Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105766 |
| Name | oriT_DSM 16365|unnamed1  |
| Organism | Lactiplantibacillus argentoratensis strain DSM 16365 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP032752 (52271..52291 [+], 21 nt) |
| oriT length | 21 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 21 nt
>oriT_DSM 16365|unnamed1
AGTGGGGTGTAAGTGCGCATT
AGTGGGGTGTAAGTGCGCATT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3903 | GenBank | WP_121054816 |
| Name | mobQ_LPA65_RS15655_DSM 16365|unnamed1 |
UniProt ID | _ |
| Length | 687 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 687 a.a. Molecular weight: 80384.19 Da Isoelectric Point: 9.6086
>WP_121054816.1 MobQ family relaxase [Lactiplantibacillus argentoratensis]
MAIFHMSFSNISAGKGRSAIASAAYRSGEKLFDDKEGRHYFYVRSIMPESFILTPKNAPAWASDREQLWN
EVEKKDRKSNSRYAKEFNVALPVELSESEQKELLTKYVQENFVDEGMVADVAIHRDHPDNPHAHVMLTNR
PFNPDGTWGIKSKKQYILDKNGNKTYTGTSKYPKSRKILMVDWDKKEKITEWRHNWANSVNQVLAQKNIP
DRISEKSFVEQGIADTPMQHEGINSKRYERKAFNQQVKTYRKSKASYKNMQEKVVNRGHLDSLSKHFSFN
EKKVVKELSHELKTYISLESLDDKRRMLFNWKNSTLIKHAVGEDVTKQLLTINQQESSLKKADELLNKVV
DRTTKKLYPELDFEQTTAAERRELIKETNSEQTIFKGSELNERLMNIRDDLLNQQLLTFTKRPYVGWKLL
MQQEKEVKIELKYTLMIHDDSLESLEHVDQGLLEKYSPIEQQKITRAVKDLRTIMAVKQVIQTQYQEVLR
RAFPNGNFNELPMIKQEQAYTAVMYYDPVLKPCQAETIEQWQANPPQVFSPPEHQQGLAYLSGQLSLDQL
ENHHLQRVLKHDGTKQLFFGECKADPTIKNSQIEKIQKQLKGQQAKDDQYRKANMGHYQPLNYKPVSPNY
YLKTAFSDAIMTVLYARDEDYQRQRQAQGLKETEWEMAKKQRQHQTRNRHEDGGMHL
MAIFHMSFSNISAGKGRSAIASAAYRSGEKLFDDKEGRHYFYVRSIMPESFILTPKNAPAWASDREQLWN
EVEKKDRKSNSRYAKEFNVALPVELSESEQKELLTKYVQENFVDEGMVADVAIHRDHPDNPHAHVMLTNR
PFNPDGTWGIKSKKQYILDKNGNKTYTGTSKYPKSRKILMVDWDKKEKITEWRHNWANSVNQVLAQKNIP
DRISEKSFVEQGIADTPMQHEGINSKRYERKAFNQQVKTYRKSKASYKNMQEKVVNRGHLDSLSKHFSFN
EKKVVKELSHELKTYISLESLDDKRRMLFNWKNSTLIKHAVGEDVTKQLLTINQQESSLKKADELLNKVV
DRTTKKLYPELDFEQTTAAERRELIKETNSEQTIFKGSELNERLMNIRDDLLNQQLLTFTKRPYVGWKLL
MQQEKEVKIELKYTLMIHDDSLESLEHVDQGLLEKYSPIEQQKITRAVKDLRTIMAVKQVIQTQYQEVLR
RAFPNGNFNELPMIKQEQAYTAVMYYDPVLKPCQAETIEQWQANPPQVFSPPEHQQGLAYLSGQLSLDQL
ENHHLQRVLKHDGTKQLFFGECKADPTIKNSQIEKIQKQLKGQQAKDDQYRKANMGHYQPLNYKPVSPNY
YLKTAFSDAIMTVLYARDEDYQRQRQAQGLKETEWEMAKKQRQHQTRNRHEDGGMHL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 3915 | GenBank | WP_057717274 |
| Name | t4cp2_LPA65_RS15595_DSM 16365|unnamed1 |
UniProt ID | _ |
| Length | 514 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 514 a.a. Molecular weight: 57797.02 Da Isoelectric Point: 5.1157
>WP_057717274.1 VirD4-like conjugal transfer protein, CD1115 family [Lactiplantibacillus argentoratensis]
MNGTILGIVDKRIVYQNNSTKPNRNIFVVGGPGSYKTQSVVITNLFNETENSIVVTDPKGELYEKTAGIK
LAQGYQVHVVNFANMAHSDRYNPFDYIQRDIQAETVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMN
HRSPEQRNLAGVTQVLQENDVEAEEKGTDSPLDTLFLELTMTDPARRAYELGFKKAKGEMKASIIESLLA
TISKFVDKEVADFTSFSDFDLKEIGNAKVVLYVIIPVMDNTYESFINLFFSQLFDELYKLAADHHAKLPH
QVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQALYGKDKAESILGNHAVKICLNAANDV
TAKYFSDLLGKSTVKVETGSESTSHSKEESHSKSDSYSYTSRSLMTPDEIMRMPEDQSLLIFSNARPVKA
TKAFQFKLFPGADHLVNLSQNEYQGQPEASQETNFHQRVAKWKAKLKETAQKKAANKMSQTEEEQQQDSL
DSDLERVSNKDSQTEEPDDDNLLG
MNGTILGIVDKRIVYQNNSTKPNRNIFVVGGPGSYKTQSVVITNLFNETENSIVVTDPKGELYEKTAGIK
LAQGYQVHVVNFANMAHSDRYNPFDYIQRDIQAETVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMN
HRSPEQRNLAGVTQVLQENDVEAEEKGTDSPLDTLFLELTMTDPARRAYELGFKKAKGEMKASIIESLLA
TISKFVDKEVADFTSFSDFDLKEIGNAKVVLYVIIPVMDNTYESFINLFFSQLFDELYKLAADHHAKLPH
QVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQALYGKDKAESILGNHAVKICLNAANDV
TAKYFSDLLGKSTVKVETGSESTSHSKEESHSKSDSYSYTSRSLMTPDEIMRMPEDQSLLIFSNARPVKA
TKAFQFKLFPGADHLVNLSQNEYQGQPEASQETNFHQRVAKWKAKLKETAQKKAANKMSQTEEEQQQDSL
DSDLERVSNKDSQTEEPDDDNLLG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 38851..48700
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LPA65_RS15565 (LPA65_15565) | 34820..35944 | - | 1125 | WP_057717426 | hypothetical protein | - |
| LPA65_RS15570 (LPA65_15570) | 35948..36142 | - | 195 | WP_057717427 | hypothetical protein | - |
| LPA65_RS15575 (LPA65_15575) | 36284..38419 | - | 2136 | WP_121054815 | type IA DNA topoisomerase | - |
| LPA65_RS15580 (LPA65_15580) | 38426..38836 | - | 411 | WP_057717276 | hypothetical protein | - |
| LPA65_RS15585 (LPA65_15585) | 38851..39690 | - | 840 | WP_057717275 | conjugal transfer protein TrbL family protein | trsL |
| LPA65_RS15590 (LPA65_15590) | 39709..40098 | - | 390 | WP_056988740 | hypothetical protein | - |
| LPA65_RS15595 (LPA65_15595) | 40113..41657 | - | 1545 | WP_057717274 | VirD4-like conjugal transfer protein, CD1115 family | - |
| LPA65_RS15600 (LPA65_15600) | 41659..42129 | - | 471 | WP_057717273 | hypothetical protein | trsJ |
| LPA65_RS15605 (LPA65_15605) | 42130..42498 | - | 369 | WP_040537815 | thioredoxin family protein | - |
| LPA65_RS15610 (LPA65_15610) | 42485..43102 | - | 618 | WP_057717272 | hypothetical protein | - |
| LPA65_RS15615 (LPA65_15615) | 43117..44271 | - | 1155 | WP_057717271 | phage tail tip lysozyme | prgK |
| LPA65_RS15620 (LPA65_15620) | 44272..45690 | - | 1419 | WP_057717270 | type VII secretion protein EssB/YukC | trsF |
| LPA65_RS15625 (LPA65_15625) | 45683..47698 | - | 2016 | WP_057717269 | DUF87 domain-containing protein | virb4 |
| LPA65_RS15630 (LPA65_15630) | 47710..48369 | - | 660 | WP_056980348 | TrsD/TraD family conjugative transfer protein | trsD |
| LPA65_RS15635 (LPA65_15635) | 48338..48700 | - | 363 | WP_003590770 | conjugal transfer protein | trsC |
| LPA65_RS15640 (LPA65_15640) | 48721..49059 | - | 339 | WP_057717268 | CagC family type IV secretion system protein | - |
| LPA65_RS15645 (LPA65_15645) | 49061..49675 | - | 615 | WP_057717267 | hypothetical protein | - |
| LPA65_RS15650 (LPA65_15650) | 49711..50022 | - | 312 | WP_057717266 | hypothetical protein | - |
| LPA65_RS15655 (LPA65_15655) | 50106..52169 | - | 2064 | WP_121054816 | MobQ family relaxase | - |
| LPA65_RS15660 (LPA65_15660) | 52423..52650 | + | 228 | WP_162985473 | hypothetical protein | - |
| LPA65_RS15665 (LPA65_15665) | 52673..52951 | + | 279 | WP_003646133 | hypothetical protein | - |
| LPA65_RS16815 | 52941..53422 | - | 482 | Protein_54 | hypothetical protein | - |
| LPA65_RS15675 (LPA65_15675) | 53412..53693 | - | 282 | WP_003646131 | type II toxin-antitoxin system RelB/DinJ family antitoxin | - |
Host bacterium
| ID | 6203 | GenBank | NZ_CP032752 |
| Plasmid name | DSM 16365|unnamed1 | Incompatibility group | - |
| Plasmid size | 56374 bp | Coordinate of oriT [Strand] | 52271..52291 [+] |
| Host baterium | Lactiplantibacillus argentoratensis strain DSM 16365 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |