Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 105710
• Name: oriT_pYUSHP2-5
• Organism: Enterobacter hormaechei strain SH19PTE2
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP073776 (2207..2240 [-], 34 nt)
• oriT length: 34 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 34 nt

>oriT_pYUSHP2-5
ACGATTTTGCGAAGCAAAGTCTAGTGAGTACACT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_KFO54_RS25390_pYUSHP2-5

• ID: 3865
• GenBank: WP_000024818
• Name: mobV_KFO54_RS25390_pYUSHP2-5
• UniProt ID: A0A5W5SJI7
• Length: 322 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 322 a.a.
• Molecular weight: 36743.68 Da
• Isoelectric Point: 10.3759

>WP_000024818.1 MULTISPECIES: MobV family relaxase [Gammaproteobacteria]
MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCSVSKSTDQAMGRVRELLPEKRRKDAV
LAVEYVMTASPEWWKEATPRQQAEFFARSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLS
AKEFIGGRSKMREDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRK
GIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQ
MTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-185)

Protein structure

• AlphaFold DB: A0A5W5SJI7

Host bacterium

• ID: 6147
• GenBank: NZ_CP073776
• Plasmid name: pYUSHP2-5
• Incompatibility group: -
• Plasmid size: 2460 bp
• Coordinate of oriT [Strand]: 2207..2240 [-]
• Host baterium: Enterobacter hormaechei strain SH19PTE2

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -