Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105938 |
| Name | oriT_p3-va18651  |
| Organism | Enterobacter hormaechei subsp. xiangfangensis strain va18651 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP097345 (35486..35591 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_p3-va18651
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4005 | GenBank | WP_004187323 |
| Name | Relaxase_M5K26_RS25340_p3-va18651 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 4011 | GenBank | WP_004206885 |
| Name | t4cp2_M5K26_RS25455_p3-va18651 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 38387..57676
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| M5K26_RS25320 (M5K26_25290) | 33471..33782 | + | 312 | WP_004187333 | hypothetical protein | - |
| M5K26_RS25325 (M5K26_25295) | 33915..34451 | + | 537 | WP_004187332 | hypothetical protein | - |
| M5K26_RS25330 (M5K26_25300) | 34953..35348 | - | 396 | WP_019725163 | hypothetical protein | - |
| M5K26_RS25335 (M5K26_25305) | 35383..35910 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| M5K26_RS25340 (M5K26_25310) | 35897..37876 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| M5K26_RS25345 (M5K26_25315) | 37890..38390 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| M5K26_RS25350 (M5K26_25320) | 38387..39166 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| M5K26_RS25355 (M5K26_25325) | 39177..40340 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| M5K26_RS25360 (M5K26_25330) | 40330..40590 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| M5K26_RS25365 (M5K26_25335) | 40615..44052 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| M5K26_RS25370 (M5K26_25340) | 44018..44530 | + | 513 | WP_011091071 | hypothetical protein | traL |
| M5K26_RS25375 (M5K26_25345) | 44531..44743 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| M5K26_RS25380 (M5K26_25350) | 44715..45125 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| M5K26_RS25385 (M5K26_25355) | 45193..45906 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| M5K26_RS25390 (M5K26_25360) | 45915..47066 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| M5K26_RS25395 (M5K26_25365) | 47078..48427 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| M5K26_RS25400 (M5K26_25370) | 48439..49143 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| M5K26_RS25405 (M5K26_25375) | 49167..49697 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| M5K26_RS25410 (M5K26_25380) | 49714..50103 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| M5K26_RS25415 (M5K26_25385) | 50149..50643 | + | 495 | WP_004187480 | hypothetical protein | - |
| M5K26_RS25420 (M5K26_25390) | 50640..53690 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| M5K26_RS25425 (M5K26_25395) | 53687..54895 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| M5K26_RS25430 (M5K26_25400) | 54892..55488 | + | 597 | WP_015060003 | hypothetical protein | - |
| M5K26_RS25435 (M5K26_25405) | 55481..57676 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| M5K26_RS25440 (M5K26_25410) | 57678..58331 | + | 654 | WP_015060005 | hypothetical protein | - |
| M5K26_RS25445 (M5K26_25415) | 58412..58642 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
| M5K26_RS25450 (M5K26_25420) | 58938..59993 | + | 1056 | WP_015060006 | plasmid replication initiator RepA | - |
| M5K26_RS26290 | 61216..61311 | + | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
Host bacterium
| ID | 6375 | GenBank | NZ_CP097345 |
| Plasmid name | p3-va18651 | Incompatibility group | IncL/M |
| Plasmid size | 63589 bp | Coordinate of oriT [Strand] | 35486..35591 [+] |
| Host baterium | Enterobacter hormaechei subsp. xiangfangensis strain va18651 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |