Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105688
Name   oriT_IR5283|unnamed2 in_silico
Organism   Enterobacter hormaechei strain IR5283
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP064115 (4109..4214 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_IR5283|unnamed2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3848 GenBank   WP_004187323
Name   Relaxase_ISU86_RS22360_IR5283|unnamed2 insolico UniProt ID   A0A3U8KUL3
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75179.89 Da        Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A3U8KUL3


T4CP

ID   3855 GenBank   WP_004206885
Name   t4cp2_ISU86_RS22475_IR5283|unnamed2 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79538.91 Da        Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 7010..34353

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU86_RS22340 (ISU86_22325) 2095..2406 + 312 WP_004187333 hypothetical protein -
ISU86_RS22345 (ISU86_22330) 2539..3075 + 537 WP_036970776 hypothetical protein -
ISU86_RS22350 (ISU86_22335) 3576..3941 - 366 WP_015586046 hypothetical protein -
ISU86_RS22355 (ISU86_22340) 4216..4533 + 318 WP_011091068 plasmid mobilization protein MobA -
ISU86_RS22360 (ISU86_22345) 4520..6499 + 1980 WP_004187323 TraI/MobA(P) family conjugative relaxase -
ISU86_RS22365 (ISU86_22350) 6513..7013 + 501 WP_004187320 DotD/TraH family lipoprotein -
ISU86_RS22370 (ISU86_22355) 7010..7789 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
ISU86_RS22375 (ISU86_22360) 7800..8963 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
ISU86_RS22380 (ISU86_22365) 8953..9213 + 261 WP_004187310 IcmT/TraK family protein traK
ISU86_RS23355 9238..12675 + 3438 WP_015586048 LPD7 domain-containing protein -
ISU86_RS22390 (ISU86_22375) 12641..13153 + 513 WP_011091071 hypothetical protein traL
ISU86_RS22395 (ISU86_22380) 13154..13366 - 213 WP_305953721 Hha/YmoA family nucleoid-associated regulatory protein -
ISU86_RS22400 (ISU86_22385) 13338..13748 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
ISU86_RS22405 (ISU86_22390) 13816..14529 + 714 WP_004187467 DotI/IcmL family type IV secretion protein traM
ISU86_RS22410 (ISU86_22395) 14538..15689 + 1152 WP_004187471 DotH/IcmK family type IV secretion protein traN
ISU86_RS22415 (ISU86_22400) 15701..17050 + 1350 WP_004187474 conjugal transfer protein TraO traO
ISU86_RS22420 (ISU86_22405) 17062..17766 + 705 WP_015060002 conjugal transfer protein TraP traP
ISU86_RS22425 (ISU86_22410) 17790..18320 + 531 WP_004187478 conjugal transfer protein TraQ traQ
ISU86_RS22430 (ISU86_22415) 18337..18726 + 390 WP_004187479 DUF6750 family protein traR
ISU86_RS22435 (ISU86_22420) 18772..19266 + 495 WP_004187480 hypothetical protein -
ISU86_RS22440 (ISU86_22425) 19263..22313 + 3051 WP_004187482 hypothetical protein traU
ISU86_RS22445 (ISU86_22430) 22310..23518 + 1209 WP_011091082 conjugal transfer protein TraW traW
ISU86_RS22450 (ISU86_22435) 23515..24111 + 597 WP_015060003 hypothetical protein -
ISU86_RS22455 (ISU86_22440) 24104..26299 + 2196 WP_015062834 DotA/TraY family protein traY
ISU86_RS22460 (ISU86_22445) 26301..26954 + 654 WP_015060005 hypothetical protein -
ISU86_RS22465 (ISU86_22450) 27035..27265 + 231 WP_011091085 IncL/M type plasmid replication protein RepC -
ISU86_RS22470 (ISU86_22455) 27561..28616 + 1056 WP_015060006 plasmid replication initiator RepA -
ISU86_RS23415 29839..29934 + 96 WP_004206884 DinQ-like type I toxin DqlB -
ISU86_RS22475 (ISU86_22460) 29985..32072 - 2088 WP_004206885 conjugal transfer protein TrbC -
ISU86_RS22480 (ISU86_22465) 32085..33035 - 951 WP_004206886 DsbC family protein trbB
ISU86_RS22485 (ISU86_22470) 33046..34353 - 1308 WP_015059988 hypothetical protein trbA
ISU86_RS22490 (ISU86_22475) 34353..34748 - 396 WP_004187436 lytic transglycosylase domain-containing protein -
ISU86_RS22495 (ISU86_22480) 34853..35236 - 384 WP_019725042 DUF1496 domain-containing protein -
ISU86_RS22500 (ISU86_22485) 35316..35750 + 435 Protein_37 CPBP family intramembrane glutamate endopeptidase -
ISU86_RS22505 (ISU86_22490) 35765..36973 - 1209 WP_001206290 IS4-like element IS10A family transposase -
ISU86_RS22510 (ISU86_22495) 37276..38187 + 912 WP_015586033 LysR family transcriptional regulator -
ISU86_RS22515 (ISU86_22500) 38489..39286 - 798 WP_015059991 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-48 -

Host bacterium

ID   6125 GenBank   NZ_CP064115
Plasmid name   IR5283|unnamed2 Incompatibility group   IncL/M
Plasmid size   72217 bp Coordinate of oriT [Strand]   4109..4214 [+]
Host baterium   Enterobacter hormaechei strain IR5283

Cargo genes

Drug resistance gene   blaOXA-48, blaCTX-M-14b, aph(3'')-Ib, aph(3')-VIb, aph(6)-Id
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -