Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105650
Name   oriT_AKB48|unnamed2 in_silico
Organism   Enterobacter hormaechei strain AKB48
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP044337 (1092..1197 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_AKB48|unnamed2
AGTACGGGACAAGATGTGTTTTTGGGGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3832 GenBank   WP_169724910
Name   Relaxase_D6T76_RS22670_AKB48|unnamed2 insolico UniProt ID   _
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75189.93 Da        Isoelectric Point: 9.9948

>WP_169724910.1 TraI/MobA(P) family conjugative relaxase [Enterobacter hormaechei]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNPVEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
VKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure



No available structure.




T4CP

ID   3839 GenBank   WP_169724938
Name   t4cp2_D6T76_RS22975_AKB48|unnamed2 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79546.86 Da        Isoelectric Point: 4.9068

>WP_169724938.1 conjugal transfer protein TrbC [Enterobacter hormaechei]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETRAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNPVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDARVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 3993..23093

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6T76_RS22655 (D6T76_22960) 1..319 + 319 Protein_0 hypothetical protein -
D6T76_RS22660 (D6T76_22965) 803..1012 - 210 WP_249327047 hypothetical protein -
D6T76_RS22665 (D6T76_22970) 1199..1516 + 318 WP_011091068 plasmid mobilization protein MobA -
D6T76_RS22670 (D6T76_22975) 1503..3482 + 1980 WP_169724910 TraI/MobA(P) family conjugative relaxase -
D6T76_RS22675 (D6T76_22980) 3496..3996 + 501 WP_169724911 DotD/TraH family lipoprotein -
D6T76_RS22680 (D6T76_22985) 3993..4772 + 780 WP_169724912 type IV secretory system conjugative DNA transfer family protein traI
D6T76_RS22685 (D6T76_22990) 4783..5946 + 1164 WP_169724913 plasmid transfer ATPase TraJ virB11
D6T76_RS22690 (D6T76_22995) 5936..6196 + 261 WP_004187310 IcmT/TraK family protein traK
D6T76_RS22695 (D6T76_23000) 6221..7582 + 1362 WP_169724914 toprim domain-containing protein -
D6T76_RS22700 (D6T76_23005) 7754..9430 + 1677 WP_169724915 LPD7 domain-containing protein -
D6T76_RS22705 (D6T76_23010) 9396..9908 + 513 WP_011091071 hypothetical protein traL
D6T76_RS22710 (D6T76_23015) 9909..10121 - 213 WP_305953721 Hha/YmoA family nucleoid-associated regulatory protein -
D6T76_RS22715 (D6T76_23020) 10093..10503 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
D6T76_RS22720 (D6T76_23025) 10571..11284 + 714 WP_249327049 DotI/IcmL family type IV secretion protein traM
D6T76_RS22725 (D6T76_23030) 11293..12444 + 1152 WP_169724917 DotH/IcmK family type IV secretion protein traN
D6T76_RS22730 (D6T76_23035) 12456..13805 + 1350 WP_169724918 conjugal transfer protein TraO traO
D6T76_RS22735 (D6T76_23040) 13817..14521 + 705 WP_169724919 conjugal transfer protein TraP traP
D6T76_RS22740 (D6T76_23045) 14545..15075 + 531 WP_169724920 conjugal transfer protein TraQ traQ
D6T76_RS22745 (D6T76_23050) 15092..15481 + 390 WP_169724921 DUF6750 family protein traR
D6T76_RS22750 (D6T76_23055) 15527..16021 + 495 WP_249327051 hypothetical protein -
D6T76_RS22755 (D6T76_23060) 16018..19068 + 3051 WP_169724922 conjugal transfer protein traU
D6T76_RS22760 (D6T76_23065) 19065..20273 + 1209 WP_169724923 conjugal transfer protein TraW traW
D6T76_RS22765 (D6T76_23070) 20270..20920 + 651 WP_011091083 hypothetical protein -
D6T76_RS22770 (D6T76_23075) 20913..23093 + 2181 WP_169724924 DotA/TraY family protein traY
D6T76_RS22775 (D6T76_23080) 23096..23749 + 654 WP_169724925 ExcA -
D6T76_RS22780 (D6T76_23085) 23824..24054 + 231 WP_169724926 IncL/M type plasmid replication protein RepC -
D6T76_RS22785 (D6T76_23090) 24352..25407 + 1056 WP_249327053 plasmid replication initiator RepA -
D6T76_RS22790 (D6T76_23095) 26411..26938 - 528 Protein_27 class A beta-lactamase -
D6T76_RS22795 (D6T76_23100) 26972..27676 - 705 WP_151316232 IS6-like element IS26 family transposase -

Host bacterium

ID   6087 GenBank   NZ_CP044337
Plasmid name   AKB48|unnamed2 Incompatibility group   IncL/M
Plasmid size   94408 bp Coordinate of oriT [Strand]   1092..1197 [+]
Host baterium   Enterobacter hormaechei strain AKB48

Cargo genes

Drug resistance gene   aac(3)-IId, dfrA12, aadA2, qacE, sul1, armA, msr(E), mph(E), blaCTX-M-3
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -