Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105629
Name   oriT_pCTXM65_090011 in_silico
Organism   Enterobacter hormaechei strain WCHEH090011
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP036311 (42229..42323 [-], 95 nt)
oriT length   95 nt
IRs (inverted repeats)      73..78, 85..90  (AAAAAA..TTTTTT)
 73..78, 84..89  (AAAAAA..TTTTTT)
 27..34, 37..44  (AGCGTGAT..ATCACGCT)
 13..19, 31..37  (TAAATCA..TGATTTA)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 95 nt

>oriT_pCTXM65_090011
TTTTTTTTCTTTTAAATCAGTGAGATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3820 GenBank   WP_242637960
Name   mobF_EKN58_RS22885_pCTXM65_090011 insolico UniProt ID   _
Length   959 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 959 a.a.        Molecular weight: 107096.92 Da        Isoelectric Point: 6.8278

>WP_242637960.1 MobF family relaxase [Enterobacter hormaechei]
MPAEKLAQARTTRQGKSVTQNTNNLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRN
KMHLGDVYKQELALELTKAGYELRYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQT
KSRVSMATREKKTEHSREEIHQEWASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAI
QFAVKSLSERDASFERQKLIQIANKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAET
LTRKEWIDSLTNSGMRADKARFAVDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAE
IAGQLLAGKTLKKEQMRAVTEIVTSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKA
LEDDGLPARTVAAFLKAKDKKLDEKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAG
KPFEQLIKAGMQTSYMKDIQRQKNEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLS
SEQQDATLIISGTNASRKTLNDYIRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKN
GMKRGESYQVLDTGPGNKLTVESISGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGD
RFTVKTVEGEKLTLEDKKGRTVELDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVG
ISRARHEVEIFTDDKKSLASSVSRDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESM
AFNQKPDEHNMTTGTDYQPVSNAEDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYA
DYEHYAEASDYDFDSSIYDDYAMPQTSQAEQSHTGKEHTHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(734-778)

AAA domain (AAA_30)

(360-536)

TrwC relaxase (TrwC)

(4-170)


  Protein structure



No available structure.




T4CP

ID   3826 GenBank   WP_032201036
Name   t4cp2_EKN58_RS23280_pCTXM65_090011 insolico UniProt ID   _
Length   916 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 916 a.a.        Molecular weight: 105100.60 Da        Isoelectric Point: 7.6881

>WP_032201036.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacteriaceae]
MTILNKALTRPAALWGVPLVPLLFTLCILTLAAVWSRFYWLLLLLIPAFIELRRLAKEDIHFFDLKLLAF
KTRGNRAATRHFGTKALVANQYDAIDVTEFIKHMRLNESIPLSRYLPWSSHLHEQVVKNKKGDFVSTWEI
GGIIFECEDEHYLATLDMQLNNVIRSFEGLPVTFYTHLVSDEFTDTLECESGIPFSDEIARLFYASREGK
PFYQRRLFFTVCFNPHTGIERATMKQKSLGERKTLLDNALQVMQEHRQAIGTALSRYAARPLGTYEENGH
VFSSQLAFYNFLITGRWQKVRVTRTPFYDVLGGTDIFPSTDIGQVSHTDGQSYFRGLEIKDYSTETITGL
YDSLLYARCRYVFTQSYTCMGKDEARGCIRTIEKRLKSAEDDAISQQEDLKVARDMLQSGIISFGKYHQT
LIVSAPTSEQVIRDTNTLADTLKDLGFIMTLSRESLSAAYLAQLPGVYHLRPRLVPVSSLNYAEMVSFHN
FHPHKRDGNAWGEAIAVVPTPSGGCQYVNLHNSQADVDEFSEKPPGNTMLLGSTGTGKSTLLMMFQHLMQ
KYRRPESFAPGATKKRFTSVVFDKDRSAEMSIRLQGGSYFRFRSGEKTGLNPFALAPTKRNRNFLKKLMR
MMCTRDGKPLDPRDEERLSEAVDTIMLDYPPELRRNGITRMLEVLHEAPTKDARMNGLRIRLQQWAQGGE
FGWVFDNDVDTFDIDNIDNFGIDGTEFLNDKDVRSPLTFYLLYRVTSLLDGRRLVIFMDEFWKWLQDPEF
SKFALDMLKVIRKLNGIFFPATQSPEEMCKHEISAAIIEQCSTRIFMANQQAKEYDYVDMLRVPKAVYDI
VTTLDPYARQMVIMKSPLRRGDVRPYIAQITLDLSGLGRYTKLLSASADNLKIFDEIWQEGMKPEDWKDE
MLRRAV

  Protein domains


Predicted by InterproScan.

Type IV secretory pathway, VirB3-like protein (VirB3)

(4-81)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(272-473)

TraG P-loop domain (P-loop_TraG)

(537-821)

  Protein structure



No available structure.



