Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105741
Name   oriT_p34998-106.409kb in_silico
Organism   Enterobacter hormaechei subsp. steigerwaltii strain 34998
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP010383 (44537..44623 [+], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_p34998-106.409kb
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3889 GenBank   WP_022650008
Name   Relaxase_LI65_RS00540_p34998-106.409kb insolico UniProt ID   A0A4Q2QTK9
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102314.37 Da        Isoelectric Point: 8.2756

>WP_022650008.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(51-317)


  Protein structure


Source ID Structure
AlphaFold DB A0A4Q2QTK9


T4CP

ID   3903 GenBank   WP_022650006
Name   trbC_LI65_RS00530_p34998-106.409kb insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87660.94 Da        Isoelectric Point: 6.8660

>WP_022650006.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 3970..38414

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI65_RS00360 (LI65_01030) 34..471 + 438 WP_022649971 type IV pilus biogenesis protein PilM -
LI65_RS00365 (LI65_00385) 486..2162 + 1677 WP_022649972 PilN family type IVB pilus formation outer membrane protein -
LI65_RS00370 (LI65_00390) 2164..3468 + 1305 WP_022649973 type 4b pilus protein PilO2 -
LI65_RS00375 (LI65_00395) 3452..3967 + 516 WP_022649974 type IV pilus biogenesis protein PilP -
LI65_RS00380 (LI65_00400) 3970..5529 + 1560 WP_022649975 ATPase, T2SS/T4P/T4SS family virB11
LI65_RS00385 (LI65_00405) 5522..6616 + 1095 WP_032623575 type II secretion system F family protein -
LI65_RS00390 (LI65_00410) 6630..7244 + 615 WP_022649977 type 4 pilus major pilin -
LI65_RS00395 (LI65_00415) 7329..7811 + 483 WP_045261878 lytic transglycosylase domain-containing protein virB1
LI65_RS00400 (LI65_00420) 7796..8470 + 675 WP_022649979 prepilin peptidase -
LI65_RS00405 (LI65_00425) 8467..9801 + 1335 WP_032627586 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
LI65_RS00410 (LI65_00435) 10382..11542 + 1161 WP_022649981 site-specific integrase -
LI65_RS27615 11562..12038 + 477 WP_022649982 hypothetical protein -
LI65_RS00415 12185..13021 + 837 WP_022649983 hypothetical protein traE
LI65_RS00420 (LI65_00450) 13127..14332 + 1206 WP_022649984 conjugal transfer protein TraF -
LI65_RS27620 14441..14857 + 417 WP_123839394 hypothetical protein -
LI65_RS00425 (LI65_00460) 14928..15401 + 474 WP_022649985 DotD/TraH family lipoprotein -
LI65_RS00430 (LI65_00465) 15398..16204 + 807 WP_022649986 type IV secretory system conjugative DNA transfer family protein traI
LI65_RS00435 (LI65_00470) 16217..17377 + 1161 WP_022649987 plasmid transfer ATPase TraJ virB11
LI65_RS00440 (LI65_00475) 17374..17661 + 288 WP_022649988 hypothetical protein traK
LI65_RS00445 (LI65_00480) 17733..21275 + 3543 WP_032635082 LPD7 domain-containing protein -
LI65_RS00450 (LI65_00485) 21293..21643 + 351 WP_022649990 hypothetical protein traL
LI65_RS00455 (LI65_00490) 21733..22350 + 618 WP_179297403 DotI/IcmL family type IV secretion protein traM
LI65_RS00460 (LI65_00495) 22361..23341 + 981 WP_022649992 DotH/IcmK family type IV secretion protein traN
LI65_RS00465 (LI65_00500) 23341..24648 + 1308 WP_022649993 conjugal transfer protein TraO traO
LI65_RS27105 (LI65_00505) 24632..25402 + 771 WP_022649994 conjugal transfer protein TraP traP
LI65_RS00475 (LI65_00510) 25402..25926 + 525 WP_022649995 conjugal transfer protein TraQ traQ
LI65_RS00480 (LI65_00515) 25961..26365 + 405 WP_022649996 DUF6750 family protein traR
LI65_RS00485 (LI65_01040) 26549..27274 + 726 WP_063864836 hypothetical protein traT
LI65_RS00490 (LI65_00525) 27361..30414 + 3054 WP_022649998 hypothetical protein traU
LI65_RS27765 (LI65_01045) 30414..30983 + 570 WP_022649999 hypothetical protein traV
LI65_RS00500 (LI65_00535) 30996..32198 + 1203 WP_022650000 conjugal transfer protein TraW traW
LI65_RS00505 (LI65_01050) 32195..32737 + 543 WP_022650001 conjugal transfer protein TraX -
LI65_RS00510 (LI65_00545) 32795..34954 + 2160 WP_022650002 DotA/TraY family protein traY
LI65_RS00515 (LI65_00550) 35016..35648 + 633 WP_022650003 plasmid IncI1-type surface exclusion protein ExcA -
LI65_RS00520 (LI65_00555) 35969..37177 + 1209 WP_022650004 hypothetical protein trbA
LI65_RS00525 37230..38414 + 1185 WP_022650005 hypothetical protein trbB
LI65_RS00530 (LI65_01060) 38418..40718 + 2301 WP_022650006 F-type conjugative transfer protein TrbC -
LI65_RS00535 (LI65_00570) 40792..41061 + 270 WP_022650007 hypothetical protein -
LI65_RS27625 41195..41398 + 204 WP_123839396 hypothetical protein -

Host bacterium

ID   6178 GenBank   NZ_CP010383
Plasmid name   p34998-106.409kb Incompatibility group   -
Plasmid size   106410 bp Coordinate of oriT [Strand]   44537..44623 [+]
Host baterium   Enterobacter hormaechei subsp. steigerwaltii strain 34998

Cargo genes

Drug resistance gene   qnrS1, blaLAP-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA7, AcrIIC1