Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105720
Name   oriT_p1799F in_silico
Organism   Enterobacter hormaechei subsp. xiangfangensis strain NMI1799_20
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MZ750396 (71835..71916 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_p1799F
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3872 GenBank   WP_015632446
Name   Relaxase_LN394_RS26845_p1799F insolico UniProt ID   R4WBX2
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74631.63 Da        Isoelectric Point: 9.3060

>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB R4WBX2


T4CP

ID   3884 GenBank   WP_015632442
Name   trbC_LN394_RS26880_p1799F insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82688.74 Da        Isoelectric Point: 5.8919

>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(442-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 79777..101618

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN394_RS26860 74837..75244 + 408 WP_015632445 transposase -
LN394_RS26865 75241..75590 + 350 Protein_90 IS66 family insertion sequence element accessory protein TnpB -
LN394_RS26870 75620..77209 + 1590 WP_015632444 IS66 family transposase -
LN394_RS26875 77232..77537 - 306 WP_015632443 phospholipase D-like domain-containing protein -
LN394_RS26880 77583..79775 - 2193 WP_015632442 F-type conjugative transfer protein TrbC -
LN394_RS26885 79777..80931 - 1155 WP_015632441 thioredoxin fold domain-containing protein trbB
LN394_RS26890 80879..82297 - 1419 WP_015632440 hypothetical protein trbA
LN394_RS26895 82399..82995 - 597 WP_123677147 plasmid IncI1-type surface exclusion protein ExcA -
LN394_RS26900 83088..85232 - 2145 WP_015632438 DotA/TraY family protein traY
LN394_RS26905 85274..85855 - 582 WP_015632437 conjugal transfer protein TraX -
LN394_RS26910 85852..87048 - 1197 WP_015632436 conjugal transfer protein TraW traW
LN394_RS26915 87059..90106 - 3048 WP_015632435 conjugal transfer protein traU
LN394_RS26920 90116..90679 - 564 WP_032495746 hypothetical protein traT
LN394_RS26925 90723..91124 - 402 WP_015632433 DUF6750 family protein traR
LN394_RS26930 91178..91711 - 534 WP_015632432 conjugal transfer protein TraQ traQ
LN394_RS26935 91721..92440 - 720 WP_015632431 conjugal transfer protein TraP traP
LN394_RS26940 92446..93786 - 1341 WP_015632430 conjugal transfer protein TraO traO
LN394_RS26945 93790..94893 - 1104 WP_015632429 DotH/IcmK family type IV secretion protein traN
LN394_RS26950 94904..95608 - 705 WP_015632428 DotI/IcmL/TraM family protein traM
LN394_RS26955 95644..95997 - 354 WP_015632427 hypothetical protein traL
LN394_RS26960 96031..99354 - 3324 WP_032495745 LPD7 domain-containing protein -
LN394_RS26965 99372..99638 - 267 WP_032441926 IcmT/TraK family protein traK
LN394_RS26970 99635..100822 - 1188 WP_032495744 plasmid transfer ATPase TraJ virB11
LN394_RS26975 100800..101618 - 819 WP_015632425 type IV secretory system conjugative DNA transfer family protein traI
LN394_RS26980 101615..102082 - 468 WP_015632424 DotD/TraH family lipoprotein -
LN394_RS26985 102105..102584 - 480 WP_015632423 lytic transglycosylase domain-containing protein -
LN394_RS26990 102597..103202 - 606 WP_015632422 hypothetical protein -
LN394_RS26995 103282..103593 - 312 WP_015632421 hypothetical protein -
LN394_RS27000 103627..103830 - 204 WP_032495743 hypothetical protein -
LN394_RS27005 103848..104315 - 468 WP_032495742 transcription termination/antitermination NusG family protein -
LN394_RS27010 104649..105227 + 579 WP_015632419 hypothetical protein -
LN394_RS27015 105209..105679 + 471 WP_032441927 hypothetical protein -
LN394_RS27020 105679..106065 + 387 WP_015632417 acyl-CoA thioesterase -

Host bacterium

ID   6157 GenBank   NZ_MZ750396
Plasmid name   p1799F Incompatibility group   IncFII
Plasmid size   106544 bp Coordinate of oriT [Strand]   71835..71916 [+]
Host baterium   Enterobacter hormaechei subsp. xiangfangensis strain NMI1799_20

Cargo genes

Drug resistance gene   blaNDM-1, aac(6')-Ib-cr, blaOXA-1, aac(3)-IIa, blaCTX-M-15, aac(6')-Ib
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -