670) 62118..63374 - 1257 WP_126530067 hypothetical protein trbB
GVI74_RS23730 (GVI74_23675) 63355..64563 - 1209 WP_022650004 hypothetical protein trbA
GVI74_RS23735 (GVI74_23680) 64884..65516 - 633 WP_022650003 plasmid IncI1-type surface exclusion protein ExcA -
GVI74_RS23740 (GVI74_23685) 65578..67737 - 2160 WP_022650002 DotA/TraY family protein traY
GVI74_RS23745 (GVI74_23690) 67795..68337 - 543 WP_022650001 conjugal transfer protein TraX -
GVI74_RS23750 (GVI74_23695) 68334..69536 - 1203 WP_022650000 conjugal transfer protein TraW traW
GVI74_RS23755 (GVI74_23700) 69549..70118 - 570 WP_022649999 hypothetical protein traV
GVI74_RS23760 (GVI74_23705) 70118..73171 - 3054 WP_022649998 hypothetical protein traU
GVI74_RS23765 (GVI74_23710) 73258..73983 - 726 WP_063864836 hypothetical protein traT
GVI74_RS23770 (GVI74_23715) 74167..74571 - 405 WP_022649996 DUF6750 family protein traR
GVI74_RS23775 (GVI74_23720) 74606..75130 - 525 WP_022649995 conjugal transfer protein TraQ traQ
GVI74_RS23780 (GVI74_23725) 75130..75903 - 774 WP_123839398 conjugal transfer protein TraP traP
GVI74_RS23785 (GVI74_23730) 75884..77191 - 1308 WP_022649993 conjugal transfer protein TraO traO
GVI74_RS23790 (GVI74_23735) 77191..78171 - 981 WP_022649992 DotH/IcmK family type IV secretion protein traN
GVI74_RS23795 (GVI74_23740) 78182..78799 - 618 WP_179297403 DotI/IcmL family type IV secretion protein traM
GVI74_RS23800 (GVI74_23745) 78889..79239 - 351 WP_022649990 hypothetical protein traL
GVI74_RS23805 (GVI74_23750) 79257..82799 - 3543 WP_032635082 LPD7 domain-containing protein -
GVI74_RS23810 (GVI74_23755) 82871..83158 - 288 WP_022649988 hypothetical protein traK
GVI74_RS23815 (GVI74_23760) 83155..84315 - 1161 WP_022649987 plasmid transfer ATPase TraJ virB11
GVI74_RS23820 (GVI74_23765) 84328..85134 - 807 WP_022649986 type IV secretory system conjugative DNA transfer family protein traI
GVI74_RS23825 (GVI74_23770) 85131..85604 - 474 WP_022649985 DotD/TraH family lipoprotein -
GVI74_RS23830 (GVI74_23775) 85675..86091 - 417 WP_123839394 hypothetical protein -
GVI74_RS23835 (GVI74_23780) 86200..87405 - 1206 WP_022649984 conjugal transfer protein TraF -
GVI74_RS23840 (GVI74_23785) 87511..88347 - 837 WP_022649983 hypothetical protein traE
GVI74_RS23845 (GVI74_23790) 88494..88970 - 477 WP_022649982 hypothetical protein -
GVI74_RS23850 (GVI74_23795) 88990..90150 - 1161 WP_022649981 site-specific integrase -
GVI74_RS23855 (GVI74_23800) 90728..92065 - 1338 WP_040110252 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
GVI74_RS23860 (GVI74_23805) 92062..92736 - 675 WP_022649979 prepilin peptidase -
GVI74_RS23865 (GVI74_23810) 92721..93203 - 483 WP_045261878 lytic transglycosylase domain-containing protein virB1
GVI74_RS23870 (GVI74_23815) 93288..93902 - 615 WP_022649977 type 4 pilus major pilin -
GVI74_RS23875 (GVI74_23820) 93916..95010 - 1095 WP_032623575 type II secretion system F family protein -
GVI74_RS23880 (GVI74_23825) 95003..96562 - 1560 WP_022649975 ATPase, T2SS/T4P/T4SS family virB11
GVI74_RS23885 (GVI74_23830) 96565..97080 - 516 WP_022649974 type IV pilus biogenesis protein PilP -
GVI74_RS23890 (GVI74_23835) 97064..98368 - 1305 WP_022649973 type 4b pilus protein PilO2 -
GVI74_RS23895 (GVI74_23840) 98370..100046 - 1677 WP_022649972 PilN family type IVB pilus formation outer membrane protein -
GVI74_RS23900 (GVI74_23845) 100061..100498 - 438 WP_022649971 type IV pilus biogenesis protein PilM -

Host bacterium

ID   6436 GenBank   NZ_CP047716
Plasmid name   pEclE2-1 Incompatibility group   -
Plasmid size   106697 bp Coordinate of oriT [Strand]   55909..55995 [-]
Host baterium   Enterobacter hormaechei strain Eho-E2

Cargo genes

Drug resistance gene   blaLAP-2, qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIC1, AcrIIA7