Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105835 |
| Name | oriT_pECL189-3  |
| Organism | Enterobacter hormaechei strain 189 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP047968 (71530..71616 [-], 87 nt) |
| oriT length | 87 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 87 nt
>oriT_pECL189-3
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3939 | GenBank | WP_022650008 |
| Name | Relaxase_GUY47_RS26050_pECL189-3 |
UniProt ID | A0A4Q2QTK9 |
| Length | 899 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 102314.37 Da Isoelectric Point: 8.2756
>WP_022650008.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Q2QTK9 |
T4CP
| ID | 3955 | GenBank | WP_022650006 |
| Name | trbC_GUY47_RS26065_pECL189-3 |
UniProt ID | _ |
| Length | 766 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 766 a.a. Molecular weight: 87660.94 Da Isoelectric Point: 6.8660
>WP_022650006.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 77739..103968
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GUY47_RS26055 (GUY47_26060) | 74755..74958 | - | 204 | WP_123839396 | hypothetical protein | - |
| GUY47_RS26060 (GUY47_26065) | 75092..75361 | - | 270 | WP_022650007 | hypothetical protein | - |
| GUY47_RS26065 (GUY47_26070) | 75435..77735 | - | 2301 | WP_022650006 | F-type conjugative transfer protein TrbC | - |
| GUY47_RS26070 (GUY47_26075) | 77739..78995 | - | 1257 | WP_126530067 | hypothetical protein | trbB |
| GUY47_RS26075 (GUY47_26080) | 78976..80184 | - | 1209 | WP_022650004 | hypothetical protein | trbA |
| GUY47_RS26080 (GUY47_26085) | 80505..81137 | - | 633 | WP_022650003 | plasmid IncI1-type surface exclusion protein ExcA | - |
| GUY47_RS26085 (GUY47_26090) | 81199..83358 | - | 2160 | WP_022650002 | DotA/TraY family protein | traY |
| GUY47_RS26090 (GUY47_26095) | 83416..83958 | - | 543 | WP_022650001 | conjugal transfer protein TraX | - |
| GUY47_RS26095 (GUY47_26100) | 83955..85157 | - | 1203 | WP_022650000 | conjugal transfer protein TraW | traW |
| GUY47_RS26100 (GUY47_26105) | 85170..85739 | - | 570 | WP_022649999 | hypothetical protein | traV |
| GUY47_RS26105 (GUY47_26110) | 85739..88792 | - | 3054 | WP_022649998 | hypothetical protein | traU |
| GUY47_RS26110 (GUY47_26115) | 88879..89604 | - | 726 | WP_063864836 | hypothetical protein | traT |
| GUY47_RS26115 (GUY47_26120) | 89788..90192 | - | 405 | WP_022649996 | DUF6750 family protein | traR |
| GUY47_RS26120 (GUY47_26125) | 90227..90751 | - | 525 | WP_022649995 | conjugal transfer protein TraQ | traQ |
| GUY47_RS26125 (GUY47_26130) | 90751..91524 | - | 774 | WP_123839398 | conjugal transfer protein TraP | traP |
| GUY47_RS26130 (GUY47_26135) | 91505..92812 | - | 1308 | WP_022649993 | conjugal transfer protein TraO | traO |
| GUY47_RS26135 (GUY47_26140) | 92812..93792 | - | 981 | WP_022649992 | DotH/IcmK family type IV secretion protein | traN |
| GUY47_RS26140 (GUY47_26145) | 93803..94420 | - | 618 | WP_179297403 | DotI/IcmL family type IV secretion protein | traM |
| GUY47_RS26145 (GUY47_26150) | 94510..94860 | - | 351 | WP_022649990 | hypothetical protein | traL |
| GUY47_RS26150 (GUY47_26155) | 94878..98420 | - | 3543 | WP_032635082 | LPD7 domain-containing protein | - |
| GUY47_RS26155 (GUY47_26160) | 98492..98779 | - | 288 | WP_022649988 | hypothetical protein | traK |
| GUY47_RS26160 (GUY47_26165) | 98776..99936 | - | 1161 | WP_022649987 | plasmid transfer ATPase TraJ | virB11 |
| GUY47_RS26165 (GUY47_26170) | 99949..100755 | - | 807 | WP_022649986 | type IV secretory system conjugative DNA transfer family protein | traI |
| GUY47_RS26170 (GUY47_26175) | 100752..101225 | - | 474 | WP_022649985 | DotD/TraH family lipoprotein | - |
| GUY47_RS26175 (GUY47_26180) | 101296..101712 | - | 417 | WP_123839394 | hypothetical protein | - |
| GUY47_RS26180 (GUY47_26185) | 101821..103026 | - | 1206 | WP_022649984 | conjugal transfer protein TraF | - |
| GUY47_RS26185 (GUY47_26190) | 103132..103968 | - | 837 | WP_022649983 | hypothetical protein | traE |
| GUY47_RS26190 (GUY47_26195) | 104115..104591 | - | 477 | WP_022649982 | hypothetical protein | - |
| GUY47_RS26195 (GUY47_26200) | 104611..105771 | - | 1161 | WP_022649981 | site-specific integrase | - |
Host bacterium
| ID | 6272 | GenBank | NZ_CP047968 |
| Plasmid name | pECL189-3 | Incompatibility group | - |
| Plasmid size | 106698 bp | Coordinate of oriT [Strand] | 71530..71616 [-] |
| Host baterium | Enterobacter hormaechei strain 189 |
Cargo genes
| Drug resistance gene | blaLAP-2, qnrS1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIC1, AcrIIA7 |