Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105652
Name   oriT_pMS-37b in_silico
Organism   Enterobacter hormaechei strain EGYMCRVIM
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP053192 (47530..47680 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pMS-37b
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3834 GenBank   WP_001326173
Name   mobH_HK406_RS24240_pMS-37b insolico UniProt ID   F5BPR1
Length   990 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB F5BPR1


T4CP

ID   3841 GenBank   WP_000178857
Name   traD_HK406_RS24245_pMS-37b insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69225.71 Da        Isoelectric Point: 6.9403

>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-299)

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

  Protein structure



No available structure.



ID   3842 GenBank   WP_000637384
Name   traC_HK406_RS24325_pMS-37b insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92604.86 Da        Isoelectric Point: 5.9216

>WP_000637384.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRRQAA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

TraG P-loop domain (P-loop_TraG)

(449-809)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 45614..70760

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HK406_RS24230 41645..41878 - 234 WP_001191890 hypothetical protein -
HK406_RS24235 41860..42477 - 618 WP_001249395 hypothetical protein -
HK406_RS24240 42645..45617 + 2973 WP_001326173 MobH family relaxase -
HK406_RS24245 45614..47479 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
HK406_RS24250 47529..48074 + 546 WP_000228720 hypothetical protein -
HK406_RS24255 48031..48660 + 630 WP_000743449 DUF4400 domain-containing protein tfc7
HK406_RS24260 48670..49116 + 447 WP_000122507 hypothetical protein -
HK406_RS24265 49126..49503 + 378 WP_000869297 hypothetical protein -
HK406_RS24270 49503..50165 + 663 WP_001231464 hypothetical protein -
HK406_RS24275 50346..50489 + 144 WP_001275801 hypothetical protein -
HK406_RS24280 50501..50866 + 366 WP_001052531 hypothetical protein -
HK406_RS24285 51011..51292 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
HK406_RS24290 51289..51915 + 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
HK406_RS24295 51899..52816 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
HK406_RS24300 52816..54129 + 1314 WP_024131605 TraB/VirB10 family protein traB
HK406_RS24305 54126..54704 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
HK406_RS24310 54708..55100 + 393 WP_000479535 TraA family conjugative transfer protein -
HK406_RS24315 55301..60787 + 5487 WP_170851375 Ig-like domain-containing protein -
HK406_RS24320 60936..61643 + 708 WP_001259346 DsbC family protein -
HK406_RS24325 61640..64087 + 2448 WP_000637384 type IV secretion system protein TraC virb4
HK406_RS24330 64102..64419 + 318 WP_000351984 hypothetical protein -
HK406_RS24335 64416..64946 + 531 WP_001010740 S26 family signal peptidase -
HK406_RS24340 64909..66174 + 1266 WP_001447719 TrbC family F-type conjugative pilus assembly protein traW
HK406_RS24345 66171..66842 + 672 WP_170851376 EAL domain-containing protein -
HK406_RS24350 66842..67846 + 1005 WP_005507569 TraU family protein traU
HK406_RS24355 67962..70760 + 2799 WP_024126922 conjugal transfer mating pair stabilization protein TraN traN
HK406_RS24360 70799..71659 - 861 WP_000709517 hypothetical protein -
HK406_RS24365 71782..72423 - 642 WP_000796664 hypothetical protein -
HK406_RS24370 72717..73037 + 321 WP_000547566 hypothetical protein -
HK406_RS24375 73341..73526 + 186 WP_001186917 hypothetical protein -
HK406_RS24380 73746..74714 + 969 WP_000085162 AAA family ATPase -
HK406_RS24385 74725..75633 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   6089 GenBank   NZ_CP053192
Plasmid name   pMS-37b Incompatibility group   IncA/C2
Plasmid size   129016 bp Coordinate of oriT [Strand]   47530..47680 [+]
Host baterium   Enterobacter hormaechei strain EGYMCRVIM

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -