Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105719 |
| Name | oriT_p5331F  |
| Organism | Enterobacter hormaechei subsp. xiangfangensis strain NMI5331_19 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MZ750398 (68383..68464 [+], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | 27..32, 35..40 (GCCACC..GGTGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_p5331F
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3871 | GenBank | WP_015632446 |
| Name | Relaxase_LN392_RS25655_p5331F |
UniProt ID | R4WBX2 |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74631.63 Da Isoelectric Point: 9.3060
>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R4WBX2 |
T4CP
| ID | 3883 | GenBank | WP_015632442 |
| Name | trbC_LN392_RS25690_p5331F |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82688.74 Da Isoelectric Point: 5.8919
>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 76325..98166
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LN392_RS25670 | 71385..71792 | + | 408 | WP_015632445 | transposase | - |
| LN392_RS25675 | 71789..72138 | + | 350 | Protein_87 | IS66 family insertion sequence element accessory protein TnpB | - |
| LN392_RS25680 | 72168..73757 | + | 1590 | WP_015632444 | IS66 family transposase | - |
| LN392_RS25685 | 73780..74085 | - | 306 | WP_015632443 | phospholipase D-like domain-containing protein | - |
| LN392_RS25690 | 74131..76323 | - | 2193 | WP_015632442 | F-type conjugative transfer protein TrbC | - |
| LN392_RS25695 | 76325..77479 | - | 1155 | WP_015632441 | thioredoxin fold domain-containing protein | trbB |
| LN392_RS25700 | 77427..78845 | - | 1419 | WP_015632440 | hypothetical protein | trbA |
| LN392_RS25705 | 78947..79543 | - | 597 | WP_123677147 | plasmid IncI1-type surface exclusion protein ExcA | - |
| LN392_RS25710 | 79636..81780 | - | 2145 | WP_015632438 | DotA/TraY family protein | traY |
| LN392_RS25715 | 81822..82403 | - | 582 | WP_015632437 | conjugal transfer protein TraX | - |
| LN392_RS25720 | 82400..83596 | - | 1197 | WP_015632436 | conjugal transfer protein TraW | traW |
| LN392_RS25725 | 83607..86654 | - | 3048 | WP_015632435 | conjugal transfer protein | traU |
| LN392_RS25730 | 86664..87227 | - | 564 | WP_032495746 | hypothetical protein | traT |
| LN392_RS25735 | 87271..87672 | - | 402 | WP_015632433 | DUF6750 family protein | traR |
| LN392_RS25740 | 87726..88259 | - | 534 | WP_015632432 | conjugal transfer protein TraQ | traQ |
| LN392_RS25745 | 88269..88988 | - | 720 | WP_015632431 | conjugal transfer protein TraP | traP |
| LN392_RS25750 | 88994..90334 | - | 1341 | WP_015632430 | conjugal transfer protein TraO | traO |
| LN392_RS25755 | 90338..91441 | - | 1104 | WP_015632429 | DotH/IcmK family type IV secretion protein | traN |
| LN392_RS25760 | 91452..92156 | - | 705 | WP_015632428 | DotI/IcmL/TraM family protein | traM |
| LN392_RS25765 | 92192..92545 | - | 354 | WP_015632427 | hypothetical protein | traL |
| LN392_RS25770 | 92579..95902 | - | 3324 | WP_032495745 | LPD7 domain-containing protein | - |
| LN392_RS25775 | 95920..96186 | - | 267 | WP_032441926 | IcmT/TraK family protein | traK |
| LN392_RS25780 | 96183..97370 | - | 1188 | WP_032495744 | plasmid transfer ATPase TraJ | virB11 |
| LN392_RS25785 | 97348..98166 | - | 819 | WP_015632425 | type IV secretory system conjugative DNA transfer family protein | traI |
| LN392_RS25790 | 98163..98630 | - | 468 | WP_015632424 | DotD/TraH family lipoprotein | - |
| LN392_RS25795 | 98653..99132 | - | 480 | WP_015632423 | lytic transglycosylase domain-containing protein | - |
| LN392_RS25800 | 99145..99750 | - | 606 | WP_015632422 | hypothetical protein | - |
| LN392_RS25805 | 99830..100141 | - | 312 | WP_015632421 | hypothetical protein | - |
| LN392_RS25810 | 100175..100378 | - | 204 | WP_032495743 | hypothetical protein | - |
| LN392_RS25815 | 100396..100863 | - | 468 | WP_032495742 | transcription termination/antitermination NusG family protein | - |
| LN392_RS25820 | 101197..101775 | + | 579 | WP_015632419 | hypothetical protein | - |
| LN392_RS25825 | 101757..102227 | + | 471 | WP_032441927 | hypothetical protein | - |
| LN392_RS25830 | 102227..102613 | + | 387 | WP_015632417 | acyl-CoA thioesterase | - |
Host bacterium
| ID | 6156 | GenBank | NZ_MZ750398 |
| Plasmid name | p5331F | Incompatibility group | IncFII |
| Plasmid size | 103092 bp | Coordinate of oriT [Strand] | 68383..68464 [+] |
| Host baterium | Enterobacter hormaechei subsp. xiangfangensis strain NMI5331_19 |
Cargo genes
| Drug resistance gene | blaNDM-1, aac(6')-Ib-cr, blaOXA-1, blaCTX-M-15, aac(6')-Ib |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |