Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105733
Name   oriT_A449|4 in_silico
Organism   Aeromonas salmonicida subsp. salmonicida A449
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NC_009349 (150116..150245 [-], 130 nt)
oriT length   130 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 130 nt

>oriT_A449|4
GTTAACCCGTTTGATCGCGTGATGAATGCGCTAAAAAGTCGAGGCCGTAAGAACGCCCACATTCTGAGCATCTTGCAGCTGGATTGGCCTGCGTCTGAATCGATCATCCATCGCCTGAGCGACTACATCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3881 GenBank   WP_236614860
Name   mobH_ASA_RS22585_A449|4 insolico UniProt ID   _
Length   984 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 984 a.a.        Molecular weight: 107305.59 Da        Isoelectric Point: 4.7871

>WP_236614860.1 MobH family relaxase [Aeromonas salmonicida]
MDEEDIPRYPPFAKGLPVAPIDKILATQAELIEKVRNSLGFTIEDFNRLVLPVIHRYAAFVHLLPASEAH
HHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPKQRRDNEPRWRLASCFSGLLHDVGKPLADVSITDKN
GVVTWNPYFESLHDWAGRNHVDRYFIRWREKRHKRHEQFSLLAVDRIIPVATREYLSESGPLIIEAMLEA
ISGTSVNQPVTKLMLRADQESVSRDLKQNRLNVDEFSYGVPVERYVFDAIRRLVKTGKWKVNEPGAKVWH
LQQGVFIAWRNLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQEKGDRAYYRYWEVLPEMIQE
AAGSIKILMLRLESNELVFTTEPPAAVAGEVVGDVADAVIELIDPDEASDADDESASGMSESLLSAEQEA
MNALAGLGFGDAMEILGGTASDDNATQPAPVVDTTPPAKPAKGAGGRGGKNQAKKTAPAAPEQQQPEPSP
QEIAKSTPPLAVENPLQAIMDVGGGLGGLDFPFDAFVAGTSMSDEPSQALAPEPKQDVANPDNPDTPNLG
LMNQVFPNFMNDESTGDVGGMDLPPAWAIEEIPSFHAYEGEVGQPGNQFVSEELAGADAPDAKGELLTLM
ATFGEAGPILERAILPVLEGKATLGEVLCLMKGQAVILYPEGARALGAPSEILTALFHAQAIVPDPVMSG
RKVQDFNGVKAIVLTEQLSSAFIAAIKQAESLLGGYQDAFELVASPEVKKTQSKKNRGQRKTSRSESSGE
DSHAASPERAQVEEKRVIADAQLIDVSVEPPSPPVDTTPPPDKVVPRKESRGKEKVDKPIQPAPTTKPTE
GTQNLARLPPREQPKPESQQKQARVIDVGNIALPGPDLPEELSPRSIVKEETVDEALYLPPKMTPEKAIQ
MLKEMIKNRSGRWLVSAVLEEEGFLTTSDKAFEVIAGEYTGISKHVLGGTLRRAQRRPLLKQRQGKLYLE
VDKS

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(22-328)


  Protein structure



No available structure.




T4CP

ID   3896 GenBank   WP_011899407
Name   traD_ASA_RS22580_A449|4 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 68931.34 Da        Isoelectric Point: 6.8339

>WP_011899407.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTYDPLAYEMPWRPNYEMSAVFGWVAASVGALAVQQITDMPPEPFYWMTGICGVMTMARLPKAIKLHLLQ
KHLKGRDLEFISIKELQKKVQASPEDMWLGYGFAWENRHAQRVFEILKRDWTSIVGTESTTSKIVRTIRG
KKKKEVPIGQPWIHGVEPKEEHLMQPLKHTEGHSLIVGTTGSGKTRMFDTLISQAILRGEAVIIIDPKGD
KEMRDNARRACEAMGQAERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEGGGDPFKSFGWQA
LNNIAQGLILAYDRPNLIKLRRFLEGGADGLVIKAVQAYAERVMPDWEAAAAPYLEKAANGSRSKLAVAH
MAFYNNVIQPEHPSSELEGLLNMFKHDATHFSKMVSNLLPIMNMLTSGELGPLLSPDATDLSDERQITDS
AKIINNAQVAYLGLDSLTDNMVGSALGSIFLSDLTAVAGDRYNYGVNNLPVNIFVDEAAEVINDPFIQLL
NKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNVFALRIVDAETQEYIAENLPKTRLKYVMRTQ
GQNTDGSEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGKIVKGRLPILTEK

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(470-597)

AAA-like domain (AAA_10)

(168-257)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 141182..152166

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASA_RS22500 (ASA_P4G151) 136897..137994 + 1098 WP_010676219 ISAs1-like element ISAs1 family transposase -
ASA_RS24515 (ASA_P4G152) 138085..138612 + 528 WP_139027008 hypothetical protein -
ASA_RS25515 (ASA_P4G153) 139050..139478 + 429 WP_011899394 hypothetical protein -
ASA_RS22510 (ASA_P4G154) 139490..139717 + 228 WP_011899395 hypothetical protein -
ASA_RS24525 (ASA_P4G155) 139832..140347 + 516 WP_139027007 hypothetical protein -
ASA_RS22520 (ASA_P4G156) 140396..140641 + 246 WP_011899397 hypothetical protein -
ASA_RS22525 (ASA_P4G157) 141182..141463 + 282 WP_011899398 type IV conjugative transfer system protein TraL traL
ASA_RS22530 (ASA_P4G158) 141460..142095 + 636 WP_011899399 TraE/TraK family type IV conjugative transfer system protein traE
ASA_RS22535 (ASA_P4G159) 142079..142819 + 741 WP_236614858 type-F conjugative transfer system secretin TraK traK
ASA_RS22540 (ASA_P4G160) 142988..144307 + 1320 WP_139027009 TraB/VirB10 family protein traB
ASA_RS22545 (ASA_P4G161) 144304..144870 + 567 WP_011899401 type IV conjugative transfer system lipoprotein TraV traV
ASA_RS22550 (ASA_P4G162) 144961..145287 + 327 WP_050804919 TraA family conjugative transfer protein -
ASA_RS22555 (ASA_P4G163) 145469..147268 + 1800 WP_139027006 DUF4165 domain-containing protein -
ASA_RS22560 (ASA_P4G164) 147273..148370 - 1098 WP_010676219 ISAs1-like element ISAs1 family transposase -
ASA_RS22565 (ASA_P4G165) 148582..148974 - 393 WP_043774535 hypothetical protein -
ASA_RS22570 (ASA_P4G166) 149120..149749 - 630 WP_011899405 DUF4400 domain-containing protein tfc7
ASA_RS22575 (ASA_P4G167) 149706..150290 - 585 WP_011899406 hypothetical protein -
ASA_RS22580 (ASA_P4G168) 150301..152166 - 1866 WP_011899407 conjugative transfer system coupling protein TraD virb4
ASA_RS22585 (ASA_P4G169) 152169..155123 - 2955 WP_236614860 MobH family relaxase -
ASA_RS22590 (ASA_P4G170) 155373..155987 + 615 WP_011899409 hypothetical protein -

Host bacterium

ID   6170 GenBank   NC_009349
Plasmid name   A449|4 Incompatibility group   -
Plasmid size   166749 bp Coordinate of oriT [Strand]   150116..150245 [-]
Host baterium   Aeromonas salmonicida subsp. salmonicida A449

Cargo genes

Drug resistance gene   tet(E), sul1, qacE, ant(3'')-Ia, cat
Virulence gene   hcp
Metal resistance gene   merR, merT, merP, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -