Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 105859
• Name: oriT_I4/6O|p2
• Organism: Lactococcus formosensis strain I4/6O
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP086398 (23297..23433 [+], 137 nt)
• oriT length: 137 nt
• IRs (inverted repeats): 21..28, 42..49 (AAAAGGGG..CCCCTTTT) 25..30, 39..44 (GGGGAA..TTCCCC)
• Location of nic site: 104..105
• Conserved sequence flanking the nic site: GCTTGCAGTA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 137 nt

>oriT_I4/6O|p2
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATAGTCTATTTTGTT

Relaxase

Relaxase_LMK00_RS12020_I4/6O|p2

• ID: 3957
• GenBank: WP_252175982
• Name: Relaxase_LMK00_RS12020_I4/6O|p2
• UniProt ID: _
• Length: 410 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 410 a.a.
• Molecular weight: 47721.43 Da
• Isoelectric Point: 9.0605

>WP_252175982.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactococcus]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFRAVEQREPAITPLERDTQQRESEIVILEQAIEPRKSEALKRESTINRFIDTLKQLAGRVPQLTQ
RVTRKLKQTKDKILDDFERRFSKDMKNYEKEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-280)

Protein structure

No available structure.

Host bacterium

• ID: 6296
• GenBank: NZ_CP086398
• Plasmid name: I4/6O|p2
• Incompatibility group: -
• Plasmid size: 26734 bp
• Coordinate of oriT [Strand]: 23297..23433 [+]
• Host baterium: Lactococcus formosensis strain I4/6O

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -