Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105613 |
| Name | oriT_pCAV1668-85  |
| Organism | Enterobacter hormaechei strain CAV1668 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP011583 (16765..16858 [-], 94 nt) |
| oriT length | 94 nt |
| IRs (inverted repeats) | 73..78, 84..89 (AAAAAA..TTTTTT) 27..34, 37..44 (AGCGTGAT..ATCACGCT) 13..19, 31..37 (TAAATCA..TGATTTA) |
| Location of nic site | 55..56 |
| Conserved sequence flanking the nic site |
GGTGTATAGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 94 nt
>oriT_pCAV1668-85
TTTTTTTTCTTTTAAATCAGTGGTATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
TTTTTTTTCTTTTAAATCAGTGGTATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 3813 | GenBank | WP_029074264 |
| Name | t4cp2_ABY64_RS00590_pCAV1668-85 |
UniProt ID | _ |
| Length | 662 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 662 a.a. Molecular weight: 73142.66 Da Isoelectric Point: 6.5145
>WP_029074264.1 MULTISPECIES: conjugal transfer protein TraG [Pseudomonadota]
MRGGRILWGQIAVVFTIVLVMTWAATQWVAFRLGFQPQLGNPWFELVGLPVYYPPAFFWWWFSFDAYAPA
IFVEGGIIAVSGGFLAIAAAILMSIIRAREARNVATYGSARWAEDREIRTAGLLGPDGVLLGRYNRDYLR
HDGPEHVLCFAPTRSGKGVGLVVPTLLTWSASAIVHDIKGENWTLTAGFRAKHGRVLLFDPTNARSSAYN
PLLEVRQGEWEVRDVQNIADILVDPEGSLDKRNHWEKTSHSLLVGAILHVLYAEKDKTLAGVANFLSDPR
RPVEATLRAMMDTPHLGEAGVHPVIASSARELLNKSENERSGVLSTAMSFLGLYRDPVVAQVTARCDWRI
ADLVGSRRPVTLYLVVPPSDINRTKPLIRLILNQIGRRLTEELTTSGKRHRLLLMLDEFPALGRLDFFES
ALAFMAGYGIKGFLIAQSLNQIERAYGPNNAILDNCHVRVSFATNDERTAKRVSDALGTATELRDSTNYA
GHRLAPWLGHLMVSRQETARPLLTPGEIMQLPPTDEIVMVAGTPPIRATKARYFEDARFQERILTPPDLV
AAPLAPSPSADDWSGRVVAAGSRSATVAADATEGDPANAGIRREPELPEHEEIVTPPPSPEQEFDILDDE
PDVDAAKARTIRQRMRMVARQVAMNPDDGIEL
MRGGRILWGQIAVVFTIVLVMTWAATQWVAFRLGFQPQLGNPWFELVGLPVYYPPAFFWWWFSFDAYAPA
IFVEGGIIAVSGGFLAIAAAILMSIIRAREARNVATYGSARWAEDREIRTAGLLGPDGVLLGRYNRDYLR
HDGPEHVLCFAPTRSGKGVGLVVPTLLTWSASAIVHDIKGENWTLTAGFRAKHGRVLLFDPTNARSSAYN
PLLEVRQGEWEVRDVQNIADILVDPEGSLDKRNHWEKTSHSLLVGAILHVLYAEKDKTLAGVANFLSDPR
RPVEATLRAMMDTPHLGEAGVHPVIASSARELLNKSENERSGVLSTAMSFLGLYRDPVVAQVTARCDWRI
ADLVGSRRPVTLYLVVPPSDINRTKPLIRLILNQIGRRLTEELTTSGKRHRLLLMLDEFPALGRLDFFES
ALAFMAGYGIKGFLIAQSLNQIERAYGPNNAILDNCHVRVSFATNDERTAKRVSDALGTATELRDSTNYA
GHRLAPWLGHLMVSRQETARPLLTPGEIMQLPPTDEIVMVAGTPPIRATKARYFEDARFQERILTPPDLV
AAPLAPSPSADDWSGRVVAAGSRSATVAADATEGDPANAGIRREPELPEHEEIVTPPPSPEQEFDILDDE
PDVDAAKARTIRQRMRMVARQVAMNPDDGIEL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 3814 | GenBank | WP_029074259 |
| Name | trbE_ABY64_RS00620_pCAV1668-85 |
UniProt ID | _ |
| Length | 830 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 830 a.a. Molecular weight: 90719.49 Da Isoelectric Point: 5.3930
>WP_029074259.1 MULTISPECIES: conjugal transfer protein TrbE [Pseudomonadota]
MMNLSEYRRTAARLADYLPWAALLGSGVVLNKDGSFQRTAKFRGPDLDSAVAAELVAVAGRINNAVRRLG
SGWSIFVEAQRSEAATYPDSTFPDAASALVDAERKAGFEEAGTHFVSGYFLTFLWLPPAEEAARAETWLY
EGREKTGVDPWELLRGFIDRTDRVLALLDGFMPECRWMDDGATLTYLHSTISTNRHRVRVPEVPMHLDAL
LADQPLTGGLEPRLGTKHLRVLTIVGFPTATTPGLLDEMNRLPFPYRWSTRAILLDKTDATRLLTRIRRQ
WFAKRKSIAAILKEVMTNEASALVDTDAANKALDADMALQELGADVAGMAYVTATVTVWDADPRLADEKL
RLVEKIVQGRDFTAMPESVNAVDAWLGSLPGHAYANVRQPPISTLNLAHMIPLSAVWAGPERDEHFGAPP
LLFGKTEGSTPFRLSLHVGDVGHTLVVGPTGAGKSVLLALMALQFRRYAGSQVFAFDFGGSIRAAALAMG
GDWHDLGGGLTEGAEASVSLQPLARIHDTYERAWAADWIAAILMREGIAITPEVKEYLWTALTSLASAPV
EERTITGLAVLLQSNDLKQALRPFCVGGAYGRLLDAEAEHLGSADVQAFEIEGLVGTGAAPAVLSYLFHR
IGDRLDGRPTLLIIDEGWLALDDEGFAGQLREWLKTLRKKNASVIFATQSLSDIDGSNIAPAIIESCPTR
LLLPNERAIEPQITEIYRRFGLNDRQIEILARATPKRDYYCQSRRGNRLFELGLSEIGLALCAASAKSDQ
TLIAQIVAEHGRDGFLAAWLRARGVEWAAELIPNLTNLADRPESAAPARLDTHPTQEILP
MMNLSEYRRTAARLADYLPWAALLGSGVVLNKDGSFQRTAKFRGPDLDSAVAAELVAVAGRINNAVRRLG
SGWSIFVEAQRSEAATYPDSTFPDAASALVDAERKAGFEEAGTHFVSGYFLTFLWLPPAEEAARAETWLY
EGREKTGVDPWELLRGFIDRTDRVLALLDGFMPECRWMDDGATLTYLHSTISTNRHRVRVPEVPMHLDAL
LADQPLTGGLEPRLGTKHLRVLTIVGFPTATTPGLLDEMNRLPFPYRWSTRAILLDKTDATRLLTRIRRQ
WFAKRKSIAAILKEVMTNEASALVDTDAANKALDADMALQELGADVAGMAYVTATVTVWDADPRLADEKL
RLVEKIVQGRDFTAMPESVNAVDAWLGSLPGHAYANVRQPPISTLNLAHMIPLSAVWAGPERDEHFGAPP
LLFGKTEGSTPFRLSLHVGDVGHTLVVGPTGAGKSVLLALMALQFRRYAGSQVFAFDFGGSIRAAALAMG
GDWHDLGGGLTEGAEASVSLQPLARIHDTYERAWAADWIAAILMREGIAITPEVKEYLWTALTSLASAPV
EERTITGLAVLLQSNDLKQALRPFCVGGAYGRLLDAEAEHLGSADVQAFEIEGLVGTGAAPAVLSYLFHR
IGDRLDGRPTLLIIDEGWLALDDEGFAGQLREWLKTLRKKNASVIFATQSLSDIDGSNIAPAIIESCPTR
LLLPNERAIEPQITEIYRRFGLNDRQIEILARATPKRDYYCQSRRGNRLFELGLSEIGLALCAASAKSDQ
TLIAQIVAEHGRDGFLAAWLRARGVEWAAELIPNLTNLADRPESAAPARLDTHPTQEILP
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 66590..76326
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ABY64_RS00585 (ABY64_00580) | 62164..62670 | - | 507 | WP_031623797 | helix-turn-helix domain-containing protein | - |
| ABY64_RS00590 (ABY64_00585) | 62940..64928 | + | 1989 | WP_029074264 | conjugal transfer protein TraG | - |
| ABY64_RS00595 (ABY64_00590) | 64939..65412 | + | 474 | WP_029074263 | hypothetical protein | - |
| ABY64_RS00600 (ABY64_00595) | 65489..66253 | - | 765 | WP_158581548 | alpha/beta fold hydrolase | - |
| ABY64_RS25160 | 66321..66443 | + | 123 | Protein_76 | CopG family transcriptional regulator | - |
| ABY64_RS00605 (ABY64_00600) | 66590..67585 | + | 996 | WP_029074262 | P-type conjugative transfer ATPase TrbB | virB11 |
| ABY64_RS00610 (ABY64_00605) | 67582..67914 | + | 333 | WP_029074261 | TrbC/VirB2 family protein | virB2 |
| ABY64_RS00615 (ABY64_00610) | 67914..68195 | + | 282 | WP_029074260 | VirB3 family type IV secretion system protein | virB3 |
| ABY64_RS00620 (ABY64_00615) | 68206..70698 | + | 2493 | WP_029074259 | conjugal transfer protein TrbE | virb4 |
| ABY64_RS00625 (ABY64_00620) | 70695..71471 | + | 777 | WP_029074258 | P-type conjugative transfer protein TrbJ | virB5 |
| ABY64_RS00630 (ABY64_00625) | 71486..71752 | + | 267 | WP_031623918 | putative entry exclusion protein TrbK-alt | - |
| ABY64_RS00635 (ABY64_00630) | 71755..73110 | + | 1356 | WP_029074256 | P-type conjugative transfer protein TrbL | virB6 |
| ABY64_RS00640 (ABY64_00635) | 73107..73796 | + | 690 | WP_031623919 | conjugal transfer protein TrbF | virB8 |
| ABY64_RS00645 (ABY64_00640) | 73802..74179 | - | 378 | WP_029074878 | nuclear transport factor 2 family protein | - |
| ABY64_RS00650 (ABY64_00645) | 74276..75193 | + | 918 | WP_200523655 | P-type conjugative transfer protein TrbG | virB9 |
| ABY64_RS00655 (ABY64_00650) | 75190..76326 | + | 1137 | WP_031623920 | TrbI/VirB10 family protein | virB10 |
| ABY64_RS00660 (ABY64_00655) | 76329..76550 | + | 222 | WP_029074252 | DUF2274 domain-containing protein | - |
| ABY64_RS00665 (ABY64_00660) | 76686..77003 | + | 318 | WP_029074251 | hypothetical protein | - |
| ABY64_RS00670 (ABY64_00665) | 77000..77353 | + | 354 | WP_029074250 | DUF6516 family protein | - |
| ABY64_RS00675 (ABY64_00670) | 77350..77748 | + | 399 | WP_029074249 | helix-turn-helix domain-containing protein | - |
| ABY64_RS00680 (ABY64_00675) | 77745..79391 | + | 1647 | WP_031623921 | recombinase family protein | - |
| ABY64_RS00685 (ABY64_00680) | 79372..79695 | + | 324 | Protein_93 | IS21 family transposase | - |
| ABY64_RS00690 (ABY64_00685) | 79682..80467 | + | 786 | WP_000983249 | IS21-like element IS1326 family helper ATPase IstB | - |
Host bacterium
| ID | 6051 | GenBank | NZ_CP011583 |
| Plasmid name | pCAV1668-85 | Incompatibility group | IncR |
| Plasmid size | 85187 bp | Coordinate of oriT [Strand] | 16765..16858 [-] |
| Host baterium | Enterobacter hormaechei strain CAV1668 |
Cargo genes
| Drug resistance gene | blaTEM-1B, floR, tet(G), sul1, qacE, ant(3'')-Ia |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merB, merR2 |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |