Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105761
Name   oriT_pQnrS1-1502262 in_silico
Organism   Enterobacter hormaechei strain N1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP031572 (18385..18471 [-], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_pQnrS1-1502262
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3901 GenBank   WP_022650008
Name   Relaxase_AXA51_RS23800_pQnrS1-1502262 insolico UniProt ID   A0A4Q2QTK9
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102314.37 Da        Isoelectric Point: 8.2756

>WP_022650008.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(51-317)


  Protein structure


Source ID Structure
AlphaFold DB A0A4Q2QTK9


T4CP

ID   3913 GenBank   WP_022650006
Name   trbC_AXA51_RS23815_pQnrS1-1502262 insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87660.94 Da        Isoelectric Point: 6.8660

>WP_022650006.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 24594..59036

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXA51_RS23805 21610..21813 - 204 WP_123839396 hypothetical protein -
AXA51_RS23810 (AXA51_24095) 21947..22216 - 270 WP_022650007 hypothetical protein -
AXA51_RS23815 (AXA51_24100) 22290..24590 - 2301 WP_022650006 F-type conjugative transfer protein TrbC -
AXA51_RS23820 (AXA51_24105) 24594..25850 - 1257 WP_126530067 hypothetical protein trbB
AXA51_RS23825 (AXA51_24110) 25831..27039 - 1209 WP_022650004 hypothetical protein trbA
AXA51_RS23830 (AXA51_24115) 27360..27992 - 633 WP_022650003 plasmid IncI1-type surface exclusion protein ExcA -
AXA51_RS23835 (AXA51_24120) 28054..30213 - 2160 WP_022650002 DotA/TraY family protein traY
AXA51_RS23840 (AXA51_24125) 30271..30813 - 543 WP_022650001 conjugal transfer protein TraX -
AXA51_RS23845 (AXA51_24130) 30810..32012 - 1203 WP_022650000 conjugal transfer protein TraW traW
AXA51_RS25380 32025..32597 - 573 WP_154590113 conjugal transfer protein TraV traV
AXA51_RS23850 (AXA51_24135) 32594..35647 - 3054 WP_022649998 hypothetical protein traU
AXA51_RS23855 (AXA51_24140) 35734..36459 - 726 WP_063864836 hypothetical protein traT
AXA51_RS23860 (AXA51_24145) 36642..37046 - 405 WP_022649996 DUF6750 family protein traR
AXA51_RS23865 (AXA51_24150) 37081..37605 - 525 WP_022649995 conjugal transfer protein TraQ traQ
AXA51_RS23870 (AXA51_24155) 37605..38378 - 774 WP_123839398 conjugal transfer protein TraP traP
AXA51_RS23875 (AXA51_24160) 38359..39666 - 1308 WP_022649993 conjugal transfer protein TraO traO
AXA51_RS23880 (AXA51_24165) 39666..40646 - 981 WP_022649992 DotH/IcmK family type IV secretion protein traN
AXA51_RS23885 (AXA51_24170) 40657..41274 - 618 WP_179297403 DotI/IcmL family type IV secretion protein traM
AXA51_RS23890 (AXA51_24175) 41364..41714 - 351 WP_022649990 hypothetical protein traL
AXA51_RS23895 (AXA51_24180) 41732..45273 - 3542 Protein_51 LPD7 domain-containing protein -
AXA51_RS23900 (AXA51_24185) 45345..45632 - 288 WP_022649988 hypothetical protein traK
AXA51_RS23905 (AXA51_24190) 45629..46789 - 1161 WP_022649987 plasmid transfer ATPase TraJ virB11
AXA51_RS23910 (AXA51_24195) 46802..47608 - 807 WP_022649986 type IV secretory system conjugative DNA transfer family protein traI
AXA51_RS23915 (AXA51_24200) 47605..48078 - 474 WP_022649985 DotD/TraH family lipoprotein -
AXA51_RS23920 48149..48565 - 417 WP_123839394 hypothetical protein -
AXA51_RS23925 (AXA51_24205) 48674..49879 - 1206 WP_022649984 conjugal transfer protein TraF -
AXA51_RS23930 (AXA51_24210) 49985..50821 - 837 WP_022649983 hypothetical protein traE
AXA51_RS23935 50968..51444 - 477 WP_022649982 hypothetical protein -
AXA51_RS23940 (AXA51_24215) 51464..52624 - 1161 WP_022649981 site-specific integrase -
AXA51_RS23945 (AXA51_24220) 53202..54539 - 1338 WP_022649980 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
AXA51_RS23950 (AXA51_24225) 54536..55210 - 675 WP_022649979 prepilin peptidase -
AXA51_RS23955 (AXA51_24230) 55195..55677 - 483 WP_045261878 lytic transglycosylase domain-containing protein virB1
AXA51_RS23960 (AXA51_24235) 55762..56376 - 615 WP_022649977 type 4 pilus major pilin -
AXA51_RS23965 (AXA51_24240) 56390..57484 - 1095 WP_032623575 type II secretion system F family protein -
AXA51_RS23970 (AXA51_24245) 57477..59036 - 1560 WP_022649975 ATPase, T2SS/T4P/T4SS family virB11
AXA51_RS23975 (AXA51_24250) 59039..59554 - 516 WP_022649974 type IV pilus biogenesis protein PilP -
AXA51_RS23980 (AXA51_24255) 59538..60842 - 1305 WP_022649973 type 4b pilus protein PilO2 -
AXA51_RS23985 (AXA51_24260) 60844..62520 - 1677 WP_022649972 PilN family type IVB pilus formation outer membrane protein -
AXA51_RS23990 (AXA51_24265) 62535..62972 - 438 WP_022649971 type IV pilus biogenesis protein PilM -

Host bacterium

ID   6198 GenBank   NZ_CP031572
Plasmid name   pQnrS1-1502262 Incompatibility group   -
Plasmid size   106689 bp Coordinate of oriT [Strand]   18385..18471 [-]
Host baterium   Enterobacter hormaechei strain N1

Cargo genes

Drug resistance gene   blaLAP-2, qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIC1, AcrIIA7