Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105729
Name   oriT_p7994F in_silico
Organism   Enterobacter hormaechei subsp. steigerwaltii strain NMI7994_17
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MZ855469 (68417..68498 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_p7994F
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3878 GenBank   WP_015632446
Name   Relaxase_LN376_RS27120_p7994F insolico UniProt ID   R4WBX2
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74631.63 Da        Isoelectric Point: 9.3060

>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB R4WBX2


T4CP

ID   3893 GenBank   WP_015632442
Name   trbC_LN376_RS27155_p7994F insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82688.74 Da        Isoelectric Point: 5.8919

>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(442-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 76359..98200

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN376_RS27135 71419..71826 + 408 WP_015632445 transposase -
LN376_RS27140 71823..72172 + 350 Protein_88 IS66 family insertion sequence element accessory protein TnpB -
LN376_RS27145 72202..73791 + 1590 WP_015632444 IS66 family transposase -
LN376_RS27150 73814..74119 - 306 WP_015632443 phospholipase D-like domain-containing protein -
LN376_RS27155 74165..76357 - 2193 WP_015632442 F-type conjugative transfer protein TrbC -
LN376_RS27160 76359..77513 - 1155 WP_015632441 thioredoxin fold domain-containing protein trbB
LN376_RS27165 77461..78879 - 1419 WP_015632440 hypothetical protein trbA
LN376_RS27170 78981..79577 - 597 WP_123677147 plasmid IncI1-type surface exclusion protein ExcA -
LN376_RS27175 79670..81814 - 2145 WP_015632438 DotA/TraY family protein traY
LN376_RS27180 81856..82437 - 582 WP_015632437 conjugal transfer protein TraX -
LN376_RS27185 82434..83630 - 1197 WP_015632436 conjugal transfer protein TraW traW
LN376_RS27190 83641..86688 - 3048 WP_015632435 conjugal transfer protein traU
LN376_RS27195 86698..87261 - 564 WP_032495746 hypothetical protein traT
LN376_RS27200 87305..87706 - 402 WP_015632433 DUF6750 family protein traR
LN376_RS27205 87760..88293 - 534 WP_015632432 conjugal transfer protein TraQ traQ
LN376_RS27210 88303..89022 - 720 WP_015632431 conjugal transfer protein TraP traP
LN376_RS27215 89028..90368 - 1341 WP_015632430 conjugal transfer protein TraO traO
LN376_RS27220 90372..91475 - 1104 WP_015632429 DotH/IcmK family type IV secretion protein traN
LN376_RS27225 91486..92190 - 705 WP_015632428 DotI/IcmL/TraM family protein traM
LN376_RS27230 92226..92579 - 354 WP_015632427 hypothetical protein traL
LN376_RS27235 92613..95936 - 3324 WP_032495745 LPD7 domain-containing protein -
LN376_RS27240 95954..96220 - 267 WP_032441926 IcmT/TraK family protein traK
LN376_RS27245 96217..97404 - 1188 WP_032495744 plasmid transfer ATPase TraJ virB11
LN376_RS27250 97382..98200 - 819 WP_015632425 type IV secretory system conjugative DNA transfer family protein traI
LN376_RS27255 98197..98664 - 468 WP_015632424 DotD/TraH family lipoprotein -
LN376_RS27260 98687..99166 - 480 WP_015632423 lytic transglycosylase domain-containing protein -
LN376_RS27265 99179..99784 - 606 WP_015632422 hypothetical protein -
LN376_RS27270 99864..100175 - 312 WP_015632421 hypothetical protein -
LN376_RS27275 100209..100412 - 204 WP_032495743 hypothetical protein -
LN376_RS27280 100430..100897 - 468 WP_032495742 transcription termination/antitermination NusG family protein -
LN376_RS27285 101231..101809 + 579 WP_015632419 hypothetical protein -
LN376_RS27290 101791..102261 + 471 WP_032441927 hypothetical protein -
LN376_RS27295 102261..102647 + 387 WP_015632417 acyl-CoA thioesterase -

Host bacterium

ID   6166 GenBank   NZ_MZ855469
Plasmid name   p7994F Incompatibility group   IncFII
Plasmid size   103126 bp Coordinate of oriT [Strand]   68417..68498 [+]
Host baterium   Enterobacter hormaechei subsp. steigerwaltii strain NMI7994_17

Cargo genes

Drug resistance gene   blaNDM-1, aac(6')-Ib-cr, blaOXA-1, blaCTX-M-15, aac(6')-Ib
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -