Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105789 |
| Name | oriT_pC15_002  |
| Organism | Enterobacter hormaechei strain C15 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP042490 (25113..25218 [-], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pC15_002
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3913 | GenBank | WP_050868819 |
| Name | Relaxase_FR808_RS25080_pC15_002 |
UniProt ID | A0A142BMF1 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75234.98 Da Isoelectric Point: 10.0285
>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142BMF1 |
T4CP
| ID | 3925 | GenBank | WP_011091092 |
| Name | t4cp2_FR808_RS25350_pC15_002 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79491.81 Da Isoelectric Point: 4.8270
>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3169..22317
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FR808_RS24970 (FR808_24970) | 855..1910 | - | 1056 | WP_011091086 | plasmid replication initiator RepA | - |
| FR808_RS24975 (FR808_24975) | 2208..2438 | - | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
| FR808_RS24980 (FR808_24980) | 2513..3166 | - | 654 | WP_011091084 | hypothetical protein | - |
| FR808_RS24985 (FR808_24985) | 3169..5349 | - | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| FR808_RS24990 (FR808_24990) | 5342..5992 | - | 651 | WP_011091083 | hypothetical protein | - |
| FR808_RS24995 (FR808_24995) | 5989..7197 | - | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| FR808_RS25000 (FR808_25000) | 7194..10244 | - | 3051 | WP_011091081 | hypothetical protein | traU |
| FR808_RS25005 (FR808_25005) | 10241..10735 | - | 495 | WP_011091080 | hypothetical protein | - |
| FR808_RS25010 (FR808_25010) | 10781..11170 | - | 390 | WP_011091078 | DUF6750 family protein | traR |
| FR808_RS25015 (FR808_25015) | 11187..11717 | - | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| FR808_RS25020 (FR808_25020) | 11741..12445 | - | 705 | WP_011091076 | conjugal transfer protein TraP | traP |
| FR808_RS25025 (FR808_25025) | 12457..13806 | - | 1350 | WP_011091075 | conjugal transfer protein TraO | traO |
| FR808_RS25030 (FR808_25030) | 13818..14969 | - | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| FR808_RS25035 (FR808_25035) | 14978..15691 | - | 714 | WP_109914255 | DotI/IcmL family type IV secretion protein | traM |
| FR808_RS25040 (FR808_25040) | 15759..16169 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| FR808_RS25045 (FR808_25045) | 16198..16353 | + | 156 | WP_172693602 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| FR808_RS25050 (FR808_25050) | 16354..16866 | - | 513 | WP_011091071 | hypothetical protein | traL |
| FR808_RS25845 | 16832..20089 | - | 3258 | WP_015063358 | LPD7 domain-containing protein | - |
| FR808_RS25060 (FR808_25060) | 20114..20374 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| FR808_RS25065 (FR808_25065) | 20364..21527 | - | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| FR808_RS25070 (FR808_25070) | 21538..22317 | - | 780 | WP_015063359 | type IV secretory system conjugative DNA transfer family protein | traI |
| FR808_RS25075 (FR808_25075) | 22314..22814 | - | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| FR808_RS25080 (FR808_25080) | 22828..24807 | - | 1980 | WP_050868819 | TraI/MobA(P) family conjugative relaxase | - |
| FR808_RS25850 | 24794..25321 | - | 528 | WP_225986328 | plasmid mobilization protein MobA | - |
| FR808_RS25090 (FR808_25090) | 25387..25752 | + | 366 | WP_011091067 | hypothetical protein | - |
| FR808_RS25095 (FR808_25095) | 26253..26789 | - | 537 | WP_011091066 | hypothetical protein | - |
| FR808_RS25100 (FR808_25100) | 26922..27233 | - | 312 | WP_011091065 | hypothetical protein | - |
Host bacterium
| ID | 6226 | GenBank | NZ_CP042490 |
| Plasmid name | pC15_002 | Incompatibility group | IncL/M |
| Plasmid size | 60173 bp | Coordinate of oriT [Strand] | 25113..25218 [-] |
| Host baterium | Enterobacter hormaechei strain C15 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |