Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105667
Name   oriT_pLG791-optrA in_silico
Organism   Lactococcus garvieae strain LG791
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP071291 (44751..44772 [-], 22 nt)
oriT length   22 nt
IRs (inverted repeats)      2..8, 13..19  (ACTTTAT..ATAAAGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 22 nt

>oriT_pLG791-optrA
CACTTTATGAATATAAAGTATA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3837 GenBank   WP_206886671
Name   Relaxase_J0J34_RS09760_pLG791-optrA insolico UniProt ID   _
Length   511 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 511 a.a.        Molecular weight: 59573.48 Da        Isoelectric Point: 9.3810

>WP_206886671.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactococcus]
MVVVPKPKQIKSEKALKSAVKYILNPQKTDEQVLTSGFHINNLNFGDVEMGYTRMLARKMVGRQNKQVLA
QHLVQSFRYEDGLSAEEIHQIGREWIEQLTGGKHEYIIATHIDKGHIHNHIIFNTTSNVDFKNFRWKKDT
LKIARELSDKVSLKHGAILENTSPYQKSYHSYQKYLAENPIRPELKSRLNFLLKHSLSLDDFKIKAAALN
VTVDFSGKYTTYRLNDYEQKRPIRDSSLISKKDQSSMKTNPEKRIFSKEMIEKKCIQNQDMKKKTLSSSE
ILAEYQKQQSWYKENSNIKLVLEDWQVDQEVDSGIYIFVQAGHREGSIKIPHSAIDRNEDGKLEIHISSY
SKFAFMDAKNPSESYVLWGHQVISQLSRENDNVPIYNNKAMNNVHNLFDAMNLLAKHGVTGRESFEHLGE
DFVSEMREIEASLDKLDKLIQDKTDSIKFNQHDNVQVQQLKNLQEERKDLQKSYNKITADLELYDNIQNF
NQVKDKQRKQEERKEQPHAKL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(13-246)


  Protein structure



No available structure.




T4CP

ID   3844 GenBank   WP_206886660
Name   t4cp2_J0J34_RS09690_pLG791-optrA insolico UniProt ID   _
Length   600 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 600 a.a.        Molecular weight: 68438.06 Da        Isoelectric Point: 6.4023

>WP_206886660.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Lactococcus]
MKKVVNKLPLTITTLLLMWLANRTTQIYIDTPGTFATTKVTTFQENLPYELLKNPFQFSLNSFPLYATAF
IGAVVLLFISLKDTRKYRKDEEFGSGREGTEEDLKSFANKEFSQNIILAEQGLMSITNDYKDPVLQRNKN
VMVVGGPGSWKTTAYNIPNVSQMNASYIVTDPKGEIRNMTGKMMERHGYKVKVLDLKTLTNTDQFNPFAY
TKSEEILQDVITQIIAAGNADMQKNGEPFWDNAEQLLLSSLFMYLYYNYRGYKGKEGSGELPCLADVADL
LRNLERQDPDVKSPVELMFDDFEEYFGSDNPAVLNFQSFKNFSGETRSSVVSIPTARFRMFNLESVQNLT
RRDTLELEKLGTEKTIIYVVLSDLNTTYNLFANLLFMLSFQVLEDVADYKHGGRLPLPVRMIMEEFPSIG
RIPNILQAIAIFRGRGVGFEFTCQNFDQLKRIYKDTWEEIVGSCETVVYMSGATTKLTTTTMSERARKET
IAIKDTSDSRGKSGSSSRSYRRMGRDVFMPDEVESLGRLYALVKISGDVPVVKLKKYNSKKHPRAKEWGK
KPDDENWYHYTRYKDKLDELEHSIAESSNSELVVTTLDVA

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(139-546)

  Protein structure



No available structure.




Host bacterium

ID   6104 GenBank   NZ_CP071291
Plasmid name   pLG791-optrA Incompatibility group   -
Plasmid size   76771 bp Coordinate of oriT [Strand]   44751..44772 [-]
Host baterium   Lactococcus garvieae strain LG791

Cargo genes

Drug resistance gene   aac(6')-aph(2''), erm(B), tet(L), tet(O/W/32/O), fexA, optrA
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -