Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105626
Name   oriT_AR_038|unnamed5 in_silico
Organism   Enterobacter hormaechei strain AR_038
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP030350 (40574..40660 [+], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_AR_038|unnamed5
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3817 GenBank   WP_022650008
Name   Relaxase_CSB67_RS26300_AR_038|unnamed5 insolico UniProt ID   A0A4Q2QTK9
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102314.37 Da        Isoelectric Point: 8.2756

>WP_022650008.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(51-317)


  Protein structure


Source ID Structure
AlphaFold DB A0A4Q2QTK9


T4CP

ID   3823 GenBank   WP_022650006
Name   trbC_CSB67_RS26290_AR_038|unnamed5 insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87660.94 Da        Isoelectric Point: 6.8660

>WP_022650006.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 7..34451

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSB67_RS26145 (CSB67_5178) 7..1566 + 1560 WP_022649975 ATPase, T2SS/T4P/T4SS family virB11
CSB67_RS26150 (CSB67_5179) 1559..2653 + 1095 WP_032623575 type II secretion system F family protein -
CSB67_RS26155 (CSB67_5180) 2667..3281 + 615 WP_022649977 type 4 pilus major pilin -
CSB67_RS26160 (CSB67_5181) 3366..3848 + 483 WP_045261878 lytic transglycosylase domain-containing protein virB1
CSB67_RS26165 3833..4507 + 675 WP_022649979 prepilin peptidase -
CSB67_RS26170 (CSB67_5182) 4504..5841 + 1338 WP_111978152 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
CSB67_RS26175 (CSB67_5183) 6419..7579 + 1161 WP_022649981 site-specific integrase -
CSB67_RS26805 7599..8075 + 477 WP_022649982 hypothetical protein -
CSB67_RS26180 (CSB67_5184) 8222..9058 + 837 WP_022649983 hypothetical protein traE
CSB67_RS26185 (CSB67_5185) 9164..10369 + 1206 WP_022649984 conjugal transfer protein TraF -
CSB67_RS26810 10478..10894 + 417 WP_123839394 hypothetical protein -
CSB67_RS26190 (CSB67_5187) 10965..11438 + 474 WP_022649985 DotD/TraH family lipoprotein -
CSB67_RS26195 (CSB67_5188) 11435..12241 + 807 WP_022649986 type IV secretory system conjugative DNA transfer family protein traI
CSB67_RS26200 (CSB67_5189) 12254..13414 + 1161 WP_022649987 plasmid transfer ATPase TraJ virB11
CSB67_RS26205 (CSB67_5190) 13411..13698 + 288 WP_022649988 hypothetical protein traK
CSB67_RS26210 (CSB67_5191) 13770..17312 + 3543 WP_032635082 LPD7 domain-containing protein -
CSB67_RS26215 (CSB67_5192) 17330..17680 + 351 WP_022649990 hypothetical protein traL
CSB67_RS26220 (CSB67_5193) 17770..18387 + 618 WP_179297403 DotI/IcmL family type IV secretion protein traM
CSB67_RS26225 (CSB67_5194) 18398..19378 + 981 WP_022649992 DotH/IcmK family type IV secretion protein traN
CSB67_RS26230 (CSB67_5195) 19378..20685 + 1308 WP_022649993 conjugal transfer protein TraO traO
CSB67_RS26235 (CSB67_5196) 20666..21439 + 774 WP_123839398 conjugal transfer protein TraP traP
CSB67_RS26240 21439..21963 + 525 WP_022649995 conjugal transfer protein TraQ traQ
CSB67_RS26245 (CSB67_5198) 21998..22402 + 405 WP_022649996 DUF6750 family protein traR
CSB67_RS26250 (CSB67_5199) 22586..23311 + 726 WP_063864836 hypothetical protein traT
CSB67_RS26255 (CSB67_5200) 23398..26451 + 3054 WP_022649998 hypothetical protein traU
CSB67_RS26990 (CSB67_5201) 26451..27020 + 570 WP_022649999 hypothetical protein traV
CSB67_RS26260 (CSB67_5202) 27033..28235 + 1203 WP_022650000 conjugal transfer protein TraW traW
CSB67_RS26265 (CSB67_5203) 28232..28774 + 543 WP_022650001 conjugal transfer protein TraX -
CSB67_RS26270 (CSB67_5204) 28832..30991 + 2160 WP_022650002 DotA/TraY family protein traY
CSB67_RS26275 31053..31685 + 633 WP_022650003 plasmid IncI1-type surface exclusion protein ExcA -
CSB67_RS26280 (CSB67_5206) 32006..33214 + 1209 WP_022650004 hypothetical protein trbA
CSB67_RS26285 (CSB67_5207) 33267..34451 + 1185 WP_022650005 hypothetical protein trbB
CSB67_RS26290 (CSB67_5208) 34455..36755 + 2301 WP_022650006 F-type conjugative transfer protein TrbC -
CSB67_RS26295 (CSB67_5209) 36829..37098 + 270 WP_022650007 hypothetical protein -
CSB67_RS26815 37232..37435 + 204 WP_123839396 hypothetical protein -

Host bacterium

ID   6063 GenBank   NZ_CP030350
Plasmid name   AR_038|unnamed5 Incompatibility group   -
Plasmid size   106698 bp Coordinate of oriT [Strand]   40574..40660 [+]
Host baterium   Enterobacter hormaechei strain AR_038

Cargo genes

Drug resistance gene   qnrS1, blaLAP-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA7, AcrIIC1