Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105631
Name   oriT_S11_16|unnamed1 in_silico
Organism   Enterobacter hormaechei strain S11_16
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP035386 (93383..93485 [-], 103 nt)
oriT length   103 nt
IRs (inverted repeats)      89..94, 96..101  (AAATAA..TTATTT)
 28..33, 40..45  (GCAAAA..TTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 103 nt

>oriT_S11_16|unnamed1
CTCACCACCAAAAGCTCCACACCCCACGCAAAATTAAAGTTTTGCTGATTGGGTATTCAAATCATGCAGTTAGTGAAAAAGTAATGTGAAATAATTTATTTTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3822 GenBank   WP_023307204
Name   traI_EQ802_RS24045_S11_16|unnamed1 insolico UniProt ID   A0A1Q1PS85
Length   1746 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1746 a.a.        Molecular weight: 189264.44 Da        Isoelectric Point: 6.1121

>WP_023307204.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMGERWAGKGAEALGLSGGVDQKVFTKVLEGRLPDGSDLSRTQ
DGANKHRPGYDFTFSAPKSVSVMAMLGGDKRLIEAHNRAVETAIKQIEAMASTRVMTEGRSETQLTGNLV
MALFNHDTSRDQEPQLHTHAVVANATQHGDEWRTLSSDKVGKTGFIENIYANQIAFGRLYRAALKDDVAA
MGYETETVGKHGMWELKGVPTEPYSSRSRTISEAVGDDASLKSRDVAALDTRQSKQKVDPEQRMAEWMHT
LKETGFDIKAYREAADLRVVQGNIPATTPEAIDINSSVGQAIAMLSDRRARFTYSELLATTLGQLPARSG
MVEMARDGIDAAIKNEQLIPLDKEKGLFTSNIHVLDELSVSAMTRDLQRTGQADIFPDKSVPRSRSYSDA
VSVLAQDRPPVAIISGQGGAAGQRERVAELTMMAREQGRDVQIIAADRRSRSALMQDERLSGEHITDRRG
LTEGMAFTPGSTLIVDQGEKLTLKETLTLLDGALRHNVQLLIADSGQRTGTGSALTVMKEAGVSTLAWQG
GEKTQVSVISEPDRRQRYDRLAADFARSVRAGEHSVAQVTGPREQAVLAGVVREALKAEKVLGEREVSIT
TLEPVWLDSRHQQVRDHYREGMVMERWNAEERSRERFVIDRVTGRNNSLTLRNAQGETRVTRLSELNSSW
SLYRTGTLQVAEGDRLAVLGQAQGTRLRGGDTVTVKALGEAGLVVSLPGRKADVVLPAGDSPFTAAKVVQ
GWVESPGRSVSDSATVFASLSQRELDNTTLNKLALSGRAVRLYSAQTAQKTTEKLSRQSAWSVVSEQIKD
AAGHDRLDDALAHQKAGLHTPEQQAIHLAIPVLEGNGLAFTKPQLMAAAKEFEQDSLSLQAIEKEADRQV
RSGDLLSVPVSPGNGLQLLVSRQSYNAEKSIIRHVLEGKEAVTPLMDKVPDAQLAGLTDGQRNATRMILE
SPDRFTLVQGYAGVGKTTQFRAVMSAIGTLPEREQPRVIGVAPTHRAVSEMRDAGVPETQTLAAFIHDTQ
QQLRGGERTDFSNVLFLVDESSMVGNADMAKAYSLIAGGGGRAVSSGDTDQLQSIAPGQPFRLLQKRSAI
DVAVMQEIVRQTPELKPAVYSLISRDIGSALTTIESVAPAQVPRRADAWQPDSSVMEFSREREEAIAKAV
AAGDLMPGGQPATLLEAIVKDYTGRTSEAQAQTIVITALNADRRQVNAMIHDARQGAGEVGEKEVTLPVL
TPANIRDGELRRMATWEASRDSLVLLDSTYYSIEALDGEAHLVMLKDAQGNTRSLSPAQAATEGVTLYRQ
DTITVSEGDRMRFSKSDNERGFVANSVWSVSEIKGDSVTLSDGKQTRTLTPSAEQAEQHIDLAYAVTVEG
SQGASEPFSISLQGTDGGRKRMVSFESAYVALSRMKQHAQVYTDNRDKWVAAMEKSQAKSTAHDILEPRG
DRAVANAARLTATAKALGEVPAGRAALRQAGLQPEGSMAKYISPGRKYPQPHVALPAFDRNGRKAGVWLS
ALTSGDGQLKGLAGEGRVMGSGDAAFAGLQASRNGESLLARDMEEGVRLARENPQSGVVVRLEGEARPWN
PGAITGGRVWADGLPDATGTQPAETVPPEVLAQQAREAEVQRELDKRAEEAVREMARSGDRPAGGADVAV
RDVVRDMDRIKETPASPLTLPDAPEERRRDEAVSQVVRESVQRDRLQQMERETVRDLEREKTLGGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(631-710)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(573-625)

AAA domain (AAA_30)

(967-1150)

TrwC relaxase (TrwC)

(10-283)

DNA helicase TraI (TraI_C)

(1430-1551)


  Protein structure



No available structure.




T4CP

ID   3830 GenBank   WP_023307203
Name   traD_EQ802_RS24050_S11_16|unnamed1 insolico UniProt ID   _
Length   733 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 733 a.a.        Molecular weight: 82212.80 Da        Isoelectric Point: 5.1609

>WP_023307203.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIASMRIRMFSQIANIIVYCLVILFGIIVAITLSVKLTWQTFVNGMVYYWCQTLAPMR
DIFKSQPVYTIRYYGHELKYNAQQILQDKYIQWCADQVWTAFILAAIIAAVVCVITFFVTTWILGHQGKQ
QSEDEITGGRQLTDDPKAVAKMLRRDGLASDIKIGELPLIKDSEIQNFCLHGTVGAGKSEVIRRLMNYAR
QRGDMVIVYDRSCEFVKSYYDPATDKILNPLDARCAAWDLWRECLTQPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDKDRSYSKLVDTLLSIKIEKLRSYLKNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIENNGESFTIRDWMRGVREDGKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVIGIQSYAQLEDIYGQKPAETLFDVLNTRAFFRSPSRNIA
EFAAGEIGEKEILKASEQYSYGVDPVRDGTSVGKDMERVTLVSYSDVQTLPDLSCYVTLPGPYPAVKMAL
KYQQRPKVAPEFIPRTIDGKAEERLNAALAAREHESRSIAMLFEPDADVVAPEGGAENVGQPQQPQQPQQ
PQQPQQPQQPQQLTPAPRAADAASVAGAAGAGGVEPELKTKAEEAEQLPPGISESGEVVDMAAWEAWQAE
QDELSPQERQRREEVNINVHRAPEKDLEPGGYI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.



ID   3831 GenBank   WP_008324134
Name   traC_EQ802_RS24125_S11_16|unnamed1 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 98841.51 Da        Isoelectric Point: 5.7676

>WP_008324134.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacterales]
MSNPLDSVTRTVNSLLTALKMPDASSEANDVLGQMRFPQFSRVLPYRDYDAETGLFMNEKTMGFMLEAQP
INGANETIVSSLENLMRTKLPREVPVAVHLVSSHLVGQDIEYGLRELSWTGEKADTFNAITRAYYLRAAE
EGFPLPDHLNLPLTLRNYRVYISCCVPRKRGSKASVMEMENQVKIIRASLQGAQIATRSVDADAFIRIAG
EMINHNPDALYPTERELDPLKDLNYQCVDDTFDLKVKADYMTLGLRGNGRNSTARIMNFQLTKNPELAFL
WNMADNYSNLLNPELSVSCPFILTLVLSVEDQVKAQNEANLKFMDLEKKSKTSYAKFFPGVAQEAKEWGD
LRQRLSSGQSSLVSYFLNVTLFCRDDQETALKVEQDVLNSFRKNGFELISPRFNHMRNFLVNLPFMAGGG
LFKQLRESGVLQRAESFNVANLMPVVADSPLATSGLLAPTYRNQLAFIDLFSRGMGNTNFNMAVCGTSGA
GKTGLIQPLIRSVIDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLKFNPFANVTDDTIDEMAERLRDQL
SVMASPNGNLDEVHEGLLLKAVKATWLTKKNQARIDDVVDYLMMARDSEEYSGSPTIRSRLDEMVVLLTQ
YTRDGVYGSYFNSDEPSLKDEARMVVLELGGLEDRPSLLVAVMFSLILYIENRMYRTPRSLKKLNVIDEG
WRLLDFKNNKVGAFIEKGYRTARRHTGSYITITQNIVDFDSETASSAARAAWGNSSYKIILKQSAKEFAK
YNQLYPDQYSQLQRDMIKGFGSAKDNWFSSFMLSVEHLTSWHRLFVDPLSRAMYSSDGPDFEFLQQRRRE
GLSIHEAVWELAWKKSGPEMAALERWLHEHEGKTIS

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(289-446)

TraG P-loop domain (P-loop_TraG)

(470-765)

TraC protein (TraC_F_IV)

(38-276)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 68908..94033

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQ802_RS24050 (EQ802_24055) 68908..71109 - 2202 WP_023307203 type IV conjugative transfer system coupling protein TraD virb4
EQ802_RS24055 (EQ802_24060) 71243..71917 - 675 WP_008324909 tetratricopeptide repeat protein -
EQ802_RS24060 (EQ802_24065) 71992..72423 - 432 WP_008324118 entry exclusion protein -
EQ802_RS24065 (EQ802_24070) 72445..75267 - 2823 WP_008324119 conjugal transfer mating-pair stabilization protein TraG traG
EQ802_RS24070 (EQ802_24075) 75267..76637 - 1371 WP_008324121 conjugal transfer pilus assembly protein TraH traH
EQ802_RS24075 (EQ802_24080) 76624..77052 - 429 WP_008324122 hypothetical protein -
EQ802_RS24080 (EQ802_24085) 77045..77599 - 555 WP_008324125 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
EQ802_RS24085 (EQ802_24090) 77589..77837 - 249 WP_008324126 type-F conjugative transfer system pilin chaperone TraQ -
EQ802_RS24090 (EQ802_24095) 77854..78603 - 750 WP_008324127 type-F conjugative transfer system pilin assembly protein TraF traF
EQ802_RS24095 (EQ802_24100) 78634..78822 - 189 WP_008324128 conjugal transfer protein TrbE -
EQ802_RS24100 (EQ802_24105) 78854..80707 - 1854 WP_022652255 type-F conjugative transfer system mating-pair stabilization protein TraN traN
EQ802_RS24105 (EQ802_24110) 80704..81333 - 630 WP_008324130 type-F conjugative transfer system pilin assembly protein TrbC trbC
EQ802_RS24110 (EQ802_24115) 81350..82336 - 987 WP_032160671 conjugal transfer pilus assembly protein TraU traU
EQ802_RS24115 (EQ802_24120) 82339..82986 - 648 WP_008324132 type-F conjugative transfer system protein TraW traW
EQ802_RS24120 (EQ802_24125) 82986..83333 - 348 WP_008324133 type-F conjugative transfer system protein TrbI -
EQ802_RS24125 (EQ802_24130) 83330..85960 - 2631 WP_008324134 type IV secretion system protein TraC virb4
EQ802_RS24130 (EQ802_24135) 85953..86519 - 567 WP_008324135 conjugal transfer pilus-stabilizing protein TraP -
EQ802_RS24135 (EQ802_24140) 86509..86910 - 402 WP_008324137 hypothetical protein -
EQ802_RS24140 (EQ802_24145) 86894..87079 - 186 WP_008324139 hypothetical protein -
EQ802_RS24145 (EQ802_24150) 87083..87307 - 225 WP_008324141 TraR/DksA C4-type zinc finger protein -
EQ802_RS24150 (EQ802_24155) 87427..87966 - 540 WP_008324154 type IV conjugative transfer system lipoprotein TraV traV
EQ802_RS24155 (EQ802_24160) 87988..89382 - 1395 WP_008324156 F-type conjugal transfer pilus assembly protein TraB traB
EQ802_RS24160 (EQ802_24165) 89369..90112 - 744 WP_008324157 type-F conjugative transfer system secretin TraK traK
EQ802_RS24165 (EQ802_24170) 90099..90665 - 567 WP_008324158 type IV conjugative transfer system protein TraE traE
EQ802_RS24170 (EQ802_24175) 90680..90985 - 306 WP_008324160 type IV conjugative transfer system protein TraL traL
EQ802_RS24175 (EQ802_24180) 91136..91486 - 351 WP_008324162 type IV conjugative transfer system pilin TraA -
EQ802_RS24180 (EQ802_24185) 91544..91768 - 225 WP_008324165 TraY domain-containing protein -
EQ802_RS24185 (EQ802_24190) 91855..92544 - 690 WP_008324166 hypothetical protein -
EQ802_RS24190 (EQ802_24195) 92733..93125 - 393 WP_008324167 conjugal transfer relaxosome DNA-binding protein TraM -
EQ802_RS24195 (EQ802_24200) 93557..94033 + 477 WP_008324168 transglycosylase SLT domain-containing protein virB1
EQ802_RS24200 (EQ802_24205) 94080..94610 - 531 WP_008324170 antirestriction protein -
EQ802_RS24205 (EQ802_24210) 95237..96067 - 831 WP_008324171 N-6 DNA methylase -
EQ802_RS24210 (EQ802_24215) 96110..96373 - 264 WP_008324174 hypothetical protein -
EQ802_RS24215 (EQ802_24220) 96459..96803 - 345 WP_008324177 hypothetical protein -
EQ802_RS24220 (EQ802_24225) 96850..97137 - 288 WP_008324178 hypothetical protein -
EQ802_RS24225 (EQ802_24230) 97191..97565 - 375 WP_008324180 hypothetical protein -
EQ802_RS24230 (EQ802_24235) 98192..98551 - 360 WP_008324181 hypothetical protein -
EQ802_RS24235 (EQ802_24240) 98613..98936 - 324 WP_008324183 hypothetical protein -

Host bacterium

ID   6068 GenBank   NZ_CP035386
Plasmid name   S11_16|unnamed1 Incompatibility group   IncFIB
Plasmid size   119994 bp Coordinate of oriT [Strand]   93383..93485 [-]
Host baterium   Enterobacter hormaechei strain S11_16

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -