Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105899 |
| Name | oriT_pFJMB80378_1  |
| Organism | Enterobacter hormaechei strain FUJ80378 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_AP025920 (251289..251543 [-], 255 nt) |
| oriT length | 255 nt |
| IRs (inverted repeats) | 153..158, 160..165 (AAAAGT..ACTTTT) 122..130, 135..143 (TTAAGGCTT..AAGCCTTAA) 49..55, 60..66 (GAATTTT..AAAATTC) |
| Location of nic site | 78..79 |
| Conserved sequence flanking the nic site |
TTTGGTTAAA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 255 nt
>oriT_pFJMB80378_1
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3983 | GenBank | WP_251266345 |
| Name | TraI_2_NGK71_RS25790_pFJMB80378_1 |
UniProt ID | _ |
| Length | 181 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 181 a.a. Molecular weight: 20523.58 Da Isoelectric Point: 6.2129
>WP_251266345.1 TraI domain-containing protein [Enterobacter hormaechei]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEVTCSPLISTPRC
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEVTCSPLISTPRC
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 3988 | GenBank | WP_276532224 |
| Name | traD_NGK71_RS25800_pFJMB80378_1 |
UniProt ID | _ |
| Length | 379 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 379 a.a. Molecular weight: 42015.16 Da Isoelectric Point: 6.9624
>WP_276532224.1 conjugative transfer system coupling protein TraD [Enterobacter hormaechei]
MGAGHSIYLIHKNMKSHMSIVNLTVKVMESCYARYYGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMN
YEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIMPVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFS
TGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGSRYNAQDGDMSANSRISIFVDEAHSAINNPMINLLA
QGRAAKIALFICTQTISDFIAAASVETANRITGLCNNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTT
GSETSLPHNNFSGSISERKQTTLEESIPKDLLGQVPMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLS
EMLFKKAGKVTLRQNLDIKNLNKFLRKLH
MGAGHSIYLIHKNMKSHMSIVNLTVKVMESCYARYYGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMN
YEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIMPVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFS
TGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGSRYNAQDGDMSANSRISIFVDEAHSAINNPMINLLA
QGRAAKIALFICTQTISDFIAAASVETANRITGLCNNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTT
GSETSLPHNNFSGSISERKQTTLEESIPKDLLGQVPMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLS
EMLFKKAGKVTLRQNLDIKNLNKFLRKLH
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3661..12868
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NGK71_RS24405 (FJMB80378_47920) | 1..876 | + | 876 | WP_000594611 | RepB family plasmid replication initiator protein | - |
| NGK71_RS24410 | 1901..3048 | + | 1148 | WP_085949497 | IS3 family transposase | - |
| NGK71_RS24415 (FJMB80378_47960) | 3257..3610 | + | 354 | WP_000423602 | hypothetical protein | - |
| NGK71_RS24420 (FJMB80378_47970) | 3628..3978 | + | 351 | WP_001447770 | type IV conjugative transfer system protein TraL | - |
| NGK71_RS24425 (FJMB80378_47980) | 3990..4778 | + | 789 | WP_000783153 | TraE/TraK family type IV conjugative transfer system protein | traE |
| NGK71_RS24430 (FJMB80378_47990) | 4778..6049 | + | 1272 | WP_000592092 | type-F conjugative transfer system secretin TraK | traK |
| NGK71_RS24435 | 6051..6512 | + | 462 | WP_000521242 | plasmid transfer protein HtdO | - |
| NGK71_RS24440 (FJMB80378_48010) | 6502..7857 | + | 1356 | WP_000351841 | IncHI-type conjugal transfer protein TrhB | traB |
| NGK71_RS24445 (FJMB80378_48020) | 7865..8347 | + | 483 | WP_000377632 | hypothetical protein | - |
| NGK71_RS24450 (FJMB80378_48030) | 8466..9218 | + | 753 | WP_182911723 | protein-disulfide isomerase HtdT | - |
| NGK71_RS24455 (FJMB80378_48040) | 9228..10178 | + | 951 | WP_001022588 | IncHI-type conjugal transfer lipoprotein TrhV | traV |
| NGK71_RS24460 (FJMB80378_48050) | 10187..12868 | + | 2682 | WP_000387412 | TraC family protein | virb4 |
| NGK71_RS24465 | 15417..15578 | + | 162 | WP_001371949 | hypothetical protein | - |
Host bacterium
| ID | 6336 | GenBank | NZ_AP025920 |
| Plasmid name | pFJMB80378_1 | Incompatibility group | IncHI2A |
| Plasmid size | 372129 bp | Coordinate of oriT [Strand] | 251289..251543 [-] |
| Host baterium | Enterobacter hormaechei strain FUJ80378 |
Cargo genes
| Drug resistance gene | sul1, qacE, aac(6')-IIc, blaIMP-1, qnrB6, tet(B) |
| Virulence gene | - |
| Metal resistance gene | terW, terZ, terA, terB, terC, terD, terE, merE, merD, merB, merR2, merA, merP, merT, merR1, merR, silE, silR, arsH, arsR, arsB, arsC, rcnR/yohL, ncrC |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |