Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105632
Name   oriT_S11_16|unnamed2 in_silico
Organism   Enterobacter hormaechei strain S11_16
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP035387 (50028..50127 [+], 100 nt)
oriT length   100 nt
IRs (inverted repeats)      77..82, 90..95  (AAAAAA..TTTTTT)
 78..83, 90..95  (AAAAAA..TTTTTT)
 79..84, 90..95  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 100 nt

>oriT_S11_16|unnamed2
TATTTATTTTTTTATCTTTTAAATCAGTACGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGAAAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3823 GenBank   WP_012561142
Name   mobF_EQ802_RS24660_S11_16|unnamed2 insolico UniProt ID   A0A1S6KKJ9
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120195.37 Da        Isoelectric Point: 6.5230

>WP_012561142.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKADMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

AAA domain (AAA_30)

(477-653)

TrwC relaxase (TrwC)

(16-287)


  Protein structure


Source ID Structure
AlphaFold DB A0A1S6KKJ9


Auxiliary protein

ID   1738 GenBank   WP_001749975
Name   WP_001749975_S11_16|unnamed2 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   3832 GenBank   WP_000342688
Name   t4cp2_EQ802_RS24665_S11_16|unnamed2 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 12960..23289

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQ802_RS24420 (EQ802_24425) 8226..8831 - 606 WP_000509966 recombinase family protein -
EQ802_RS24425 (EQ802_24430) 8926..11823 + 2898 WP_087588792 Tn3-like element Tn5403 family transposase -
EQ802_RS24430 (EQ802_24435) 11857..12504 - 648 Protein_9 IS1182-like element ISKpn6 family transposase -
EQ802_RS25025 12856..12960 - 105 Protein_10 phospholipase D family protein -
EQ802_RS24440 (EQ802_24445) 12960..13955 - 996 WP_012561144 ATPase, T2SS/T4P/T4SS family virB11
EQ802_RS24445 (EQ802_24450) 13997..15157 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
EQ802_RS24450 (EQ802_24455) 15157..16041 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
EQ802_RS24455 (EQ802_24460) 16052..16750 - 699 WP_000646594 type IV secretion system protein virB8
EQ802_RS25315 (EQ802_24465) 16740..16898 - 159 WP_012561180 hypothetical protein -
EQ802_RS24465 (EQ802_24470) 16969..18009 - 1041 WP_001749958 type IV secretion system protein virB6
EQ802_RS24470 (EQ802_24475) 18025..18252 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
EQ802_RS24475 (EQ802_24480) 18260..18973 - 714 WP_001749960 type IV secretion system protein virB5
EQ802_RS24480 (EQ802_24485) 18991..21459 - 2469 WP_223669807 VirB4 family type IV secretion/conjugal transfer ATPase virb4
EQ802_RS24485 (EQ802_24490) 21591..21908 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
EQ802_RS24490 (EQ802_24495) 21959..22252 - 294 WP_001749962 hypothetical protein virB2
EQ802_RS24495 (EQ802_24500) 22262..22543 - 282 WP_016338364 transcriptional repressor KorA -
EQ802_RS24500 (EQ802_24505) 22552..23289 - 738 WP_013279384 lytic transglycosylase domain-containing protein virB1
EQ802_RS24505 (EQ802_24510) 23332..23703 + 372 WP_223174968 H-NS family nucleoid-associated regulatory protein -
EQ802_RS24510 (EQ802_24515) 23719..24063 + 345 WP_012561155 hypothetical protein -
EQ802_RS24515 (EQ802_24520) 24060..24374 + 315 WP_016338363 TrbM/KikA/MpfK family conjugal transfer protein -
EQ802_RS24520 (EQ802_24525) 24488..24721 + 234 WP_012561157 hypothetical protein -
EQ802_RS25245 24777..25037 + 261 WP_016359294 hypothetical protein -
EQ802_RS24530 (EQ802_24535) 25079..26047 - 969 WP_016151349 IS5 family transposase -
EQ802_RS25275 26065..26196 - 132 WP_255255389 hypothetical protein -
EQ802_RS24535 (EQ802_24540) 26131..26484 + 354 WP_225622145 restriction endonuclease -
EQ802_RS24540 (EQ802_24545) 26489..26695 + 207 WP_001749967 hypothetical protein -
EQ802_RS24545 (EQ802_24550) 26706..26978 - 273 Protein_33 IS1 family transposase -

Host bacterium

ID   6069 GenBank   NZ_CP035387
Plasmid name   S11_16|unnamed2 Incompatibility group   IncN
Plasmid size   63369 bp Coordinate of oriT [Strand]   50028..50127 [+]
Host baterium   Enterobacter hormaechei strain S11_16

Cargo genes

Drug resistance gene   blaTEM-1B, blaKPC-2, qnrB5, sul1, dfrA19, aph(3'')-Ib, aph(6)-Id
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -