Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105687 |
| Name | oriT_pBD-50-Eh_VIM-1  |
| Organism | Enterobacter hormaechei subsp. steigerwaltii strain BD-50-Eh |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP063227 (11384..11473 [-], 90 nt) |
| oriT length | 90 nt |
| IRs (inverted repeats) | 34..41, 44..51 (AGTGCGAT..ATCGCACT) |
| Location of nic site | 62..63 |
| Conserved sequence flanking the nic site |
GGTGTATAGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 90 nt
>oriT_pBD-50-Eh_VIM-1
TATTTCATTTTTTGTTTTTTTAATCAGTTAGTTAGTGCGATTCATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC
TATTTCATTTTTTGTTTTTTTAATCAGTTAGTTAGTGCGATTCATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3847 | GenBank | WP_195207210 |
| Name | mobF_IE977_RS25455_pBD-50-Eh_VIM-1 |
UniProt ID | _ |
| Length | 1072 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1072 a.a. Molecular weight: 118954.63 Da Isoelectric Point: 6.4033
>WP_195207210.1 MobF family relaxase [Enterobacter hormaechei]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNDQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVENGDRFTVTGTTEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKKRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHSDGQQEKSYGHDAGHQTEEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDHDKEGYGL
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNDQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVENGDRFTVTGTTEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKKRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHSDGQQEKSYGHDAGHQTEEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDHDKEGYGL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 3854 | GenBank | WP_000139145 |
| Name | t4cp2_IE977_RS25450_pBD-50-Eh_VIM-1 |
UniProt ID | _ |
| Length | 510 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 510 a.a. Molecular weight: 57854.92 Da Isoelectric Point: 9.5852
>WP_000139145.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVAIIAGAVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAEKTKERGHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGTDKRIVLDPNGEYVETFYRPGDVILSPYDKRSPGWSMFNEIRDDFDYDLYAVSIIQ
ESKERSAEEWYRYGRLIYREVSKKLLTLKRSPTMEEVMHWCTIVENEKLKEFVKGTPAEALFIKGAERAT
GSARFVLGDKLAPHLKMPAGDFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTYSQLIERYGINIAETLLGSMRSGIVMGSSRL
GKETLEQMSRALGEIEGEVEHKQGNPFQAGIGNRPRIETRTVRAVTPTEISELKNLTGYVYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVAIIAGAVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAEKTKERGHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGTDKRIVLDPNGEYVETFYRPGDVILSPYDKRSPGWSMFNEIRDDFDYDLYAVSIIQ
ESKERSAEEWYRYGRLIYREVSKKLLTLKRSPTMEEVMHWCTIVENEKLKEFVKGTPAEALFIKGAERAT
GSARFVLGDKLAPHLKMPAGDFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTYSQLIERYGINIAETLLGSMRSGIVMGSSRL
GKETLEQMSRALGEIEGEVEHKQGNPFQAGIGNRPRIETRTVRAVTPTEISELKNLTGYVYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 51643..61931
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IE977_RS25645 (IE977_25645) | 47688..48095 | - | 408 | WP_101972618 | hypothetical protein | - |
| IE977_RS25650 (IE977_25650) | 48167..48685 | - | 519 | WP_244661197 | hypothetical protein | - |
| IE977_RS25655 (IE977_25655) | 48693..49142 | - | 450 | WP_101972616 | hypothetical protein | - |
| IE977_RS25660 (IE977_25660) | 49153..49692 | - | 540 | WP_101972615 | hypothetical protein | - |
| IE977_RS25670 (IE977_25670) | 50811..51122 | - | 312 | WP_000570332 | hypothetical protein | - |
| IE977_RS25675 (IE977_25675) | 51119..51631 | - | 513 | WP_029697418 | phospholipase D family protein | - |
| IE977_RS25680 (IE977_25680) | 51643..52644 | - | 1002 | WP_001076634 | ATPase, T2SS/T4P/T4SS family | virB11 |
| IE977_RS25685 (IE977_25685) | 52695..53828 | - | 1134 | WP_000101920 | type IV secretion system protein VirB10 | virB10 |
| IE977_RS25690 (IE977_25690) | 53828..54715 | - | 888 | WP_000758230 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| IE977_RS25695 (IE977_25695) | 54726..55421 | - | 696 | WP_001208352 | type IV secretion system protein | virB8 |
| IE977_RS26095 | 55432..55563 | - | 132 | WP_032493177 | hypothetical protein | - |
| IE977_RS25700 (IE977_25700) | 55640..56677 | - | 1038 | WP_101972614 | type IV secretion system protein | virB6 |
| IE977_RS25705 (IE977_25705) | 56694..56936 | - | 243 | WP_000858958 | EexN family lipoprotein | - |
| IE977_RS25710 (IE977_25710) | 56946..57659 | - | 714 | WP_000644929 | type IV secretion system protein | virB5 |
| IE977_RS25715 (IE977_25715) | 57675..60248 | - | 2574 | WP_032493176 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| IE977_RS25720 (IE977_25720) | 60249..60566 | - | 318 | WP_000462629 | VirB3 family type IV secretion system protein | virB3 |
| IE977_RS25725 (IE977_25725) | 60606..60902 | - | 297 | WP_015060075 | TrbC/VirB2 family protein | virB2 |
| IE977_RS25730 (IE977_25730) | 61149..61931 | - | 783 | WP_001642193 | lytic transglycosylase domain-containing protein | virB1 |
| IE977_RS25735 (IE977_25735) | 62031..62348 | + | 318 | WP_000960955 | H-NS family nucleoid-associated regulatory protein | - |
| IE977_RS25740 (IE977_25740) | 62352..62702 | + | 351 | WP_000528713 | hypothetical protein | - |
| IE977_RS25745 (IE977_25745) | 62699..63037 | + | 339 | WP_000040982 | hypothetical protein | - |
| IE977_RS25750 (IE977_25750) | 63041..63349 | + | 309 | WP_000733764 | hypothetical protein | - |
| IE977_RS25755 (IE977_25755) | 63634..63819 | + | 186 | WP_024562121 | ribbon-helix-helix domain-containing protein | - |
| IE977_RS25760 (IE977_25760) | 63816..64424 | - | 609 | WP_000275218 | recombinase family protein | - |
| IE977_RS25765 (IE977_25765) | 64590..64922 | - | 333 | WP_000140165 | hypothetical protein | - |
Host bacterium
| ID | 6124 | GenBank | NZ_CP063227 |
| Plasmid name | pBD-50-Eh_VIM-1 | Incompatibility group | IncN2 |
| Plasmid size | 65061 bp | Coordinate of oriT [Strand] | 11384..11473 [-] |
| Host baterium | Enterobacter hormaechei subsp. steigerwaltii strain BD-50-Eh |
Cargo genes
| Drug resistance gene | qnrB19, aph(3'')-Ib, aph(6)-Id, blaVIM-1, dfrB1, ant(3'')-Ia, aac(6')-Ib-cr, blaOXA-1, catB3, ARR-3, qacE, sul1, mph(A) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |