Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105923 |
| Name | oriT_CLK 101|unnamed1  |
| Organism | Lacticaseibacillus rhamnosus strain CLK 101 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP101644 (2528..2547 [-], 20 nt) |
| oriT length | 20 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 20 nt
>oriT_CLK 101|unnamed1
GTTGTGTGTAAGTGCGCATT
GTTGTGTGTAAGTGCGCATT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3996 | GenBank | WP_064522591 |
| Name | mobQ_NOS86_RS13590_CLK 101|unnamed1 |
UniProt ID | _ |
| Length | 687 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 687 a.a. Molecular weight: 80588.08 Da Isoelectric Point: 9.3620
>WP_064522591.1 MobQ family relaxase [Lacticaseibacillus rhamnosus]
MAIFHMSFSNISAGKGRSAIAGASYRSGEKLFDQKEGRSYFYARSVMPESFILTPNNAPEWASDREKLWN
EVERKDRRANSRYAKEFNVALPVELSEDEQKELLTKYVQENFVDEGMVADVAIHRDHPDNPHAHVMLTNR
PFNPDGTWGLKSKRKNILDENGNKTYIGNSRFPRSRKVWLVDWDKKEKINQWRHNWAVSVNQVLEQKNIP
DRISEKSFEDQGINEVPTQHEGINSKRHERNKFNQQVKNYRKSKAGYKNNQEKVINRGHLDSLSKHFSFN
EKKVVKELSHELKTYISLESLDDKRRMLFNWKNSTLIKHAVGEDVTKQLLTINQQESSLKKADELLNKVV
DRTTKKLYPELDFEQTTAAERRELIKETDSEQTVFKGSELNERLMNIRDDLLTQQLLTFTKRPYVGFKLL
MQQEKEVKIELKYTLMIHDDSLESLEHVDQGLLEKYSPTEQQKITRAVKDLRTIMAVKQVIKTQYHEVLK
RAFPKGDLDELPMTKQEQAYTAVMYYDPVLKPCQAETIEQWQANPPQVFSPQEHQQGLAYLSGQLSLDQL
ENHHLQRVLKHDGTKQLFFGECKADPTIKNSQIEKIQMQLKEQQAKDDQYRKANIGHYQPLNYKPVSPSY
YLKTAFSDAIMTVLYARDEDYQRQQQLRGLKETEWEMTKKQRQHQTRNRHENGGMHL
MAIFHMSFSNISAGKGRSAIAGASYRSGEKLFDQKEGRSYFYARSVMPESFILTPNNAPEWASDREKLWN
EVERKDRRANSRYAKEFNVALPVELSEDEQKELLTKYVQENFVDEGMVADVAIHRDHPDNPHAHVMLTNR
PFNPDGTWGLKSKRKNILDENGNKTYIGNSRFPRSRKVWLVDWDKKEKINQWRHNWAVSVNQVLEQKNIP
DRISEKSFEDQGINEVPTQHEGINSKRHERNKFNQQVKNYRKSKAGYKNNQEKVINRGHLDSLSKHFSFN
EKKVVKELSHELKTYISLESLDDKRRMLFNWKNSTLIKHAVGEDVTKQLLTINQQESSLKKADELLNKVV
DRTTKKLYPELDFEQTTAAERRELIKETDSEQTVFKGSELNERLMNIRDDLLTQQLLTFTKRPYVGFKLL
MQQEKEVKIELKYTLMIHDDSLESLEHVDQGLLEKYSPTEQQKITRAVKDLRTIMAVKQVIKTQYHEVLK
RAFPKGDLDELPMTKQEQAYTAVMYYDPVLKPCQAETIEQWQANPPQVFSPQEHQQGLAYLSGQLSLDQL
ENHHLQRVLKHDGTKQLFFGECKADPTIKNSQIEKIQMQLKEQQAKDDQYRKANIGHYQPLNYKPVSPSY
YLKTAFSDAIMTVLYARDEDYQRQQQLRGLKETEWEMTKKQRQHQTRNRHENGGMHL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 4001 | GenBank | WP_064522582 |
| Name | t4cp2_NOS86_RS13655_CLK 101|unnamed1 |
UniProt ID | _ |
| Length | 517 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 517 a.a. Molecular weight: 58253.48 Da Isoelectric Point: 5.0525
>WP_064522582.1 VirD4-like conjugal transfer protein, CD1115 family [Lacticaseibacillus rhamnosus]
MNGTILGIVDKRIVYQNNSTKPNRNIFVVGGPGSYKTQSVVITNLFNETENSIVVTDPKGELYEKTAGIK
LAQGYQVHVVNFANMAHSDRYNPFDYIQRDIQAETVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMN
HRPPEQRNLAGVTQVLQENDVEAEEKGADSPLDTLFLDLTMTDPARRAYELGFKKAKGEMKASIIESLLA
TISKFVDKEVANFTSFSDFDLKEIGKAKVVLYVIIPVMDNTYESFINLFFSQLFDELYKLAADHHAKLPH
QVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQALYGKDKAESILGNHAVKICLNAANDV
TAKYFSDLLGKSTVKVETGSESTSHSKEESHSKSDSYSYTSRSLMTPDEIMRMPEDQSLLIFSNARPVKA
TKAFQFKLFPGADHLVNLSQNDYQGQPEASQETNFHQRVEKWKAKLKETAQKKAANKMSQTEEEQQQDNL
DSDLERVSNKDSQTEEPEEDDDNNLFG
MNGTILGIVDKRIVYQNNSTKPNRNIFVVGGPGSYKTQSVVITNLFNETENSIVVTDPKGELYEKTAGIK
LAQGYQVHVVNFANMAHSDRYNPFDYIQRDIQAETVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMN
HRPPEQRNLAGVTQVLQENDVEAEEKGADSPLDTLFLDLTMTDPARRAYELGFKKAKGEMKASIIESLLA
TISKFVDKEVANFTSFSDFDLKEIGKAKVVLYVIIPVMDNTYESFINLFFSQLFDELYKLAADHHAKLPH
QVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQALYGKDKAESILGNHAVKICLNAANDV
TAKYFSDLLGKSTVKVETGSESTSHSKEESHSKSDSYSYTSRSLMTPDEIMRMPEDQSLLIFSNARPVKA
TKAFQFKLFPGADHLVNLSQNDYQGQPEASQETNFHQRVEKWKAKLKETAQKKAANKMSQTEEEQQQDNL
DSDLERVSNKDSQTEEPEEDDDNNLFG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 6116..16020
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NOS86_RS13575 (NOS86_13575) | 1198..1878 | + | 681 | WP_010012556 | zeta toxin family protein | - |
| NOS86_RS13580 (NOS86_13580) | 1868..2146 | - | 279 | WP_064522592 | hypothetical protein | - |
| NOS86_RS13585 (NOS86_13585) | 2169..2396 | - | 228 | WP_003555660 | hypothetical protein | - |
| NOS86_RS13590 (NOS86_13590) | 2650..4713 | + | 2064 | WP_064522591 | MobQ family relaxase | - |
| NOS86_RS13595 (NOS86_13595) | 4797..5105 | + | 309 | WP_082272744 | hypothetical protein | - |
| NOS86_RS13600 (NOS86_13600) | 5144..5758 | + | 615 | WP_064522590 | hypothetical protein | - |
| NOS86_RS13605 (NOS86_13605) | 5760..6095 | + | 336 | WP_003660303 | CagC family type IV secretion system protein | - |
| NOS86_RS13610 (NOS86_13610) | 6116..6478 | + | 363 | WP_064522589 | hypothetical protein | trsC |
| NOS86_RS13615 (NOS86_13615) | 6447..7106 | + | 660 | WP_054769761 | TrsD/TraD family conjugative transfer protein | trsD |
| NOS86_RS13620 (NOS86_13620) | 7118..9133 | + | 2016 | WP_064522588 | DUF87 domain-containing protein | virb4 |
| NOS86_RS13625 (NOS86_13625) | 9126..10544 | + | 1419 | WP_064522587 | type VII secretion protein EssB/YukC | trsF |
| NOS86_RS13630 (NOS86_13630) | 10545..11699 | + | 1155 | WP_064522586 | phage tail tip lysozyme | prgK |
| NOS86_RS13635 (NOS86_13635) | 11713..12330 | + | 618 | WP_064522585 | hypothetical protein | - |
| NOS86_RS13640 (NOS86_13640) | 12317..12685 | + | 369 | WP_064522584 | thioredoxin family protein | - |
| NOS86_RS13645 (NOS86_13645) | 12686..13171 | + | 486 | WP_064522583 | hypothetical protein | trsJ |
| NOS86_RS13650 (NOS86_13650) | 13168..13557 | + | 390 | WP_057738183 | hypothetical protein | - |
| NOS86_RS13655 (NOS86_13655) | 13573..15126 | + | 1554 | WP_064522582 | VirD4-like conjugal transfer protein, CD1115 family | - |
| NOS86_RS13660 (NOS86_13660) | 15151..16020 | + | 870 | WP_064522581 | conjugal transfer protein TrbL family protein | trsL |
| NOS86_RS13665 (NOS86_13665) | 16040..16438 | + | 399 | WP_016373478 | hypothetical protein | - |
| NOS86_RS13670 (NOS86_13670) | 16445..18604 | + | 2160 | WP_108271823 | type IA DNA topoisomerase | - |
| NOS86_RS13675 (NOS86_13675) | 18725..18940 | + | 216 | WP_064522596 | hypothetical protein | - |
| NOS86_RS13680 (NOS86_13680) | 18943..20067 | + | 1125 | WP_064522595 | ArdC-like ssDNA-binding domain-containing protein | - |
| NOS86_RS13685 (NOS86_13685) | 20220..20309 | - | 90 | WP_072672334 | putative holin-like toxin | - |
| NOS86_RS13690 (NOS86_13690) | 20494..20937 | + | 444 | WP_016526921 | hypothetical protein | - |
Host bacterium
| ID | 6360 | GenBank | NZ_CP101644 |
| Plasmid name | CLK 101|unnamed1 | Incompatibility group | - |
| Plasmid size | 25480 bp | Coordinate of oriT [Strand] | 2528..2547 [-] |
| Host baterium | Lacticaseibacillus rhamnosus strain CLK 101 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |