Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105873 |
| Name | oriT_pFJMB80013_2  |
| Organism | Enterobacter hormaechei strain FUJ80013 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_AP025789 (36596..36700 [+], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_pFJMB80013_2
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3968 | GenBank | WP_085844212 |
| Name | Relaxase_NGJ87_RS25205_pFJMB80013_2 |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75246.00 Da Isoelectric Point: 9.9948
>WP_085844212.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLIRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQNTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSENDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLIRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQNTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSENDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 3979 | GenBank | WP_023302493 |
| Name | t4cp2_NGJ87_RS24935_pFJMB80013_2 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_023302493.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 39498..58697
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NGJ87_RS25185 (FJMB80013_49300) | 34582..34893 | + | 312 | WP_251279850 | hypothetical protein | - |
| NGJ87_RS25190 (FJMB80013_49310) | 35026..35562 | + | 537 | WP_251279851 | hypothetical protein | - |
| NGJ87_RS25195 (FJMB80013_49330) | 36066..36428 | - | 363 | WP_251854797 | MobC | - |
| NGJ87_RS25200 (FJMB80013_49340) | 36704..37021 | + | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| NGJ87_RS25205 (FJMB80013_49350) | 37008..38987 | + | 1980 | WP_085844212 | TraI/MobA(P) family conjugative relaxase | - |
| NGJ87_RS25210 (FJMB80013_49360) | 39001..39501 | + | 501 | WP_251279826 | DotD/TraH family lipoprotein | - |
| NGJ87_RS25215 (FJMB80013_49370) | 39498..40277 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| NGJ87_RS25220 (FJMB80013_49380) | 40288..41451 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| NGJ87_RS25225 (FJMB80013_49390) | 41441..41701 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| NGJ87_RS25230 (FJMB80013_49400) | 41726..45073 | + | 3348 | WP_225577820 | LPD7 domain-containing protein | - |
| NGJ87_RS25235 (FJMB80013_49410) | 45039..45551 | + | 513 | WP_011091071 | hypothetical protein | traL |
| NGJ87_RS25240 (FJMB80013_49420) | 45552..45764 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| NGJ87_RS25245 (FJMB80013_49430) | 45736..46146 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| NGJ87_RS25250 (FJMB80013_49440) | 46214..46927 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| NGJ87_RS25255 | 46936..48087 | + | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| NGJ87_RS25260 (FJMB80013_49470) | 48099..49448 | + | 1350 | WP_015062830 | conjugal transfer protein TraO | traO |
| NGJ87_RS25265 (FJMB80013_49480) | 49460..50164 | + | 705 | WP_040119747 | conjugal transfer protein TraP | traP |
| NGJ87_RS25270 (FJMB80013_49490) | 50188..50718 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| NGJ87_RS25275 (FJMB80013_49500) | 50735..51124 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| NGJ87_RS25280 (FJMB80013_49510) | 51170..51664 | + | 495 | WP_011091080 | hypothetical protein | - |
| NGJ87_RS25285 (FJMB80013_49520) | 51661..54711 | + | 3051 | WP_040119746 | conjugal transfer protein | traU |
| NGJ87_RS25290 (FJMB80013_49530) | 54708..55916 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| NGJ87_RS25295 (FJMB80013_49540) | 55913..56509 | + | 597 | WP_015060003 | hypothetical protein | - |
| NGJ87_RS25300 (FJMB80013_49550) | 56502..58697 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| NGJ87_RS25305 (FJMB80013_49560) | 58699..59352 | + | 654 | WP_015062835 | hypothetical protein | - |
| NGJ87_RS25310 (FJMB80013_49570) | 59434..59664 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 6310 | GenBank | NZ_AP025789 |
| Plasmid name | pFJMB80013_2 | Incompatibility group | IncL/M |
| Plasmid size | 59961 bp | Coordinate of oriT [Strand] | 36596..36700 [+] |
| Host baterium | Enterobacter hormaechei strain FUJ80013 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |