Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105682
Name   oriT_pEcl5-1 in_silico
Organism   Enterobacter hormaechei strain Eho-5
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP047737 (100470..100570 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_pEcl5-1
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3844 GenBank   WP_012561166
Name   mobF_GVI66_RS23845_pEcl5-1 insolico UniProt ID   D7RU03
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120141.30 Da        Isoelectric Point: 6.5229

>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB D7RU03


Auxiliary protein

ID   1744 GenBank   WP_001749975
Name   WP_001749975_pEcl5-1 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   3850 GenBank   WP_000342688
Name   t4cp2_GVI66_RS23850_pEcl5-1 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2429..11636

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI66_RS23390 (GVI66_23380) 1..876 + 876 WP_000594611 RepB family plasmid replication initiator protein -
GVI66_RS23395 (GVI66_23385) 2025..2378 + 354 WP_000423602 hypothetical protein -
GVI66_RS23400 (GVI66_23390) 2396..2746 + 351 WP_001447770 type IV conjugative transfer system protein TraL -
GVI66_RS23405 (GVI66_23395) 2758..3546 + 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
GVI66_RS23410 (GVI66_23400) 3546..4817 + 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
GVI66_RS23415 (GVI66_23405) 4819..5280 + 462 WP_021242895 plasmid transfer protein HtdO -
GVI66_RS23420 (GVI66_23410) 5270..6625 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
GVI66_RS23425 (GVI66_23415) 6633..7115 + 483 WP_000377632 hypothetical protein -
GVI66_RS23430 (GVI66_23420) 7234..7986 + 753 WP_182911723 protein-disulfide isomerase HtdT -
GVI66_RS23435 (GVI66_23425) 7996..8946 + 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
GVI66_RS23440 (GVI66_23430) 8955..11636 + 2682 WP_000387412 TraC family protein virb4
GVI66_RS23445 14185..14346 + 162 WP_001371949 hypothetical protein -

Region 2: 125941..136266

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI66_RS23985 (GVI66_23970) 121786..123219 - 1434 WP_001288432 DNA cytosine methyltransferase -
GVI66_RS23990 (GVI66_23975) 123318..123590 + 273 Protein_122 IS1 family transposase -
GVI66_RS23995 (GVI66_23980) 123601..123807 - 207 WP_001749967 hypothetical protein -
GVI66_RS24000 (GVI66_23985) 123812..124459 - 648 WP_015344958 restriction endonuclease -
GVI66_RS24005 (GVI66_23990) 124509..124820 - 312 WP_001452736 hypothetical protein -
GVI66_RS24010 (GVI66_23995) 124856..125170 - 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
GVI66_RS24015 (GVI66_24000) 125167..125511 - 345 WP_001749964 hypothetical protein -
GVI66_RS24020 (GVI66_24005) 125527..125832 - 306 WP_000960954 H-NS family nucleoid-associated regulatory protein -
GVI66_RS24025 (GVI66_24010) 125941..126675 + 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
GVI66_RS24030 (GVI66_24015) 126684..126965 + 282 WP_000440698 transcriptional repressor KorA -
GVI66_RS24035 (GVI66_24020) 126975..127268 + 294 WP_001749962 hypothetical protein virB2
GVI66_RS24040 (GVI66_24025) 127318..127635 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
GVI66_RS24045 (GVI66_24030) 127767..130235 + 2469 WP_224743086 VirB4 family type IV secretion/conjugal transfer ATPase virb4
GVI66_RS24050 (GVI66_24035) 130253..130966 + 714 WP_001749960 type IV secretion system protein virB5
GVI66_RS24055 (GVI66_24040) 130974..131201 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
GVI66_RS24060 (GVI66_24045) 131217..132257 + 1041 WP_001749958 type IV secretion system protein virB6
GVI66_RS25470 132358..132486 + 129 WP_071882546 conjugal transfer protein TraN -
GVI66_RS24065 (GVI66_24050) 132476..133174 + 699 WP_000646594 type IV secretion system protein virB8
GVI66_RS24070 (GVI66_24055) 133185..134069 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
GVI66_RS24075 (GVI66_24060) 134069..135229 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
GVI66_RS24080 (GVI66_24065) 135271..136266 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
GVI66_RS24085 (GVI66_24070) 136266..136799 + 534 WP_000792636 phospholipase D family protein -
GVI66_RS24090 (GVI66_24075) 136858..138249 - 1392 WP_014839878 ISKra4-like element ISKpn19 family transposase -
GVI66_RS24095 (GVI66_24080) 138286..138858 - 573 WP_001493762 recombinase family protein -
GVI66_RS24100 (GVI66_24085) 138995..139540 - 546 WP_001493763 plasmid pRiA4b ORF-3 family protein -
GVI66_RS24105 (GVI66_24090) 139676..139858 - 183 Protein_146 DUF4158 domain-containing protein -
GVI66_RS24110 (GVI66_24095) 140020..140634 + 615 WP_014839983 recombinase family protein -

Host bacterium

ID   6119 GenBank   NZ_CP047737
Plasmid name   pEcl5-1 Incompatibility group   IncHI2A
Plasmid size   310486 bp Coordinate of oriT [Strand]   100470..100570 [+]
Host baterium   Enterobacter hormaechei strain Eho-5

Cargo genes

Drug resistance gene   blaACC-1, dfrA14, qnrS1, sul1, aph(6)-Id, aph(3'')-Ib, mcr-9, qacE, aadA2, dfrA12, aac(3)-IId, blaTEM-1B, catA1
Virulence gene   -
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE, silE, silS, silR, silC, silF, silB, silA, silP, pcoS, pcoE, ncrC, rcnR/yohL, arsC, arsB, arsR, arsH, merR, merT, merP, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -