Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105760
Name   oriT1_pRW35-like in_silico
Organism   Streptococcus pyogenes strain TSPY556
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP032701 (2671..2711 [+], 41 nt)
oriT length   41 nt
IRs (inverted repeats)      15..23, 29..37  (TAAAGTATA..TATACTTTA)
 4..9, 12..17  (TTATAT..ATATAA)
Location of nic site      28..29
Conserved sequence flanking the
  nic site    		  GTGTGTATAC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 41 nt

>oriT1_pRW35-like
CACTTATATGAATATAAAGTATAGTGTGTATACTTTACATG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3900 GenBank   WP_014072567
Name   mobV_D8S77_RS09250_pRW35-like insolico UniProt ID   G3ER43
Length   500 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 500 a.a.        Molecular weight: 58463.75 Da        Isoelectric Point: 6.0256

>WP_014072567.1 MULTISPECIES: MobV family relaxase [Streptococcus]
MSYLVARMQKMKVGNLGGAYRHNERVFENHSNKDIDTSRSHLNYELTDRDRSVSYEKQIKDYVNEKKSSK
RAIRKDAVLCDEWIITSDKDFFERLDQEQTRAFFETAKNYFAERYGLENIAYASVHLDESTPHMHMGVVP
MVDGKLSSKAVFTREELKAIQEELPKYMNEQGFELSRGQLGSDKKHLSVADYKAKIGKEALNKELLGLGA
PRYWHKEEDRPATAEEIAGYESLASLFAGEEMKLREATLEERFTWLDSHRNDLKGDLSHLEELVDKKIEE
YTRIDSEASERLSELSELNSKVKDREKELRGLESDSERLSDKVVRLEKEHRETTQLLVEQNRNLRKISFQ
DLDRRRIAEDLHEELEKATPKLFGGSFNFTADFVGRLKTFMSEVVEKLEQVINQNEVLRKALEGMKQAKE
RAERELLQEEWKTQRLETENQNLRQENKELKVSKNLLEDIQEVITEKEVSSLNKRLDELREARMASKRGY
EPKRSKGRSI

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-194)


  Protein structure


Source ID Structure
AlphaFold DB G3ER43


Host bacterium

ID   6197 GenBank   NZ_CP032701
Plasmid name   pRW35-like Incompatibility group   -
Plasmid size   4968 bp Coordinate of oriT [Strand]   2671..2711 [+]; 1344..1381 [+]
Host baterium   Streptococcus pyogenes strain TSPY556

Cargo genes

Drug resistance gene   erm(T)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -