Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105611 |
| Name | oriT_pKPC_CAV1411  |
| Organism | Enterobacter hormaechei strain CAV1411 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP011580 (11998..12091 [+], 94 nt) |
| oriT length | 94 nt |
| IRs (inverted repeats) | 73..78, 84..89 (AAAAAA..TTTTTT) 27..34, 37..44 (AGCGTGAT..ATCACGCT) 13..19, 31..37 (TAAATCA..TGATTTA) |
| Location of nic site | 55..56 |
| Conserved sequence flanking the nic site |
GGTGTATAGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 94 nt
>oriT_pKPC_CAV1411
TTTTTTTTCTTTTAAATCAGTGGTATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
TTTTTTTTCTTTTAAATCAGTGGTATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 3809 | GenBank | WP_029074259 |
| Name | trbE_ABY65_RS00495_pKPC_CAV1411 |
UniProt ID | _ |
| Length | 830 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 830 a.a. Molecular weight: 90719.49 Da Isoelectric Point: 5.3930
>WP_029074259.1 MULTISPECIES: conjugal transfer protein TrbE [Pseudomonadota]
MMNLSEYRRTAARLADYLPWAALLGSGVVLNKDGSFQRTAKFRGPDLDSAVAAELVAVAGRINNAVRRLG
SGWSIFVEAQRSEAATYPDSTFPDAASALVDAERKAGFEEAGTHFVSGYFLTFLWLPPAEEAARAETWLY
EGREKTGVDPWELLRGFIDRTDRVLALLDGFMPECRWMDDGATLTYLHSTISTNRHRVRVPEVPMHLDAL
LADQPLTGGLEPRLGTKHLRVLTIVGFPTATTPGLLDEMNRLPFPYRWSTRAILLDKTDATRLLTRIRRQ
WFAKRKSIAAILKEVMTNEASALVDTDAANKALDADMALQELGADVAGMAYVTATVTVWDADPRLADEKL
RLVEKIVQGRDFTAMPESVNAVDAWLGSLPGHAYANVRQPPISTLNLAHMIPLSAVWAGPERDEHFGAPP
LLFGKTEGSTPFRLSLHVGDVGHTLVVGPTGAGKSVLLALMALQFRRYAGSQVFAFDFGGSIRAAALAMG
GDWHDLGGGLTEGAEASVSLQPLARIHDTYERAWAADWIAAILMREGIAITPEVKEYLWTALTSLASAPV
EERTITGLAVLLQSNDLKQALRPFCVGGAYGRLLDAEAEHLGSADVQAFEIEGLVGTGAAPAVLSYLFHR
IGDRLDGRPTLLIIDEGWLALDDEGFAGQLREWLKTLRKKNASVIFATQSLSDIDGSNIAPAIIESCPTR
LLLPNERAIEPQITEIYRRFGLNDRQIEILARATPKRDYYCQSRRGNRLFELGLSEIGLALCAASAKSDQ
TLIAQIVAEHGRDGFLAAWLRARGVEWAAELIPNLTNLADRPESAAPARLDTHPTQEILP
MMNLSEYRRTAARLADYLPWAALLGSGVVLNKDGSFQRTAKFRGPDLDSAVAAELVAVAGRINNAVRRLG
SGWSIFVEAQRSEAATYPDSTFPDAASALVDAERKAGFEEAGTHFVSGYFLTFLWLPPAEEAARAETWLY
EGREKTGVDPWELLRGFIDRTDRVLALLDGFMPECRWMDDGATLTYLHSTISTNRHRVRVPEVPMHLDAL
LADQPLTGGLEPRLGTKHLRVLTIVGFPTATTPGLLDEMNRLPFPYRWSTRAILLDKTDATRLLTRIRRQ
WFAKRKSIAAILKEVMTNEASALVDTDAANKALDADMALQELGADVAGMAYVTATVTVWDADPRLADEKL
RLVEKIVQGRDFTAMPESVNAVDAWLGSLPGHAYANVRQPPISTLNLAHMIPLSAVWAGPERDEHFGAPP
LLFGKTEGSTPFRLSLHVGDVGHTLVVGPTGAGKSVLLALMALQFRRYAGSQVFAFDFGGSIRAAALAMG
GDWHDLGGGLTEGAEASVSLQPLARIHDTYERAWAADWIAAILMREGIAITPEVKEYLWTALTSLASAPV
EERTITGLAVLLQSNDLKQALRPFCVGGAYGRLLDAEAEHLGSADVQAFEIEGLVGTGAAPAVLSYLFHR
IGDRLDGRPTLLIIDEGWLALDDEGFAGQLREWLKTLRKKNASVIFATQSLSDIDGSNIAPAIIESCPTR
LLLPNERAIEPQITEIYRRFGLNDRQIEILARATPKRDYYCQSRRGNRLFELGLSEIGLALCAASAKSDQ
TLIAQIVAEHGRDGFLAAWLRARGVEWAAELIPNLTNLADRPESAAPARLDTHPTQEILP
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 3810 | GenBank | WP_029074264 |
| Name | t4cp2_ABY65_RS00525_pKPC_CAV1411 |
UniProt ID | _ |
| Length | 662 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 662 a.a. Molecular weight: 73142.66 Da Isoelectric Point: 6.5145
>WP_029074264.1 MULTISPECIES: conjugal transfer protein TraG [Pseudomonadota]
MRGGRILWGQIAVVFTIVLVMTWAATQWVAFRLGFQPQLGNPWFELVGLPVYYPPAFFWWWFSFDAYAPA
IFVEGGIIAVSGGFLAIAAAILMSIIRAREARNVATYGSARWAEDREIRTAGLLGPDGVLLGRYNRDYLR
HDGPEHVLCFAPTRSGKGVGLVVPTLLTWSASAIVHDIKGENWTLTAGFRAKHGRVLLFDPTNARSSAYN
PLLEVRQGEWEVRDVQNIADILVDPEGSLDKRNHWEKTSHSLLVGAILHVLYAEKDKTLAGVANFLSDPR
RPVEATLRAMMDTPHLGEAGVHPVIASSARELLNKSENERSGVLSTAMSFLGLYRDPVVAQVTARCDWRI
ADLVGSRRPVTLYLVVPPSDINRTKPLIRLILNQIGRRLTEELTTSGKRHRLLLMLDEFPALGRLDFFES
ALAFMAGYGIKGFLIAQSLNQIERAYGPNNAILDNCHVRVSFATNDERTAKRVSDALGTATELRDSTNYA
GHRLAPWLGHLMVSRQETARPLLTPGEIMQLPPTDEIVMVAGTPPIRATKARYFEDARFQERILTPPDLV
AAPLAPSPSADDWSGRVVAAGSRSATVAADATEGDPANAGIRREPELPEHEEIVTPPPSPEQEFDILDDE
PDVDAAKARTIRQRMRMVARQVAMNPDDGIEL
MRGGRILWGQIAVVFTIVLVMTWAATQWVAFRLGFQPQLGNPWFELVGLPVYYPPAFFWWWFSFDAYAPA
IFVEGGIIAVSGGFLAIAAAILMSIIRAREARNVATYGSARWAEDREIRTAGLLGPDGVLLGRYNRDYLR
HDGPEHVLCFAPTRSGKGVGLVVPTLLTWSASAIVHDIKGENWTLTAGFRAKHGRVLLFDPTNARSSAYN
PLLEVRQGEWEVRDVQNIADILVDPEGSLDKRNHWEKTSHSLLVGAILHVLYAEKDKTLAGVANFLSDPR
RPVEATLRAMMDTPHLGEAGVHPVIASSARELLNKSENERSGVLSTAMSFLGLYRDPVVAQVTARCDWRI
ADLVGSRRPVTLYLVVPPSDINRTKPLIRLILNQIGRRLTEELTTSGKRHRLLLMLDEFPALGRLDFFES
ALAFMAGYGIKGFLIAQSLNQIERAYGPNNAILDNCHVRVSFATNDERTAKRVSDALGTATELRDSTNYA
GHRLAPWLGHLMVSRQETARPLLTPGEIMQLPPTDEIVMVAGTPPIRATKARYFEDARFQERILTPPDLV
AAPLAPSPSADDWSGRVVAAGSRSATVAADATEGDPANAGIRREPELPEHEEIVTPPPSPEQEFDILDDE
PDVDAAKARTIRQRMRMVARQVAMNPDDGIEL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 47540..57276
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ABY65_RS00425 (ABY65_00420) | 43399..44184 | - | 786 | WP_000983249 | IS21-like element IS1326 family helper ATPase IstB | - |
| ABY65_RS00430 (ABY65_00425) | 44171..44494 | - | 324 | Protein_45 | IS21 family transposase | - |
| ABY65_RS00435 (ABY65_00430) | 44475..46121 | - | 1647 | WP_031623921 | recombinase family protein | - |
| ABY65_RS00440 (ABY65_00435) | 46118..46516 | - | 399 | WP_029074249 | helix-turn-helix domain-containing protein | - |
| ABY65_RS00445 (ABY65_00440) | 46513..46866 | - | 354 | WP_029074250 | DUF6516 family protein | - |
| ABY65_RS00450 (ABY65_00445) | 46863..47180 | - | 318 | WP_029074251 | hypothetical protein | - |
| ABY65_RS00455 (ABY65_00450) | 47316..47537 | - | 222 | WP_029074252 | DUF2274 domain-containing protein | - |
| ABY65_RS00460 (ABY65_00455) | 47540..48676 | - | 1137 | WP_031623920 | TrbI/VirB10 family protein | virB10 |
| ABY65_RS00465 (ABY65_00460) | 48673..49590 | - | 918 | WP_200523655 | P-type conjugative transfer protein TrbG | virB9 |
| ABY65_RS00470 (ABY65_00465) | 49687..50064 | + | 378 | WP_029074878 | nuclear transport factor 2 family protein | - |
| ABY65_RS00475 (ABY65_00470) | 50070..50759 | - | 690 | WP_031623919 | conjugal transfer protein TrbF | virB8 |
| ABY65_RS00480 (ABY65_00475) | 50756..52111 | - | 1356 | WP_029074256 | P-type conjugative transfer protein TrbL | virB6 |
| ABY65_RS00485 (ABY65_00480) | 52114..52380 | - | 267 | WP_031623918 | putative entry exclusion protein TrbK-alt | - |
| ABY65_RS00490 (ABY65_00485) | 52395..53171 | - | 777 | WP_029074258 | P-type conjugative transfer protein TrbJ | virB5 |
| ABY65_RS00495 (ABY65_00490) | 53168..55660 | - | 2493 | WP_029074259 | conjugal transfer protein TrbE | virb4 |
| ABY65_RS00500 (ABY65_00495) | 55671..55952 | - | 282 | WP_029074260 | VirB3 family type IV secretion system protein | virB3 |
| ABY65_RS00505 (ABY65_00500) | 55952..56284 | - | 333 | WP_029074261 | TrbC/VirB2 family protein | virB2 |
| ABY65_RS00510 (ABY65_00505) | 56281..57276 | - | 996 | WP_029074262 | P-type conjugative transfer ATPase TrbB | virB11 |
| ABY65_RS25160 | 57423..57545 | - | 123 | Protein_62 | CopG family transcriptional regulator | - |
| ABY65_RS00515 (ABY65_00510) | 57613..58377 | + | 765 | WP_158581548 | alpha/beta fold hydrolase | - |
| ABY65_RS00520 (ABY65_00515) | 58454..58927 | - | 474 | WP_029074263 | hypothetical protein | - |
| ABY65_RS00525 (ABY65_00520) | 58938..60926 | - | 1989 | WP_029074264 | conjugal transfer protein TraG | - |
| ABY65_RS00530 (ABY65_00525) | 61196..61702 | + | 507 | WP_031623797 | helix-turn-helix domain-containing protein | - |
Host bacterium
| ID | 6049 | GenBank | NZ_CP011580 |
| Plasmid name | pKPC_CAV1411 | Incompatibility group | IncR |
| Plasmid size | 90452 bp | Coordinate of oriT [Strand] | 11998..12091 [+] |
| Host baterium | Enterobacter hormaechei strain CAV1411 |
Cargo genes
| Drug resistance gene | blaKPC-2, blaTEM-1B, ant(3'')-Ia, qacE, sul1, tet(G), floR |
| Virulence gene | - |
| Metal resistance gene | merR2, merB, merD, merE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |