Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105713
Name   oriT_p1_C-5-1 in_silico
Organism   Edwardsiella anguillarum strain C-5-1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP095164 (80827..80977 [-], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_p1_C-5-1
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3867 GenBank   WP_001337757
Name   mobH_MUN71_RS19575_p1_C-5-1 insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   3879 GenBank   WP_000637386
Name   traC_MUN71_RS19490_p1_C-5-1 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.



ID   3880 GenBank   WP_000178856
Name   traD_MUN71_RS19570_p1_C-5-1 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 62288..82893

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUN71_RS19450 (MUN71_19450) 58702..59088 - 387 WP_001077335 hypothetical protein -
MUN71_RS19455 (MUN71_19455) 59546..60151 - 606 Protein_63 NgrC -
MUN71_RS19460 (MUN71_19460) 60236..61258 + 1023 WP_000627495 IS21-like element ISSen3 family transposase -
MUN71_RS19465 (MUN71_19465) 61255..62037 + 783 WP_001531258 IS21-like element ISSen3 family helper ATPase IstB -
MUN71_RS19470 (MUN71_19470) 62103..62291 - 189 Protein_66 EAL domain-containing protein -
MUN71_RS19475 (MUN71_19475) 62288..63553 - 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
MUN71_RS19480 (MUN71_19480) 63516..64046 - 531 WP_001010738 S26 family signal peptidase -
MUN71_RS19485 (MUN71_19485) 64043..64360 - 318 WP_000351984 hypothetical protein -
MUN71_RS19490 (MUN71_19490) 64375..66822 - 2448 WP_000637386 type IV secretion system protein TraC virb4
MUN71_RS19495 (MUN71_19495) 66819..67526 - 708 WP_001259347 DsbC family protein -
MUN71_RS19500 (MUN71_19500) 67675..73206 - 5532 WP_000606833 Ig-like domain-containing protein -
MUN71_RS19505 (MUN71_19505) 73407..73799 - 393 WP_000479535 TraA family conjugative transfer protein -
MUN71_RS19510 (MUN71_19510) 73803..74381 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
MUN71_RS19515 (MUN71_19515) 74378..75691 - 1314 WP_024131605 TraB/VirB10 family protein traB
MUN71_RS19520 (MUN71_19520) 75691..76608 - 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
MUN71_RS19525 (MUN71_19525) 76592..77218 - 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
MUN71_RS19530 (MUN71_19530) 77215..77496 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
MUN71_RS19535 (MUN71_19535) 77641..78006 - 366 WP_001052531 hypothetical protein -
MUN71_RS19540 (MUN71_19540) 78018..78161 - 144 WP_001275801 hypothetical protein -
MUN71_RS19545 (MUN71_19545) 78342..79004 - 663 WP_001231463 hypothetical protein -
MUN71_RS19550 (MUN71_19550) 79004..79381 - 378 WP_000869261 hypothetical protein -
MUN71_RS19555 (MUN71_19555) 79391..79837 - 447 WP_000122506 hypothetical protein -
MUN71_RS19560 (MUN71_19560) 79847..80476 - 630 WP_000743450 DUF4400 domain-containing protein tfc7
MUN71_RS19565 (MUN71_19565) 80433..81017 - 585 WP_000332866 hypothetical protein -
MUN71_RS19570 (MUN71_19570) 81028..82893 - 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
MUN71_RS19575 (MUN71_19575) 82890..85868 - 2979 WP_001337757 MobH family relaxase -
MUN71_RS19580 (MUN71_19580) 86036..86653 + 618 WP_001249395 hypothetical protein -
MUN71_RS19585 (MUN71_19585) 86635..86868 + 234 WP_080783718 hypothetical protein -

Host bacterium

ID   6150 GenBank   NZ_CP095164
Plasmid name   p1_C-5-1 Incompatibility group   IncA/C2
Plasmid size   152284 bp Coordinate of oriT [Strand]   80827..80977 [-]
Host baterium   Edwardsiella anguillarum strain C-5-1

Cargo genes

Drug resistance gene   dfrA12, aadA2, qacE, sul1, mph(A), sul2, floR, tet(D), aph(3')-Ia
Virulence gene   -
Metal resistance gene   merE, merD, merB, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -