Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105737
Name   oriT_p34983-59.134kb in_silico
Organism   Enterobacter hormaechei subsp. hormaechei strain 34983
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP010378 (51860..51949 [-], 90 nt)
oriT length   90 nt
IRs (inverted repeats)      34..41, 44..51  (AGTGCGAT..ATCGCACT)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 90 nt

>oriT_p34983-59.134kb
TATTTCATTTTTTGTTTTTTTAATCAGTTAGTTAGTGCGATTCATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3885 GenBank   WP_024561907
Name   mobF_LI64_RS22335_p34983-59.134kb insolico UniProt ID   H1ZMV6
Length   1072 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1072 a.a.        Molecular weight: 118896.59 Da        Isoelectric Point: 6.4636

>WP_024561907.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNDQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGTTEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKKRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHSDGQQEKSYGHDAGHQTEEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDHDKEGYGL

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(16-288)

AAA domain (AAA_30)

(480-656)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(855-899)


  Protein structure


Source ID Structure
AlphaFold DB H1ZMV6


T4CP

ID   3899 GenBank   WP_032493179
Name   t4cp2_LI64_RS22330_p34983-59.134kb insolico UniProt ID   _
Length   510 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 510 a.a.        Molecular weight: 57912.96 Da        Isoelectric Point: 9.5127

>WP_032493179.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVAIIAGAVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAEKTKERDHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGTDKRIVLDPNGEYVETFYRPGDVILSPYDKRSPGWSMFNEIRDDFDYDLYAVSIIQ
ESKERSAEEWYRYGRLIYREVSKKLLTLKRSPTMEEVMHWCTIVENEKLKEFVKGTPAEALFIKGAERAT
GSARFVLGDKLAPHLKMPAGDFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTYSQLIERYGINIAETLLGSMRSGIVMGSSRL
GKETLEQMSRALGEIEGEVEHKQGNPFQAGIGNRPRIETRTVRAVTPTEISELKNLTGYVYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-492)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 24796..35072

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI64_RS22110 (LI64_22110) 22759..23763 + 1005 WP_000427620 IS110-like element IS4321 family transposase -
LI64_RS22120 (LI64_22120) 24272..24784 - 513 WP_029697418 phospholipase D family protein -
LI64_RS22125 (LI64_22125) 24796..25797 - 1002 WP_001076634 ATPase, T2SS/T4P/T4SS family virB11
LI64_RS22130 (LI64_22130) 25848..26981 - 1134 WP_000101920 type IV secretion system protein VirB10 virB10
LI64_RS22135 (LI64_22135) 26981..27868 - 888 WP_000758230 TrbG/VirB9 family P-type conjugative transfer protein virB9
LI64_RS22140 (LI64_22140) 27879..28574 - 696 WP_001208352 type IV secretion system protein virB8
LI64_RS26655 28585..28716 - 132 WP_032493177 hypothetical protein -
LI64_RS22145 (LI64_22145) 28793..29830 - 1038 WP_014014948 type IV secretion system protein virB6
LI64_RS22150 (LI64_22150) 29847..30089 - 243 WP_000858958 EexN family lipoprotein -
LI64_RS22155 (LI64_22155) 30099..30800 - 702 WP_014014949 type IV secretion system protein virB5
LI64_RS22160 (LI64_22160) 30816..33389 - 2574 WP_040115085 VirB4 family type IV secretion/conjugal transfer ATPase virb4
LI64_RS22165 (LI64_22165) 33390..33707 - 318 WP_000462629 VirB3 family type IV secretion system protein virB3
LI64_RS22170 (LI64_22170) 33747..34043 - 297 WP_015060075 TrbC/VirB2 family protein virB2
LI64_RS22175 (LI64_22175) 34290..35072 - 783 WP_024562119 lytic transglycosylase domain-containing protein virB1
LI64_RS22180 (LI64_22180) 35172..35489 + 318 WP_000960955 H-NS family nucleoid-associated regulatory protein -
LI64_RS22185 (LI64_22185) 35493..35843 + 351 WP_024562120 hypothetical protein -
LI64_RS22190 (LI64_22190) 35840..36178 + 339 WP_032493175 hypothetical protein -
LI64_RS22195 (LI64_22195) 36182..36490 + 309 WP_000733764 hypothetical protein -
LI64_RS22200 (LI64_22200) 36775..36960 + 186 WP_024562121 ribbon-helix-helix domain-containing protein -
LI64_RS22205 (LI64_22205) 36957..37565 - 609 WP_000275218 recombinase family protein -
LI64_RS22210 (LI64_22210) 37752..38084 - 333 WP_032493174 hypothetical protein -

Host bacterium

ID   6174 GenBank   NZ_CP010378
Plasmid name   p34983-59.134kb Incompatibility group   IncN3
Plasmid size   59134 bp Coordinate of oriT [Strand]   51860..51949 [-]
Host baterium   Enterobacter hormaechei subsp. hormaechei strain 34983

Cargo genes

Drug resistance gene   sul1, qnrB2, qacE, dfrB3
Virulence gene   -
Metal resistance gene   merR, merT, merP, merC, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -