Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105756
Name   oriT_p3-20710 in_silico
Organism   Enterobacter hormaechei strain 20710
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP030077 (83867..84017 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_p3-20710
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3896 GenBank   WP_001337757
Name   mobH_DPF84_RS24635_p3-20710 insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   3909 GenBank   WP_000178856
Name   traD_DPF84_RS24640_p3-20710 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   3910 GenBank   WP_000637386
Name   traC_DPF84_RS24715_p3-20710 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 81951..107142

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPF84_RS24625 (DPF84_24620) 77976..78209 - 234 WP_001191892 hypothetical protein -
DPF84_RS24630 (DPF84_24625) 78191..78808 - 618 WP_001249396 hypothetical protein -
DPF84_RS24635 (DPF84_24630) 78976..81954 + 2979 WP_001337757 MobH family relaxase -
DPF84_RS24640 (DPF84_24635) 81951..83816 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
DPF84_RS24645 (DPF84_24640) 83866..84411 + 546 WP_000228720 hypothetical protein -
DPF84_RS24650 (DPF84_24645) 84368..84997 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
DPF84_RS24655 (DPF84_24650) 85007..85453 + 447 WP_000122506 hypothetical protein -
DPF84_RS24660 (DPF84_24655) 85463..85840 + 378 WP_000869261 hypothetical protein -
DPF84_RS24665 (DPF84_24660) 85840..86502 + 663 WP_001231463 hypothetical protein -
DPF84_RS28410 86683..86826 + 144 WP_001275801 hypothetical protein -
DPF84_RS24670 (DPF84_24665) 86838..87203 + 366 WP_001052531 hypothetical protein -
DPF84_RS24675 (DPF84_24670) 87348..87629 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
DPF84_RS24680 (DPF84_24675) 87626..88252 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
DPF84_RS24685 (DPF84_24680) 88236..89153 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
DPF84_RS24690 (DPF84_24685) 89153..90466 + 1314 WP_024131605 TraB/VirB10 family protein traB
DPF84_RS24695 (DPF84_24690) 90463..91041 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
DPF84_RS24700 (DPF84_24695) 91045..91437 + 393 WP_000479535 TraA family conjugative transfer protein -
DPF84_RS24705 (DPF84_24700) 91638..97169 + 5532 WP_000606833 Ig-like domain-containing protein -
DPF84_RS24710 (DPF84_24705) 97318..98025 + 708 WP_001259347 DsbC family protein -
DPF84_RS24715 (DPF84_24710) 98022..100469 + 2448 WP_000637386 type IV secretion system protein TraC virb4
DPF84_RS24720 (DPF84_24715) 100484..100801 + 318 WP_000351984 hypothetical protein -
DPF84_RS24725 (DPF84_24720) 100798..101328 + 531 WP_001010738 S26 family signal peptidase -
DPF84_RS24730 (DPF84_24725) 101291..102556 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
DPF84_RS24735 (DPF84_24730) 102553..103224 + 672 WP_001337754 EAL domain-containing protein -
DPF84_RS24740 (DPF84_24735) 103224..104228 + 1005 WP_043940352 TraU family protein traU
DPF84_RS24745 (DPF84_24740) 104344..107142 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
DPF84_RS24750 (DPF84_24745) 107181..108041 - 861 WP_000709517 hypothetical protein -
DPF84_RS24755 (DPF84_24750) 108164..108805 - 642 WP_000796664 hypothetical protein -
DPF84_RS24760 (DPF84_24755) 109099..109419 + 321 WP_000547566 hypothetical protein -
DPF84_RS24765 (DPF84_24760) 109723..109908 + 186 WP_001186917 hypothetical protein -
DPF84_RS24770 (DPF84_24765) 110128..111096 + 969 WP_000085162 AAA family ATPase -
DPF84_RS24775 (DPF84_24770) 111107..112015 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   6193 GenBank   NZ_CP030077
Plasmid name   p3-20710 Incompatibility group   IncA/C2
Plasmid size   170955 bp Coordinate of oriT [Strand]   83867..84017 [+]
Host baterium   Enterobacter hormaechei strain 20710

Cargo genes

Drug resistance gene   catA1, aadA5, qacE, sul1, blaSED1, armA, aph(6)-Id, aph(3'')-Ib, sul2, blaTEM-1B
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -