Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105645
Name   oriT_pIHI2-233 in_silico
Organism   Enterobacter hormaechei strain Y233
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP049047 (2300..2554 [+], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_pIHI2-233
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3829 GenBank   WP_242697246
Name   mobH_G5B34_RS22450_pIHI2-233 insolico UniProt ID   _
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118095.52 Da        Isoelectric Point: 4.6347

>WP_242697246.1 MobH family relaxase [Enterobacter hormaechei]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPVLPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure



No available structure.




T4CP

ID   3837 GenBank   WP_001284073
Name   traD_G5B34_RS24235_pIHI2-233 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 181371..190578

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5B34_RS23425 178661..178822 - 162 WP_001371949 hypothetical protein -
G5B34_RS23430 (G5B34_23360) 181371..184052 - 2682 WP_000387412 TraC family protein virb4
G5B34_RS23435 (G5B34_23365) 184061..185011 - 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
G5B34_RS23440 (G5B34_23370) 185021..185773 - 753 WP_182911723 protein-disulfide isomerase HtdT -
G5B34_RS23445 (G5B34_23375) 185892..186374 - 483 WP_000377632 hypothetical protein -
G5B34_RS23450 (G5B34_23380) 186382..187737 - 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
G5B34_RS23455 (G5B34_23385) 187727..188188 - 462 WP_000521242 plasmid transfer protein HtdO -
G5B34_RS23460 (G5B34_23390) 188190..189461 - 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
G5B34_RS23465 (G5B34_23395) 189461..190249 - 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
G5B34_RS23470 (G5B34_23400) 190261..190611 - 351 WP_001447770 type IV conjugative transfer system protein TraL -
G5B34_RS23475 (G5B34_23405) 190629..190982 - 354 WP_000423602 hypothetical protein -
G5B34_RS23480 (G5B34_23410) 191441..192388 + 948 WP_031285326 IS30 family transposase -
G5B34_RS23485 (G5B34_23415) 193221..194096 - 876 WP_000594611 RepB family plasmid replication initiator protein -
G5B34_RS23490 (G5B34_23420) 194573..195340 + 768 WP_242697243 hypothetical protein -

Host bacterium

ID   6082 GenBank   NZ_CP049047
Plasmid name   pIHI2-233 Incompatibility group   IncHI2A
Plasmid size   322325 bp Coordinate of oriT [Strand]   2300..2554 [+]
Host baterium   Enterobacter hormaechei strain Y233

Cargo genes

Drug resistance gene   aph(3')-Ia, mcr-9, aph(6)-Id, aph(3'')-Ib, dfrA19, blaTEM-1B, aac(6')-IIc, ere(A), qacE, sul1, qnrB4, blaDHA-1, blaSHV-12, catA2, tet(D)
Virulence gene   -
Metal resistance gene   arsH, arsR, arsB, arsC, rcnR/yohL, ncrC, pcoE, pcoS, merR, merT, merP, merC, merA, merD, merE, pbrA, terE, terD, terC, terB, terA, terZ, terW
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -