Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105595
Name   oriT_bat16|unnamed2 in_silico
Organism   Klebsiella pneumoniae strain bat16
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP092761 (94507..94556 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  GGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_bat16|unnamed2
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3805 GenBank   WP_240289378
Name   traI_MKZ45_RS27860_bat16|unnamed2 insolico UniProt ID   _
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190786.04 Da        Isoelectric Point: 6.4844

>WP_240289378.1 conjugative transfer relaxase/helicase TraI [Klebsiella pneumoniae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGMFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRHNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGASGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQKSVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNAPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKKAEQTAREMAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)

DNA helicase TraI (TraI_C)

(1433-1555)

TrwC relaxase (TrwC)

(10-284)

AAA domain (AAA_30)

(971-1157)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)


  Protein structure



No available structure.




T4CP

ID   3800 GenBank   WP_032495268
Name   traD_MKZ45_RS27865_bat16|unnamed2 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 85986.98 Da        Isoelectric Point: 5.0632

>WP_032495268.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEAREAEGSLARALFTPDTPEPGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 66117..95114

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ45_RS27865 (MKZ45_27855) 66117..68429 - 2313 WP_032495268 type IV conjugative transfer system coupling protein TraD virb4
MKZ45_RS27870 (MKZ45_27860) 68558..69247 - 690 WP_004198206 hypothetical protein -
MKZ45_RS27875 (MKZ45_27865) 69439..70170 - 732 WP_004152622 conjugal transfer complement resistance protein TraT -
MKZ45_RS27880 (MKZ45_27870) 70354..70902 - 549 WP_004152623 conjugal transfer entry exclusion protein TraS -
MKZ45_RS27885 (MKZ45_27875) 70915..73764 - 2850 WP_004152624 conjugal transfer mating-pair stabilization protein TraG traG
MKZ45_RS27890 (MKZ45_27880) 73764..75143 - 1380 WP_004165169 conjugal transfer pilus assembly protein TraH traH
MKZ45_RS27895 (MKZ45_27885) 75121..75564 - 444 WP_004152679 F-type conjugal transfer protein TrbF -
MKZ45_RS27900 (MKZ45_27890) 75610..76167 - 558 WP_004152678 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
MKZ45_RS27905 (MKZ45_27895) 76139..76378 - 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
MKZ45_RS27910 (MKZ45_27900) 76389..77141 - 753 WP_004152677 type-F conjugative transfer system pilin assembly protein TraF traF
MKZ45_RS27915 (MKZ45_27905) 77162..77488 - 327 WP_004152676 hypothetical protein -
MKZ45_RS27920 (MKZ45_27910) 77501..77749 - 249 WP_004152675 hypothetical protein -
MKZ45_RS27925 (MKZ45_27915) 77727..77981 - 255 WP_004152674 conjugal transfer protein TrbE -
MKZ45_RS27930 (MKZ45_27920) 78013..79968 - 1956 WP_004152673 type-F conjugative transfer system mating-pair stabilization protein TraN traN
MKZ45_RS27935 (MKZ45_27925) 80027..80665 - 639 WP_004152672 type-F conjugative transfer system pilin assembly protein TrbC trbC
MKZ45_RS27940 (MKZ45_27930) 80678..81667 - 990 WP_011977785 conjugal transfer pilus assembly protein TraU traU
MKZ45_RS27945 (MKZ45_27935) 81664..82053 - 390 WP_004152508 hypothetical protein -
MKZ45_RS27950 (MKZ45_27940) 82095..82721 - 627 WP_004152507 type-F conjugative transfer system protein TraW traW
MKZ45_RS27955 (MKZ45_27945) 82721..83110 - 390 WP_004152506 type-F conjugative transfer system protein TrbI -
MKZ45_RS27960 (MKZ45_27950) 83110..85749 - 2640 WP_004152505 type IV secretion system protein TraC virb4
MKZ45_RS27965 (MKZ45_27955) 85821..86219 - 399 WP_011977783 hypothetical protein -
MKZ45_RS27970 (MKZ45_27960) 86227..86616 - 390 WP_004153076 hypothetical protein -
MKZ45_RS27975 (MKZ45_27965) 86659..87063 - 405 WP_004152503 hypothetical protein -
MKZ45_RS27980 (MKZ45_27970) 87130..87441 - 312 WP_004152502 hypothetical protein -
MKZ45_RS27985 (MKZ45_27975) 87442..87660 - 219 WP_004152501 hypothetical protein -
MKZ45_RS27990 (MKZ45_27980) 87766..88176 - 411 WP_004152499 hypothetical protein -
MKZ45_RS27995 (MKZ45_27985) 88308..88892 - 585 WP_004161368 type IV conjugative transfer system lipoprotein TraV traV
MKZ45_RS28000 (MKZ45_27990) 89006..90430 - 1425 WP_004155033 F-type conjugal transfer pilus assembly protein TraB traB
MKZ45_RS28005 (MKZ45_27995) 90430..91170 - 741 WP_004152497 type-F conjugative transfer system secretin TraK traK
MKZ45_RS28010 (MKZ45_28000) 91157..91723 - 567 WP_004144423 type IV conjugative transfer system protein TraE traE
MKZ45_RS28015 (MKZ45_28005) 91743..92048 - 306 WP_004144424 type IV conjugative transfer system protein TraL traL
MKZ45_RS28020 (MKZ45_28010) 92062..92430 - 369 WP_004152496 type IV conjugative transfer system pilin TraA -
MKZ45_RS28025 (MKZ45_28015) 92484..92870 - 387 WP_004152495 TraY domain-containing protein -
MKZ45_RS28030 (MKZ45_28020) 92949..93635 - 687 WP_004152494 transcriptional regulator TraJ family protein -
MKZ45_RS28035 (MKZ45_28025) 93809..94207 - 399 WP_004152493 conjugal transfer relaxosome DNA-binding protein TraM -
MKZ45_RS28040 (MKZ45_28030) 94629..95114 + 486 WP_001568108 transglycosylase SLT domain-containing protein virB1
MKZ45_RS28045 (MKZ45_28035) 95147..95476 - 330 WP_011977736 DUF5983 family protein -
MKZ45_RS28050 (MKZ45_28040) 95509..96330 - 822 WP_004152492 DUF932 domain-containing protein -
MKZ45_RS28055 (MKZ45_28045) 97150..97983 - 834 WP_004152751 N-6 DNA methylase -
MKZ45_RS28060 (MKZ45_28050) 98034..98180 - 147 WP_004152750 hypothetical protein -
MKZ45_RS28065 (MKZ45_28055) 98275..98622 - 348 WP_004152749 hypothetical protein -
MKZ45_RS28070 (MKZ45_28060) 98679..99032 - 354 WP_004152748 hypothetical protein -
MKZ45_RS28075 (MKZ45_28065) 99662..100012 - 351 WP_004153414 hypothetical protein -

Host bacterium

ID   6033 GenBank   NZ_CP092761
Plasmid name   bat16|unnamed2 Incompatibility group   IncFIB
Plasmid size   118949 bp Coordinate of oriT [Strand]   94507..94556 [+]
Host baterium   Klebsiella pneumoniae strain bat16

Cargo genes

Drug resistance gene   blaTEM-1A, blaOXA-9, blaKPC-3
Virulence gene   -
Metal resistance gene   merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9