Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105834
Name   oriT_pECL189-1 in_silico
Organism   Enterobacter hormaechei strain 189
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP047966 (85830..85924 [+], 95 nt)
oriT length   95 nt
IRs (inverted repeats)      73..78, 85..90  (AAAAAA..TTTTTT)
 73..78, 84..89  (AAAAAA..TTTTTT)
 27..34, 37..44  (AGCGTGAT..ATCACGCT)
 13..19, 31..37  (TAAATCA..TGATTTA)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 95 nt

>oriT_pECL189-1
TTTTTTTTCTTTTAAATCAGTGAGATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   3954 GenBank   WP_044704830
Name   t4cp2_GUY47_RS24945_pECL189-1 insolico UniProt ID   _
Length   653 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 653 a.a.        Molecular weight: 72734.19 Da        Isoelectric Point: 6.8597

>WP_044704830.1 MULTISPECIES: TraM recognition domain-containing protein [Enterobacterales]
MLNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFFGLAELWAGRMRFVGLSHKERREYAD
RYKRDNDRAAPFSVLASLDSPGRMVRARRHVWNYAYWLTGAAGLVLLGGLVVAVFIESWGETVLRPGYYA
NADYYLLQPANLFGIAWCAGGLVVGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVR
HLHFGEPVPVNALADFSTEQARQQQVVFLGKDEDGQPVRVPREAWRKTNIQILGLPGSGKSVMATNALIR
CVRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLVAGFNLAETGD
VADHYRISEQKAAKLIAEQFPHGANIQQVLAAAYALPEALKKDVKGLITKLENVADLSVLQTDSGIDVAG
IINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARDEFRAWPHVSVMLDEIKYLLSKYVLNALGTLRS
RDCNLLLAHQSLGDFGQCGQDLPADFVKTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASR
EAGNVEHISGDSFIQKEARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTD
PLAEYQAEAVRQRPGDDDFAGLY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(465-591)

  Protein structure



No available structure.




Host bacterium

ID   6271 GenBank   NZ_CP047966
Plasmid name   pECL189-1 Incompatibility group   IncP6
Plasmid size   133022 bp Coordinate of oriT [Strand]   85830..85924 [+]
Host baterium   Enterobacter hormaechei strain 189

Cargo genes

Drug resistance gene   aac(6')-Ib, ant(3'')-Ia, blaOXA-9, blaTEM-1A, sul2, aph(3'')-Ib, aph(6)-Id, catA2, tet(D), dfrA14, blaSHV-12, blaKPC-2
Virulence gene   -
Metal resistance gene   merE, merD, merA, merR, merT, merP, merC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -