Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105697 |
| Name | oriT_pRIVM_C019181_1  |
| Organism | Enterobacter hormaechei strain RIVM_C019181 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP071016 (273210..273464 [+], 255 nt) |
| oriT length | 255 nt |
| IRs (inverted repeats) | 153..158, 160..165 (AAAAGT..ACTTTT) 122..130, 135..143 (TTAAGGCTT..AAGCCTTAA) 49..55, 60..66 (GAATTTT..AAAATTC) |
| Location of nic site | 78..79 |
| Conserved sequence flanking the nic site |
TTTGGTTAAA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 255 nt
>oriT_pRIVM_C019181_1
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3855 | GenBank | WP_012477394 |
| Name | mobH_JQL71_RS23765_pRIVM_C019181_1 |
UniProt ID | A0A2Z2E7B6 |
| Length | 1050 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1050 a.a. Molecular weight: 118067.47 Da Isoelectric Point: 4.6347
>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z2E7B6 |
T4CP
| ID | 3866 | GenBank | WP_236904384 |
| Name | t4cp2_JQL71_RS23750_pRIVM_C019181_1 |
UniProt ID | _ |
| Length | 362 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 362 a.a. Molecular weight: 40108.90 Da Isoelectric Point: 6.2444
>WP_236904384.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacter cloacae complex]
MSIVNLTVKVMESCYARYYGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLI
DYSMSDPEHMAKMTAGIMPVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDT
AAAISQLIMSDLTSCAGSRYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTIS
DFIAAASVETANRITGLCNNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISE
RKQTTLEESIPKDLLGQVPMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLD
IKNLNKFLRKLH
MSIVNLTVKVMESCYARYYGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLI
DYSMSDPEHMAKMTAGIMPVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDT
AAAISQLIMSDLTSCAGSRYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTIS
DFIAAASVETANRITGLCNNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISE
RKQTTLEESIPKDLLGQVPMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLD
IKNLNKFLRKLH
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 3867 | GenBank | WP_241102379 |
| Name | t4cp1_JQL71_RS23760_pRIVM_C019181_1 |
UniProt ID | _ |
| Length | 344 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 344 a.a. Molecular weight: 39355.54 Da Isoelectric Point: 8.8550
>WP_241102379.1 ATP-binding protein [Enterobacter hormaechei]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHIDLPPRLDTTLS
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHIDLPPRLDTTLS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 147022..156229
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JQL71_RS23105 (JQL71_23105) | 144312..144473 | - | 162 | WP_001371949 | hypothetical protein | - |
| JQL71_RS23110 (JQL71_23110) | 147022..149703 | - | 2682 | WP_000387412 | TraC family protein | virb4 |
| JQL71_RS23115 (JQL71_23115) | 149712..150662 | - | 951 | WP_001022588 | IncHI-type conjugal transfer lipoprotein TrhV | traV |
| JQL71_RS23120 (JQL71_23120) | 150672..151424 | - | 753 | WP_182911723 | protein-disulfide isomerase HtdT | - |
| JQL71_RS23125 (JQL71_23125) | 151543..152025 | - | 483 | WP_000377632 | hypothetical protein | - |
| JQL71_RS23130 (JQL71_23130) | 152033..153388 | - | 1356 | WP_000351841 | IncHI-type conjugal transfer protein TrhB | traB |
| JQL71_RS23135 (JQL71_23135) | 153378..153839 | - | 462 | WP_021242895 | plasmid transfer protein HtdO | - |
| JQL71_RS23140 (JQL71_23140) | 153841..155112 | - | 1272 | WP_000592092 | type-F conjugative transfer system secretin TraK | traK |
| JQL71_RS23145 (JQL71_23145) | 155112..155900 | - | 789 | WP_000783153 | TraE/TraK family type IV conjugative transfer system protein | traE |
| JQL71_RS23150 (JQL71_23150) | 155912..156262 | - | 351 | WP_001447770 | type IV conjugative transfer system protein TraL | - |
| JQL71_RS23155 (JQL71_23155) | 156280..156633 | - | 354 | WP_000423602 | hypothetical protein | - |
| JQL71_RS23160 (JQL71_23160) | 157782..158657 | - | 876 | WP_000594611 | RepB family plasmid replication initiator protein | - |
| JQL71_RS23165 (JQL71_23165) | 159134..160186 | + | 1053 | WP_001065778 | hypothetical protein | - |
| JQL71_RS23170 (JQL71_23170) | 160206..160406 | + | 201 | WP_000594930 | hypothetical protein | - |
| JQL71_RS23175 (JQL71_23175) | 160403..161119 | + | 717 | WP_000838219 | zinc finger-like domain-containing protein | - |
Host bacterium
| ID | 6134 | GenBank | NZ_CP071016 |
| Plasmid name | pRIVM_C019181_1 | Incompatibility group | IncHI2A |
| Plasmid size | 316079 bp | Coordinate of oriT [Strand] | 273210..273464 [+] |
| Host baterium | Enterobacter hormaechei strain RIVM_C019181 |
Cargo genes
| Drug resistance gene | mcr-9, mph(E), msr(E), blaACC-1, blaVIM-1, ant(3'')-Ia, qacE, sul1 |
| Virulence gene | - |
| Metal resistance gene | rcnR/yohL, ncrC, pcoE, pcoS, silP, silA, silB, silF, silC, silR, silS, silE, terE, terD, terC, terB, terA, terZ, terW, merE, merD, merA, merP, merT, merR, arsH, arsR, arsB, arsC, fecE, fecD |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |