Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105692
Name   oriT_pEcl10-2 in_silico
Organism   Enterobacter hormaechei strain Eho-10
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP048705 (55910..55996 [-], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_pEcl10-2
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3852 GenBank   WP_022650008
Name   Relaxase_GVI71_RS23420_pEcl10-2 insolico UniProt ID   A0A4Q2QTK9
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102314.37 Da        Isoelectric Point: 8.2756

>WP_022650008.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(51-317)


  Protein structure


Source ID Structure
AlphaFold DB A0A4Q2QTK9


T4CP

ID   3861 GenBank   WP_022650006
Name   trbC_GVI71_RS23435_pEcl10-2 insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87660.94 Da        Isoelectric Point: 6.8660

>WP_022650006.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 62119..96563

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI71_RS23425 (GVI71_23395) 59135..59338 - 204 WP_123839396 hypothetical protein -
GVI71_RS23430 (GVI71_23400) 59472..59741 - 270 WP_022650007 hypothetical protein -
GVI71_RS23435 (GVI71_23405) 59815..62115 - 2301 WP_022650006 F-type conjugative transfer protein TrbC -
GVI71_RS23440 (GVI71_23410) 62119..63375 - 1257 WP_126530067 hypothetical protein trbB
GVI71_RS23445 (GVI71_23415) 63356..64564 - 1209 WP_022650004 hypothetical protein trbA
GVI71_RS23450 (GVI71_23420) 64885..65517 - 633 WP_022650003 plasmid IncI1-type surface exclusion protein ExcA -
GVI71_RS23455 (GVI71_23425) 65579..67738 - 2160 WP_022650002 DotA/TraY family protein traY
GVI71_RS23460 (GVI71_23430) 67796..68338 - 543 WP_022650001 conjugal transfer protein TraX -
GVI71_RS23465 (GVI71_23435) 68335..69537 - 1203 WP_022650000 conjugal transfer protein TraW traW
GVI71_RS23470 (GVI71_23440) 69550..70122 - 573 WP_154590113 conjugal transfer protein TraV traV
GVI71_RS23475 (GVI71_23445) 70119..73172 - 3054 WP_022649998 hypothetical protein traU
GVI71_RS23480 (GVI71_23450) 73259..73984 - 726 WP_063864836 hypothetical protein traT
GVI71_RS23485 (GVI71_23455) 74168..74572 - 405 WP_022649996 DUF6750 family protein traR
GVI71_RS23490 (GVI71_23460) 74607..75131 - 525 WP_022649995 conjugal transfer protein TraQ traQ
GVI71_RS23495 (GVI71_23465) 75131..75904 - 774 WP_123839398 conjugal transfer protein TraP traP
GVI71_RS23500 (GVI71_23470) 75885..77192 - 1308 WP_022649993 conjugal transfer protein TraO traO
GVI71_RS23505 (GVI71_23475) 77192..78172 - 981 WP_022649992 DotH/IcmK family type IV secretion protein traN
GVI71_RS23510 (GVI71_23480) 78183..78800 - 618 WP_179297403 DotI/IcmL family type IV secretion protein traM
GVI71_RS23515 (GVI71_23485) 78890..79240 - 351 WP_022649990 hypothetical protein traL
GVI71_RS23520 (GVI71_23490) 79258..82800 - 3543 WP_032635082 LPD7 domain-containing protein -
GVI71_RS23525 (GVI71_23495) 82872..83159 - 288 WP_022649988 hypothetical protein traK
GVI71_RS23530 (GVI71_23500) 83156..84316 - 1161 WP_022649987 plasmid transfer ATPase TraJ virB11
GVI71_RS23535 (GVI71_23505) 84329..85135 - 807 WP_022649986 type IV secretory system conjugative DNA transfer family protein traI
GVI71_RS23540 (GVI71_23510) 85132..85605 - 474 WP_022649985 DotD/TraH family lipoprotein -
GVI71_RS23545 (GVI71_23515) 85676..86092 - 417 WP_123839394 hypothetical protein -
GVI71_RS23550 (GVI71_23520) 86201..87406 - 1206 WP_022649984 conjugal transfer protein TraF -
GVI71_RS23555 (GVI71_23525) 87512..88348 - 837 WP_022649983 hypothetical protein traE
GVI71_RS23560 (GVI71_23530) 88495..88971 - 477 WP_022649982 hypothetical protein -
GVI71_RS23565 (GVI71_23535) 88991..90151 - 1161 WP_022649981 site-specific integrase -
GVI71_RS23570 (GVI71_23540) 90729..92066 - 1338 WP_040110252 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
GVI71_RS23575 (GVI71_23545) 92063..92737 - 675 WP_022649979 prepilin peptidase -
GVI71_RS23580 (GVI71_23550) 92722..93204 - 483 WP_045261878 lytic transglycosylase domain-containing protein virB1
GVI71_RS23585 (GVI71_23555) 93289..93903 - 615 WP_022649977 type 4 pilus major pilin -
GVI71_RS23590 (GVI71_23560) 93917..95011 - 1095 WP_032623575 type II secretion system F family protein -
GVI71_RS23595 (GVI71_23565) 95004..96563 - 1560 WP_022649975 ATPase, T2SS/T4P/T4SS family virB11
GVI71_RS23600 (GVI71_23570) 96566..97081 - 516 WP_022649974 type IV pilus biogenesis protein PilP -
GVI71_RS23605 (GVI71_23575) 97065..98369 - 1305 WP_022649973 type 4b pilus protein PilO2 -
GVI71_RS23610 (GVI71_23580) 98371..100047 - 1677 WP_022649972 PilN family type IVB pilus formation outer membrane protein -
GVI71_RS23615 (GVI71_23585) 100062..100499 - 438 WP_022649971 type IV pilus biogenesis protein PilM -

Host bacterium

ID   6129 GenBank   NZ_CP048705
Plasmid name   pEcl10-2 Incompatibility group   -
Plasmid size   106698 bp Coordinate of oriT [Strand]   55910..55996 [-]
Host baterium   Enterobacter hormaechei strain Eho-10

Cargo genes

Drug resistance gene   blaLAP-2, qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIC1, AcrIIA7