ID   3827 GenBank   WP_000575554
Name   t4cp2_EKN58_RS23350_pCTXM65_090011 insolico UniProt ID   _
Length   635 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 635 a.a.        Molecular weight: 70289.70 Da        Isoelectric Point: 6.1172

>WP_000575554.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MIFDKLNHLLSPIANAVLAFIAFLQAHQLALALLSGVMLPFIFSAKHGGQEKPTFLQRILILLSMICFVF
GSIAPVAVWFLQRIYGREDIASPPLLTWTMALLFTVAGFILHVVLRRLISPEFDKVKCGMVKKTSMERDR
RTDVRRVKEILPVTTEYDPMDYIDLNKGIFIGLSREGEPQYIPLKEWQTQHADIIGTTGAGKGVVTGILL
YQSILADEGVFVLDPKNDEWAPHLYKKACADAGKPFVLIDLNKQEYQMNLIDGITADHLEELFVAGFSLA
EKGEAADFYRIDDRKAARTAAQFVKDNPGATVRDIYNGDYVQGIGETIKAFHGKTEELAMLNSINAPGGF
ALKEIFDKGGCCYIIGSMRNSKIITAQRMILVRLFQLAELRDRVAGNIRPIAIFKDEVKYHLSKPALEGL
GAARDKGVHILMAHQSIADLRDCPADLNGDAVVGAVVENCKFKLVYKLQDPDTAEWIARMSGTILVDDES
RKVTTTAVLTEKVDNERMLRQAESYYIDSNMLQNLPKFVSYIFSQNALAFPSLISPLKVKKAPLELFTLP
PVAVATVAEVKPVLDLDFDKTAPAVPAPDIIPVSATTTKKPSATPIPTPPVQPDEEWLMASQPAPNDEIH
SLLDL

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(402-496)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 74907..91368

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKN58_RS23240 (EKN58_023240) 69977..71695 - 1719 WP_000739198 hybrid sensor histidine kinase/response regulator -
EKN58_RS23245 (EKN58_023245) 71692..72180 - 489 WP_000238872 molybdopterin-dependent oxidoreductase -
EKN58_RS23250 (EKN58_023250) 72173..73156 - 984 WP_032201035 hypothetical protein -
EKN58_RS23255 (EKN58_023255) 73160..74074 - 915 WP_000891983 helix-turn-helix domain-containing protein -
EKN58_RS23260 (EKN58_023260) 74388..74696 + 309 WP_064195765 hypothetical protein -
EKN58_RS23265 (EKN58_023265) 74689..74904 + 216 WP_001112907 hypothetical protein -
EKN58_RS23270 (EKN58_023270) 74907..75569 + 663 WP_000958205 lytic transglycosylase domain-containing protein virB1
EKN58_RS23275 (EKN58_023275) 75580..75879 + 300 WP_000175462 TrbC/VirB2 family protein virB2
EKN58_RS23280 (EKN58_023280) 75889..78639 + 2751 WP_032201036 VirB3 family type IV secretion system protein virb4
EKN58_RS23285 (EKN58_023285) 78658..79374 + 717 WP_000770034 type IV secretion system protein -
EKN58_RS23290 (EKN58_023290) 79386..79670 + 285 WP_000769859 EexN family lipoprotein -
EKN58_RS23295 (EKN58_023295) 79688..80695 + 1008 WP_032201037 type IV secretion system protein virB6
EKN58_RS23890 80772..80912 + 141 WP_000873539 hypothetical protein -
EKN58_RS23300 (EKN58_023300) 80905..81591 + 687 WP_001293055 type IV secretion system protein virB8
EKN58_RS23305 (EKN58_023305) 81584..82477 + 894 WP_001045307 P-type conjugative transfer protein VirB9 virB9
EKN58_RS23310 (EKN58_023310) 82474..83670 + 1197 WP_000980841 VirB10/TraB/TrbI family type IV secretion system protein -
EKN58_RS23315 (EKN58_023315) 83674..84723 + 1050 WP_000342114 P-type DNA transfer ATPase VirB11 virB11
EKN58_RS23320 (EKN58_023320) 84710..85111 + 402 WP_000979494 cag pathogenicity island Cag12 family protein -
EKN58_RS23325 (EKN58_023325) 85202..85519 + 318 WP_000683352 TrbM/KikA/MpfK family conjugal transfer protein -
EKN58_RS23330 (EKN58_023330) 85546..85881 + 336 WP_000610141 hypothetical protein -
EKN58_RS23335 (EKN58_023335) 85857..86165 + 309 WP_000212005 hypothetical protein -
EKN58_RS23340 (EKN58_023340) 86162..88363 + 2202 WP_001241818 type IA DNA topoisomerase -
EKN58_RS23345 (EKN58_023345) 88360..88752 + 393 WP_001079960 H-NS family nucleoid-associated regulatory protein -
EKN58_RS23350 (EKN58_023350) 89461..91368 + 1908 WP_000575554 type IV secretion system DNA-binding domain-containing protein virb4
EKN58_RS23355 (EKN58_023355) 91383..92129 + 747 WP_000909125 MobC family replication-relaxation protein -
EKN58_RS23360 (EKN58_023360) 92122..92649 + 528 WP_001232861 phospholipase D family protein -
EKN58_RS23365 (EKN58_023365) 92681..93112 - 432 WP_001531161 hypothetical protein -
EKN58_RS23370 (EKN58_023370) 93592..94557 - 966 WP_001743098 zincin-like metallopeptidase domain-containing protein -
EKN58_RS23375 (EKN58_023375) 95012..96232 - 1221 Protein_104 ISL3-like element ISKox3 family transposase -

Host bacterium

ID   6066 GenBank   NZ_CP036311
Plasmid name   pCTXM65_090011 Incompatibility group   IncFIB
Plasmid size   102543 bp Coordinate of oriT [Strand]   42229..42323 [-]
Host baterium   Enterobacter hormaechei strain WCHEH090011

Cargo genes

Drug resistance gene   blaCTX-M-65, sul1, qacE, aadA16, dfrA27, ARR-3, aac(6')-Ib-cr, blaTEM-1B, tet(A)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -