Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105836 |
| Name | oriT_pECL189-4  |
| Organism | Enterobacter hormaechei strain 189 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP047969 (12212..12294 [-], 83 nt) |
| oriT length | 83 nt |
| IRs (inverted repeats) | 27..34, 37..44 (AGTGCGAT..ATCGCACT) 13..18, 32..37 (TAAATC..GATTTA) |
| Location of nic site | 55..56 |
| Conserved sequence flanking the nic site |
GGTGTATAGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 83 nt
>oriT_pECL189-4
TTTTTTGTTTTTTAAATCAGTTAGTTAGTGCGATTTATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC
TTTTTTGTTTTTTAAATCAGTTAGTTAGTGCGATTTATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3940 | GenBank | WP_014014966 |
| Name | mobF_GUY47_RS26305_pECL189-4 |
UniProt ID | I7CZU5 |
| Length | 1068 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1068 a.a. Molecular weight: 118452.14 Da Isoelectric Point: 6.4309
>WP_014014966.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNDQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGTTEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKKRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSDGQQEKSYGHDAGHQTEEKENTMKNATEAAHDGPDTSGT
ENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARDVELP
QHEHENHQHDHDKEGYGL
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNDQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGTTEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKKRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSDGQQEKSYGHDAGHQTEEKENTMKNATEAAHDGPDTSGT
ENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARDVELP
QHEHENHQHDHDKEGYGL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | I7CZU5 |
T4CP
| ID | 3956 | GenBank | WP_014014965 |
| Name | t4cp2_GUY47_RS26300_pECL189-4 |
UniProt ID | _ |
| Length | 510 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 510 a.a. Molecular weight: 58174.11 Da Isoelectric Point: 9.7081
>WP_014014965.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVAIIAGAVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAERTKERGHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGRDKRVILDPNGEYVETFYRENDIILNPYDKRSPGWSMFNEIRDEFDYDLYAVSIIQ
ESKERSAEEWYRYGRLLYREVSKKLLTHKRNPSMEEVLYWCNVAEYDKVKDFVKGTPAESLFIKGAERAT
GSARFVLGDKLASHLKMPEGNFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTFSQLVERYGINIAETLLGSMRSGIVMGSSRL
GKATLEHMSRALGEIEGEIERRQGGILQSGPNNRPRIETRTVRAVTPTEISELRNLTGYIYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVAIIAGAVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAERTKERGHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGRDKRVILDPNGEYVETFYRENDIILNPYDKRSPGWSMFNEIRDEFDYDLYAVSIIQ
ESKERSAEEWYRYGRLLYREVSKKLLTHKRNPSMEEVLYWCNVAEYDKVKDFVKGTPAESLFIKGAERAT
GSARFVLGDKLASHLKMPEGNFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTFSQLVERYGINIAETLLGSMRSGIVMGSSRL
GKATLEHMSRALGEIEGEIERRQGGILQSGPNNRPRIETRTVRAVTPTEISELRNLTGYIYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 28758..39040
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GUY47_RS26345 (GUY47_26350) | 24111..24923 | - | 813 | WP_004201164 | subclass B1 metallo-beta-lactamase NDM-1 | - |
| GUY47_RS26350 (GUY47_26360) | 25219..26250 | + | 1032 | WP_001395480 | IS630-like element ISEc33 family transposase | - |
| GUY47_RS26355 (GUY47_26365) | 26269..27015 | - | 747 | Protein_32 | IS30-like element ISAba125 family transposase | - |
| GUY47_RS26360 (GUY47_26370) | 28234..28746 | - | 513 | WP_029697418 | phospholipase D family protein | - |
| GUY47_RS26365 (GUY47_26375) | 28758..29759 | - | 1002 | WP_001076634 | ATPase, T2SS/T4P/T4SS family | virB11 |
| GUY47_RS26370 (GUY47_26380) | 29810..30949 | - | 1140 | WP_014014945 | type IV secretion system protein VirB10 | virB10 |
| GUY47_RS26375 (GUY47_26385) | 30949..31836 | - | 888 | WP_014014946 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| GUY47_RS26380 (GUY47_26390) | 31847..32542 | - | 696 | WP_014014947 | type IV secretion system protein | virB8 |
| GUY47_RS26790 | 32553..32684 | - | 132 | WP_072000589 | conjugal transfer protein TraN | - |
| GUY47_RS26385 (GUY47_26395) | 32761..33798 | - | 1038 | WP_014014948 | type IV secretion system protein | virB6 |
| GUY47_RS26390 (GUY47_26400) | 33815..34057 | - | 243 | WP_000858958 | EexN family lipoprotein | - |
| GUY47_RS26395 (GUY47_26405) | 34067..34768 | - | 702 | WP_014014949 | type IV secretion system protein | virB5 |
| GUY47_RS26400 (GUY47_26410) | 34784..37357 | - | 2574 | WP_014014950 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| GUY47_RS26405 (GUY47_26415) | 37358..37675 | - | 318 | WP_000462629 | VirB3 family type IV secretion system protein | virB3 |
| GUY47_RS26410 (GUY47_26420) | 37715..38011 | - | 297 | WP_015060075 | TrbC/VirB2 family protein | virB2 |
| GUY47_RS26415 (GUY47_26425) | 38258..39040 | - | 783 | WP_014014952 | lytic transglycosylase domain-containing protein | virB1 |
| GUY47_RS26420 (GUY47_26430) | 39140..39457 | + | 318 | WP_000960955 | H-NS family nucleoid-associated regulatory protein | - |
| GUY47_RS26425 (GUY47_26435) | 39461..39811 | + | 351 | WP_014014953 | hypothetical protein | - |
| GUY47_RS26430 (GUY47_26440) | 39808..40146 | + | 339 | WP_000040982 | hypothetical protein | - |
| GUY47_RS26435 (GUY47_26445) | 40150..40458 | + | 309 | WP_000733764 | hypothetical protein | - |
| GUY47_RS26440 (GUY47_26450) | 40743..40928 | + | 186 | WP_014014955 | ribbon-helix-helix domain-containing protein | - |
Host bacterium
| ID | 6273 | GenBank | NZ_CP047969 |
| Plasmid name | pECL189-4 | Incompatibility group | IncN2 |
| Plasmid size | 41439 bp | Coordinate of oriT [Strand] | 12212..12294 [-] |
| Host baterium | Enterobacter hormaechei strain 189 |
Cargo genes
| Drug resistance gene | blaNDM-1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |