Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105794
Name   oriT_BW|unnamed3 in_silico
Organism   Enterobacter hormaechei strain BW
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP023571 (45165..45251 [-], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_BW|unnamed3
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3915 GenBank   WP_022650008
Name   Relaxase_CPT31_RS24695_BW|unnamed3 insolico UniProt ID   A0A4Q2QTK9
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102314.37 Da        Isoelectric Point: 8.2756

>WP_022650008.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(51-317)


  Protein structure


Source ID Structure
AlphaFold DB A0A4Q2QTK9


T4CP

ID   3928 GenBank   WP_022650006
Name   trbC_CPT31_RS24710_BW|unnamed3 insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87660.94 Da        Isoelectric Point: 6.8660

>WP_022650006.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 51374..85818

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPT31_RS24700 48390..48593 - 204 WP_123839396 hypothetical protein -
CPT31_RS24705 (CPT31_25330) 48727..48996 - 270 WP_022650007 hypothetical protein -
CPT31_RS24710 (CPT31_25335) 49070..51370 - 2301 WP_022650006 F-type conjugative transfer protein TrbC -
CPT31_RS24715 (CPT31_25340) 51374..52630 - 1257 WP_126530067 hypothetical protein trbB
CPT31_RS24720 (CPT31_25345) 52611..53819 - 1209 WP_022650004 hypothetical protein trbA
CPT31_RS24725 (CPT31_25350) 54140..54772 - 633 WP_022650003 plasmid IncI1-type surface exclusion protein ExcA -
CPT31_RS24730 (CPT31_25355) 54834..56993 - 2160 WP_022650002 DotA/TraY family protein traY
CPT31_RS24735 (CPT31_25360) 57051..57593 - 543 WP_022650001 conjugal transfer protein TraX -
CPT31_RS24740 (CPT31_25365) 57590..58792 - 1203 WP_022650000 conjugal transfer protein TraW traW
CPT31_RS24745 (CPT31_25370) 58805..59374 - 570 WP_022649999 hypothetical protein traV
CPT31_RS24750 (CPT31_25375) 59374..62427 - 3054 WP_022649998 hypothetical protein traU
CPT31_RS24755 (CPT31_25380) 62514..63239 - 726 WP_063864836 hypothetical protein traT
CPT31_RS24760 (CPT31_25385) 63423..63827 - 405 WP_022649996 DUF6750 family protein traR
CPT31_RS24765 (CPT31_25390) 63862..64386 - 525 WP_022649995 conjugal transfer protein TraQ traQ
CPT31_RS24770 (CPT31_25395) 64386..65159 - 774 WP_123839398 conjugal transfer protein TraP traP
CPT31_RS24775 (CPT31_25400) 65140..66447 - 1308 WP_022649993 conjugal transfer protein TraO traO
CPT31_RS24780 (CPT31_25405) 66447..67427 - 981 WP_022649992 DotH/IcmK family type IV secretion protein traN
CPT31_RS24785 (CPT31_25410) 67438..68055 - 618 WP_179297403 DotI/IcmL family type IV secretion protein traM
CPT31_RS24790 (CPT31_25415) 68145..68495 - 351 WP_022649990 hypothetical protein traL
CPT31_RS24795 (CPT31_25420) 68513..72055 - 3543 WP_032635082 LPD7 domain-containing protein -
CPT31_RS24800 (CPT31_25425) 72127..72414 - 288 WP_022649988 hypothetical protein traK
CPT31_RS24805 (CPT31_25430) 72411..73571 - 1161 WP_022649987 plasmid transfer ATPase TraJ virB11
CPT31_RS24810 (CPT31_25435) 73584..74390 - 807 WP_022649986 type IV secretory system conjugative DNA transfer family protein traI
CPT31_RS24815 (CPT31_25440) 74387..74860 - 474 WP_022649985 DotD/TraH family lipoprotein -
CPT31_RS24820 74931..75347 - 417 WP_123839394 hypothetical protein -
CPT31_RS24825 (CPT31_25445) 75456..76661 - 1206 WP_022649984 conjugal transfer protein TraF -
CPT31_RS24830 (CPT31_25450) 76767..77603 - 837 WP_022649983 hypothetical protein traE
CPT31_RS24835 77750..78226 - 477 WP_022649982 hypothetical protein -
CPT31_RS24840 (CPT31_25455) 78246..79406 - 1161 WP_022649981 site-specific integrase -
CPT31_RS24845 (CPT31_25460) 79984..81321 - 1338 WP_022649980 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
CPT31_RS24850 (CPT31_25465) 81318..81992 - 675 WP_022649979 prepilin peptidase -
CPT31_RS24855 (CPT31_25470) 81977..82459 - 483 WP_045261878 lytic transglycosylase domain-containing protein virB1
CPT31_RS24860 (CPT31_25475) 82544..83158 - 615 WP_022649977 type 4 pilus major pilin -
CPT31_RS24865 (CPT31_25480) 83172..84266 - 1095 WP_032623575 type II secretion system F family protein -
CPT31_RS24870 (CPT31_25485) 84259..85818 - 1560 WP_022649975 ATPase, T2SS/T4P/T4SS family virB11
CPT31_RS24875 (CPT31_25490) 85821..86336 - 516 WP_022649974 type IV pilus biogenesis protein PilP -
CPT31_RS24880 (CPT31_25495) 86320..87624 - 1305 WP_022649973 type 4b pilus protein PilO2 -
CPT31_RS24885 (CPT31_25500) 87626..89302 - 1677 WP_022649972 PilN family type IVB pilus formation outer membrane protein -
CPT31_RS24890 (CPT31_25505) 89317..89754 - 438 WP_022649971 type IV pilus biogenesis protein PilM -

Host bacterium

ID   6231 GenBank   NZ_CP023571
Plasmid name   BW|unnamed3 Incompatibility group   -
Plasmid size   118105 bp Coordinate of oriT [Strand]   45165..45251 [-]
Host baterium   Enterobacter hormaechei strain BW

Cargo genes

Drug resistance gene   dfrA12, aadA2, qacE, sul1, blaLAP-2, qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIC1, AcrIIA7